Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M104663200 on July 2, 2001

J. Biol. Chem., Vol. 276, Issue 35, 32729-32737, August 31, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/35/32729    most recent
M104663200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alvarez, I.
Right arrow Articles by de Castro, J. A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alvarez, I.
Right arrow Articles by de Castro, J. A. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Identification of Novel HLA-B27 Ligands Derived from Polymorphic Regions of Its Own or Other Class I Molecules Based on Direct Generation by 20 S Proteasome*

Iñaki AlvarezDagger , Laura SesmaDagger , Miguel MarcillaDagger , Manuel RamosDagger , Mercè MartíDagger , Emilio Camafeita§, and José A. López de CastroDagger

From the Dagger  Centro de Biología Molecular Severo Ochoa (C.S.I.C.-U.A.M.), Universidad Autónoma de Madrid, Facultad de Ciencias, and the § Centro Nacional de Biotecnología, 28049 Madrid, Spain

HLA-B27 is strongly associated with ankylosing spondylitis. Natural HLA-B27 ligands derived from polymorphic regions of its own or other class I HLA molecules might be involved in autoimmunity or provide diversity among HLA-B27-bound peptide repertoires from individuals. In particular, an 11-mer spanning HLA-B27 residues 169-179 is a natural HLA-B27 ligand with homology to proteins from Gram-negative bacteria. Proteasomal digestion of synthetic substrates demonstrated direct generation of the B27-(169-179) ligand. Cleavage after residue 181 generated a B27-(169-181) 13-mer that was subsequently found as a natural ligand of B*2705 and B*2704. Its binding to HLA-B27 subtypes in vivo correlated better than B27-(169-179) with association to spondyloarthropathy. Proteasomal cleavage generated also a peptide spanning B*2705 residues 150-158. This region is polymorphic among HLA-B27 subtypes and class I HLA antigens. The peptide was a natural B*2704 ligand. Since this subtype differs from B*2705 at residue 152, it was concluded that the ligand arose from HLA-B*3503, synthesized in the cells used as a source for B*2704-bound peptides. Thus, polymorphic HLA-B27 ligands derived from HLA-B27 or other class I molecules are directly produced by the 20 S proteasome in vitro, and this can be used for identification of such ligands in the constitutive HLA-B27-bound peptide pool.

* This work was supported by Grants SAF99/0055 from the Plan Nacional de I+D, PM99-0098 from the Ministry of Science and Technology, and 08.3/0022/1998 from the Comunidad Autónoma de Madrid and an institutional grant to the Centro de Biología Molecular Severo Ochoa from the Fundación Ramón Areces.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Facultad de Ciencias, Cantoblanco, 28049 Madrid, Spain. Tel.: 34-91-397-80-50; Fax: 34-91-397-80-87; E-Mail: aldecastro@cbm.uam.es.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
J. J. Cragnolini and J. A. L. de Castro
Identification of Endogenously Presented Peptides from Chlamydia trachomatis with High Homology to Human Proteins and to a Natural Self-ligand of HLA-B27
Mol. Cell. Proteomics, January 1, 2008; 7(1): 170 - 180.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Sesma, I. Alvarez, M. Marcilla, A. Paradela, and J. A. L. de Castro
Species-specific Differences in Proteasomal Processing and Tapasin-mediated Loading Influence Peptide Presentation by HLA-B27 in Murine Cells
J. Biol. Chem., November 21, 2003; 278(47): 46461 - 46472.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Ramos, I. Alvarez, L. Sesma, A. Logean, D. Rognan, and J. A. Lopez de Castro
Molecular Mimicry of an HLA-B27-derived Ligand of Arthritis-linked Subtypes with Chlamydial Proteins
J. Biol. Chem., September 27, 2002; 277(40): 37573 - 37581.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Sesma, V. Montserrat, J. R. Lamas, A. Marina, J. Vazquez, and J. A. L. de Castro
The Peptide Repertoires of HLA-B27 Subtypes Differentially Associated to Spondyloarthropathy (B*2704 and B*2706) Differ by Specific Changes at Three Anchor Positions
J. Biol. Chem., May 3, 2002; 277(19): 16744 - 16749.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Alvarez, M. Marti, J. Vazquez, E. Camafeita, S. Ogueta, and J. A. Lopez de Castro
The Cys-67 Residue of HLA-B27 Influences Cell Surface Stability, Peptide Specificity, and T-cell Antigen Presentation
J. Biol. Chem., December 21, 2001; 276(52): 48740 - 48747.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement