HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 37, 34784-34791, September 14, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/37/34784    most recent M011633200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schmidt, S. L. G. Articles by Burgers, P. M. J. Search for Related Content PubMed PubMed Citation Articles by Schmidt, S. L. G. Articles by Burgers, P. M. J.

ATP Utilization by Yeast Replication Factor C
III. THE ATP-BINDING DOMAINS OF Rfc2, Rfc3, AND Rfc4 ARE ESSENTIAL FOR DNA RECOGNITION AND CLAMP LOADING^*

Sonja L. Gary Schmidt, Xavier V. Gomes, and Peter M. J. Burgers

From the Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110

The conserved lysine in the Walker A motif of the ATP-binding domain encoded by the yeast RFC1, RFC2, RFC3, and RFC4 genes was mutated to glutamic acid. Complexes of replication factor C with a N-terminal truncation (2-273) of the Rfc1 subunit (RFC) containing a single mutant subunit were overproduced in Escherichia coli for biochemical analysis. All of the mutant RFC complexes were capable of interacting with PCNA. Complexes containing a rfc1-K359E mutation were similar to wild type in replication activity and ATPase activity; however, the mutant complex showed increased susceptibility to proteolysis. In contrast, complexes containing either a rfc2-K71E mutation or a rfc3-K59E mutation were severely impaired in ATPase and clamp loading activity. In addition to their defects in ATP hydrolysis, these complexes were defective for DNA binding. A mutant complex containing the rfc4-K55E mutation performed as well as a wild type complex in clamp loading, but only at very high ATP concentrations. Mutant RFC complexes containing rfc2-K71R or rfc3-K59R, carrying a conservative lysine  arginine mutation, had much milder clamp loading defects that could be partially (rfc2-K71R) or completely (rfc3-K59R) suppressed at high ATP concentrations.

This article has been cited by other articles:

				A. Johnson, N. Y. Yao, G. D. Bowman, J. Kuriyan, and M. O'Donnell The Replication Factor C Clamp Loader Requires Arginine Finger Sensors to Drive DNA Binding and Proliferating Cell Nuclear Antigen Loading J. Biol. Chem., November 17, 2006; 281(46): 35531 - 35543. [Abstract] [Full Text] [PDF]

				J. Majka, S. K. Binz, M. S. Wold, and P. M. J. Burgers Replication Protein A Directs Loading of the DNA Damage Checkpoint Clamp to 5'-DNA Junctions J. Biol. Chem., September 22, 2006; 281(38): 27855 - 27861. [Abstract] [Full Text] [PDF]

				G. O. Bylund and P. M. J. Burgers Replication Protein A-Directed Unloading of PCNA by the Ctf18 Cohesion Establishment Complex Mol. Cell. Biol., July 1, 2005; 25(13): 5445 - 5455. [Abstract] [Full Text] [PDF]

				J. Majka, B. Y. Chung, and P. M. J. Burgers Requirement for ATP by the DNA Damage Checkpoint Clamp Loader J. Biol. Chem., May 14, 2004; 279(20): 20921 - 20926. [Abstract] [Full Text] [PDF]

				S. L. Reck-Peterson and R. D. Vale Molecular dissection of the roles of nucleotide binding and hydrolysis in dynein's AAA domains in Saccharomyces cerevisiae PNAS, February 10, 2004; 101(6): 1491 - 1495. [Abstract] [Full Text] [PDF]

				N. Yao, L. Coryell, D. Zhang, R. E. Georgescu, J. Finkelstein, M. M. Coman, M. M. Hingorani, and M. O'Donnell Replication Factor C Clamp Loader Subunit Arrangement within the Circular Pentamer and Its Attachment Points to Proliferating Cell Nuclear Antigen J. Biol. Chem., December 12, 2003; 278(50): 50744 - 50753. [Abstract] [Full Text] [PDF]

				M. A. Kenna and R. V. Skibbens Mechanical Link between Cohesion Establishment and DNA Replication: Ctf7p/Eco1p, a Cohesion Establishment Factor, Associates with Three Different Replication Factor C Complexes Mol. Cell. Biol., April 15, 2003; 23(8): 2999 - 3007. [Abstract] [Full Text] [PDF]

				A. Johnson and M. O'Donnell Ordered ATP Hydrolysis in the gamma Complex Clamp Loader AAA+ Machine J. Biol. Chem., April 11, 2003; 278(16): 14406 - 14413. [Abstract] [Full Text] [PDF]

				M. M. Hingorani and M. M. Coman On the Specificity of Interaction between the Saccharomyces cerevisiae Clamp Loader Replication Factor C and Primed DNA Templates during DNA Replication J. Biol. Chem., November 27, 2002; 277(49): 47213 - 47224. [Abstract] [Full Text] [PDF]

				R. Dua, D. L. Levy, C. M. Li, P. M. Snow, and J. L. Campbell In Vivo Reconstitution of Saccharomyces cerevisiae DNA Polymerase epsilon in Insect Cells. PURIFICATION AND CHARACTERIZATION J. Biol. Chem., March 1, 2002; 277(10): 7889 - 7896. [Abstract] [Full Text] [PDF]

				X. V. Gomes and P. M. J. Burgers ATP Utilization by Yeast Replication Factor C. I. ATP-MEDIATED INTERACTION WITH DNA AND WITH PROLIFERATING CELL NUCLEAR ANTIGEN J. Biol. Chem., September 7, 2001; 276(37): 34768 - 34775. [Abstract] [Full Text] [PDF]

				S. L. G. Schmidt, A. L. Pautz, and P. M. J. Burgers ATP Utilization by Yeast Replication Factor C. IV. RFC ATP-BINDING MUTANTS SHOW DEFECTS IN DNA REPLICATION, DNA REPAIR, AND CHECKPOINT REGULATION J. Biol. Chem., September 7, 2001; 276(37): 34792 - 34800. [Abstract] [Full Text] [PDF]