JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M103951200 on July 6, 2001

J. Biol. Chem., Vol. 276, Issue 35, 32854-32859, August 31, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/35/32854    most recent
M103951200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ammanamanchi, S.
Right arrow Articles by Brattain, M. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ammanamanchi, S.
Right arrow Articles by Brattain, M. G.

5-AzaC Treatment Enhances Expression of Transforming Growth Factor-beta Receptors through Down-regulation of Sp3*

Sudhakar Ammanamanchi and Michael G. BrattainDagger

From the Departments of Surgery and Biochemistry, The University of Texas Health Science Center, San Antonio, Texas 78229

We have previously reported that Sp3 acts as a transcriptional repressor of transforming growth factor-beta receptors type I (RI) and type II (RII). We now present data suggesting that treatment of MCF-7L breast and GEO colon cancer cells with 5-aza cytidine (5-azaC) leads to down-regulation of Sp3 and the concomitant induction of RI and RII. Western blot and gel shift analyses on 5-azaC-treated MCF-7L and GEO nuclear extracts indicated reduced Sp3 protein levels and decreased binding of Sp3 protein to radiolabeled consensus Sp1 oligonucleotide. Southwestern analysis detected decreased binding of Sp3 to RI and RII promoters in 5-azaC-treated MCF-7L and GEO cells, suggesting a correlation between decreased Sp3 binding and enhanced RI and RII expression in these cells. Reverse transcription-polymerase chain reaction and nuclear run-on data from 5-azaC-treated MCF-7L and GEO cells indicated down-regulation of Sp3 mRNA as a result of decreased transcription of Sp3. We reported earlier that 5-azaC treatment induces RI and RII expression through increased Sp1 protein levels/activities in these cells. These studies demonstrate that the effect of 5-azaC involves a combination of effects on Sp1 and Sp3.

* This work was supported by National Institutes of Health Grants CA 38173, CA 50457, and CA 72001.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Elm and Carlton Sts., Buffalo, NY 14263. Tel.: 716-845-8224; Fax: 716-845-4437.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 Cancer Res.Home page
R. A. Hinshelwood, L. I. Huschtscha, J. Melki, C. Stirzaker, A. Abdipranoto, B. Vissel, T. Ravasi, C. A. Wells, D. A. Hume, R. R. Reddel, et al.
Concordant Epigenetic Silencing of Transforming Growth Factor- Signaling Pathway Genes Occurs Early in Breast Carcinogenesis
Cancer Res., December 15, 2007; 67(24): 11517 - 11527.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Zhang, N. Fatima, and M. L. Dufau
Coordinated Changes in DNA Methylation and Histone Modifications Regulate Silencing/Derepression of Luteinizing Hormone Receptor Gene Transcription
Mol. Cell. Biol., September 15, 2005; 25(18): 7929 - 7939.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Z. Lou, S. O'Reilly, H. Liang, V. M. Maher, S. D. Sleight, and J. J. McCormick
Down-Regulation of Overexpressed Sp1 Protein in Human Fibrosarcoma Cell Lines Inhibits Tumor Formation
Cancer Res., February 1, 2005; 65(3): 1007 - 1017.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ammanamanchi and M. G. Brattain
Restoration of Transforming Growth Factor-{beta} Signaling through Receptor RI Induction by Histone Deacetylase Activity Inhibition in Breast Cancer Cells
J. Biol. Chem., July 30, 2004; 279(31): 32620 - 32625.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Milutinovic, S. E. Brown, Q. Zhuang, and M. Szyf
DNA Methyltransferase 1 Knock Down Induces Gene Expression by a Mechanism Independent of DNA Methylation and Histone Deacetylation
J. Biol. Chem., July 2, 2004; 279(27): 27915 - 27927.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
R. T.-K. Pang, L. T.-O. Lee, S. S.-M. Ng, W.-H. Yung, and B. K.-C. Chow
CpG Methylation and Transcription Factors Sp1 and Sp3 Regulate the Expression of the Human Secretin Receptor Gene
Mol. Endocrinol., February 1, 2004; 18(2): 471 - 483.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ammanamanchi, J. W. Freeman, and M. G. Brattain
Acetylated Sp3 Is a Transcriptional Activator
J. Biol. Chem., September 12, 2003; 278(37): 35775 - 35780.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Bakker, X. Lin, and W. G. Nelson
Methyl-CpG Binding Domain Protein 2 Represses Transcription from Hypermethylated pi -Class Glutathione S-Transferase Gene Promoters in Hepatocellular Carcinoma Cells
J. Biol. Chem., June 14, 2002; 277(25): 22573 - 22580.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.