HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 35, 33257-33264, August 31, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/35/33257    most recent M104354200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yu, L. Articles by Morse, R. H. Search for Related Content PubMed PubMed Citation Articles by Yu, L. Articles by Morse, R. H. Social Bookmarking                      What's this?

The N-terminal and C-terminal Domains of RAP1 Are Dispensable for Chromatin Opening and GCN4-mediated HIS4 Activation in Budding Yeast^*

Liuning Yu, Nevin Sabet^§, Alistair Chambers^¶, and Randall H. Morse^§

From the  Department of Biomedical Sciences, State University of New York at Albany School of Public Health, Albany, New York 12201-2002, the ^§ Laboratory of Developmental Genetics, Wadsworth Center, New York State Department of Health, Albany, New York 12201-2002, and the ^¶ Division of Genetics, University of Nottingham, Queen's Medical Center, Nottingham NG7 2UH, United Kingdom

Repressor activator protein 1 (RAP1) assists GCN4-mediated HIS4 activation by overcoming some repressive aspect of chromatin structure to facilitate GCN4 binding. RAP1 also participates in other nuclear processes, and discrete domains of RAP1 have been shown to have specific properties including DNA binding, DNA bending, transcriptional activation, and silencing and telomere functions. To investigate whether specific domains of RAP1 are required to "open" chromatin and help GCN4 to activate the HIS4 gene, we examined the abilities of different truncated RAP1 proteins to perturb positioned nucleosomes via a nucleosomal RAP1 site in a yeast episome in vivo, and we tested HIS4 activation in yeast strains harboring truncated RAP1 mutants. We found that neither the DNA bending domain nor the putative activation domain of RAP1 is required for its ability to perturb the chromatin structure of a plasmid containing a RAP1 site. Similarly, neither the putative activation domain nor the N-terminal DNA-bending domain was required for GCN4-mediated activation of HIS4. We also used a rap1^ts mutant to show that continuous occupancy of the HIS4 promoter by RAP1 is required for GCN4-mediated gene activation.

This paper is dedicated to the memory of a good friend, Alan Wolffe.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				A. Yarragudi, T. Miyake, R. Li, and R. H. Morse Comparison of ABF1 and RAP1 in Chromatin Opening and Transactivator Potentiation in the Budding Yeast Saccharomyces cerevisiae Mol. Cell. Biol., October 15, 2004; 24(20): 9152 - 9164. [Abstract] [Full Text] [PDF]

				J. A. Miller and J. Widom Collaborative Competition Mechanism for Gene Activation In Vivo Mol. Cell. Biol., March 1, 2003; 23(5): 1623 - 1632. [Abstract] [Full Text] [PDF]