| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 36, 33840-33846, September 7, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
,
From the Endocrinology and Reproduction Research Branch, NICHD,
National Institutes of Health, Bethesda, Maryland 20892-4510
In excitable cells, voltage-gated calcium
influx provides an effective mechanism for the activation of
exocytosis. In this study, we demonstrate that although rat anterior
pituitary lactotrophs, somatotrophs, and gonadotrophs exhibited
spontaneous and extracellular calcium-dependent
electrical activity, voltage-gated calcium influx triggered secretion
only in lactotrophs and somatotrophs. The lack of action
potential-driven secretion in gonadotrophs was not due to the
proportion of spontaneously firing cells or spike frequency.
Gonadotrophs exhibited calcium signals during prolonged depolarization
comparable with signals observed in somatotrophs and
lactotrophs. The secretory vesicles in all three cell types also
had a similar sensitivity to voltage-gated calcium influx. However, the
pattern of action potential calcium influx differed among three cell
types. Spontaneous activity in gonadotrophs was characterized by high
amplitude, sharp spikes that had a limited capacity to promote calcium
influx, whereas lactotrophs and somatotrophs fired plateau-bursting
action potentials that generated high amplitude calcium signals.
Furthermore, a shift in the pattern of firing from sharp spikes to
plateau-like spikes in gonadotrophs triggered luteinizing
hormone secretion. These results indicate that the cell
type-specific action potential secretion coupling in pituitary cells is
determined by the capacity of their plasma membrane oscillator to
generate threshold calcium signals.
Present address: Aurora Biosciences Corporation, San Diego, CA 92121.
§
To whom correspondence should be addressed: Section on Cellular
Signaling, ERRB/NICHD, Bldg. 49, Rm. 6A-36, 49 Convent Dr., Bethesda,
MD 20892-4510. Tel.: 301-496-1638; Fax: 301-594-7031; E-mail:
stankos@helix.nih.gov.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. E. Gonzalez-Iglesias, T. Murano, S. Li, M. Tomic, and S. S. Stojilkovic Dopamine Inhibits Basal Prolactin Release in Pituitary Lactotrophs through Pertussis Toxin-Sensitive and -Insensitive Signaling Pathways Endocrinology, April 1, 2008; 149(4): 1470 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Toporikova, J. Tabak, M. E. Freeman, and R. Bertram A-Type K+ Current Can Act as a Trigger for Bursting in the Absence of a Slow Variable Neural Comput., February 1, 2008; 20(2): 436 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tsaneva-Atanasova, A. Sherman, F. van Goor, and S. S. Stojilkovic Mechanism of Spontaneous and Receptor-Controlled Electrical Activity in Pituitary Somatotrophs: Experiments and Theory J Neurophysiol, July 1, 2007; 98(1): 131 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Gonzalez-Iglesias, Y. Jiang, M. Tomic, K. Kretschmannova, S. A. Andric, H. Zemkova, and S. S. Stojilkovic Dependence of Electrical Activity and Calcium Influx-Controlled Prolactin Release on Adenylyl Cyclase Signaling Pathway in Pituitary Lactotrophs Mol. Endocrinol., September 1, 2006; 20(9): 2231 - 2246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zemkova, A. Balik, Y. Jiang, K. Kretschmannova, and S. S. Stojilkovic Roles of Purinergic P2X Receptors as Pacemaking Channels and Modulators of Calcium-Mobilizing Pathway in Pituitary Gonadotrophs Mol. Endocrinol., June 1, 2006; 20(6): 1423 - 1436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ben-Tabou De-Leon, G. Ben-Zeev, and I. Nussinovitch Effects of osmotic shrinkage on voltage-gated Ca2+ channel currents in rat anterior pituitary cells Am J Physiol Cell Physiol, January 1, 2006; 290(1): C222 - C232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Andric, D. Zivadinovic, A. E. Gonzalez-Iglesias, A. Lachowicz, M. Tomic, and S. S. Stojilkovic Endothelin-induced, Long Lasting, and Ca2+ Influx-independent Blockade of Intrinsic Secretion in Pituitary Cells by Gz Subunits J. Biol. Chem., July 22, 2005; 280(29): 26896 - 26903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Bergh, Y. Xu, and M. C. Farach-Carson Osteoprotegerin Expression and Secretion Are Regulated by Calcium Influx through the L-Type Voltage-Sensitive Calcium Channel Endocrinology, January 1, 2004; 145(1): 426 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ben-Tabou De-Leon, E. Blotnick, and I. Nussinovitch Effects of osmotic swelling on voltage-gated calcium channel currents in rat anterior pituitary cells Am J Physiol Cell Physiol, October 1, 2003; 285(4): C840 - C852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Andric, A. E. Gonzalez-Iglesias, F. Van Goor, M. Tomic, and S. S. Stojilkovic Nitric Oxide Inhibits Prolactin Secretion in Pituitary Cells Downstream of Voltage-Gated Calcium Influx Endocrinology, July 1, 2003; 144(7): 2912 - 2921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Dumont, S. Dremier, I. Pirson, and C. Maenhaut Cross signaling, cell specificity, and physiology Am J Physiol Cell Physiol, July 1, 2002; 283(1): C2 - C28. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tomic', F. Van Goor, M.-L. He, D. Zivadinovic, and S. S. Stojilkovic Ca2+-Mobilizing Endothelin-A Receptors Inhibit Voltage-Gated Ca2+ Influx through Gi/o Signaling Pathway in Pituitary Lactotrophs Mol. Pharmacol., June 1, 2002; 61(6): 1329 - 1339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zivadinovic, M. Tomic, D. Yuan, and S. S. Stojilkovic Cell-Type Specific Messenger Functions of Extracellular Calcium in the Anterior Pituitary Endocrinology, February 1, 2002; 143(2): 445 - 455. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
