JBC Ideal method for primary cell transfection

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Originally published In Press as doi:10.1074/jbc.M104978200 on July 3, 2001

J. Biol. Chem., Vol. 276, Issue 36, 33576-33581, September 7, 2001
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Functional Importance of Stat3 Tetramerization in Activation of the alpha 2-Macroglobulin Gene*

Xiaokui ZhangDagger and James E. Darnell Jr.§

From the Laboratory of Molecular Cell Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021

A tetrameric Stat3 complex was found to be essential in transfection experiments for maximal interleukin-6-inducible activation of alpha 2-macroglobulin gene promoter. Stable tetramer formation of purified phosphorylated Stat3 was dependent on protein·protein interaction involving the N-terminal domain of Stat3. The functional importance of tetramer formation was shown by the decreased levels of transcriptional activation associated with hypomorphic mutations in N-terminal residues.

* This work was supported by Grants AI32489 and AI34420 from the National Institutes of Health (to J. E. D.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Supported by Training Grant CA09673 from the National Institutes of Health.

§ To whom correspondence should be addressed. Tel.: 212-327-8791; Fax: 212-327-8801; E-mail: darnell@rockvax.rockefeller.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


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Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.