Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M102063200 on June 15, 2001

J. Biol. Chem., Vol. 276, Issue 37, 34888-34895, September 14, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/37/34888    most recent
M102063200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gyles, S. L.
Right arrow Articles by Jones, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gyles, S. L.
Right arrow Articles by Jones, P. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

ERKs Regulate Cyclic AMP-induced Steroid Synthesis through Transcription of the Steroidogenic Acute Regulatory (StAR) Gene*

Shân L. GylesDagger §, Chris J. BurnsDagger §, Barbara J. WhitehouseDagger , David SugdenDagger , Phil J. Marsh||, Shanta J. PersaudDagger , and Peter M. JonesDagger **

From the Dagger  Endocrinology and Reproduction Research Group and The || Randall Centre, Guy's, King's and St. Thomas's School of Biomedical Sciences, King's College London, London SE1 1UL, United Kingdom

Cyclic AMP-dependent expression of the steroidogenic acute regulatory (StAR) protein is thought to be the controlling step for steroid production, but the mechanisms through which external signals are translated into increased transcription of the StAR gene are unknown. We demonstrate that cyclic AMP-induced steroid synthesis is dependent upon the phosphorylation and activation of ERKs and that ERK activation results in enhanced phosphorylation of SF-1 and increased steroid production through increased transcription of the StAR gene. Adenylate cyclase activation with forskolin (FSK) caused a time-dependent increase in ERK activity and translocation from cytoplasm to nucleus, which correlated with an increase in StAR mRNA levels, StAR protein accumulation, and steroidogenesis. Similarly, ERK inhibition led to a reduction in the levels of FSK-stimulated StAR mRNA, StAR protein, and steroid secretion. These effects were attributed to the finding that ERK activity is required for SF-1 phosphorylation, a transcription factor required for the regulation of StAR gene transcription. This conclusion was supported by our demonstration of an ERK-dependent increase in the binding of SF-1 from FSK-treated Y1 nuclei to three consensus double-stranded DNA sequences from the StAR promoter region. These observations suggest that the activation of ERK2/1 by increasing cAMP is an obligatory and regulated stage in the stimulation of steroid synthesis by cyclic AMP-generating stimuli.

* This work was supported by Wellcome Trust Grant 054789/Z/98/Z and a research and development grant from the Guy's and St. Thomas's Charitable Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ These authors share first authorship of this study.

A Biotechnology and Biological Sciences Research Council postgraduate student.

** To whom correspondence should be addressed: Endocrinology and Reproduction Research Group, New Hunt's House, 3rd Fl. North, GKT School of Biomedical Sciences, King's College London, London SE1 1UL, UK. Tel.: 44-207-848-6273; Fax: 44-207-848-6280; E-mail: peter.jones@kcl.ac.uk.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 EndocrinologyHome page
P. R. Manna, I. T. Huhtaniemi, and D. M. Stocco
Mechanisms of Protein Kinase C Signaling in the Modulation of 3',5'-Cyclic Adenosine Monophosphate-Mediated Steroidogenesis in Mouse Gonadal Cells
Endocrinology, July 1, 2009; 150(7): 3308 - 3317.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. R. Manna, M. T. Dyson, Y. Jo, and D. M. Stocco
Role of Dosage-Sensitive Sex Reversal, Adrenal Hypoplasia Congenita, Critical Region on the X Chromosome, Gene 1 in Protein Kinase A- and Protein Kinase C-Mediated Regulation of the Steroidogenic Acute Regulatory Protein Expression in Mouse Leydig Tumor Cells: Mechanism of Action
Endocrinology, January 1, 2009; 150(1): 187 - 199.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
L. J. Martin, N. Boucher, C. Brousseau, and J. J. Tremblay
The Orphan Nuclear Receptor NUR77 Regulates Hormone-Induced StAR Transcription in Leydig Cells through Cooperation with Ca2+/Calmodulin-Dependent Protein Kinase I
Mol. Endocrinol., September 1, 2008; 22(9): 2021 - 2037.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. T Dyson, J. K Jones, M. P Kowalewski, P. R Manna, M. Alonso, M. E Gottesman, and D. M Stocco
Mitochondrial A-Kinase Anchoring Protein 121 Binds Type II Protein Kinase A and Enhances Steroidogenic Acute Regulatory Protein-Mediated Steroidogenesis in MA-10 Mouse Leydig Tumor Cells
Biol Reprod, February 1, 2008; 78(2): 267 - 277.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. E. Lewis, M. Rusten, E. A. Hoivik, E. L. Vikse, M. L. Hansson, A. E. Wallberg, and M. Bakke
Phosphorylation of Steroidogenic Factor 1 Is Mediated by Cyclin-Dependent Kinase 7
Mol. Endocrinol., January 1, 2008; 22(1): 91 - 104.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. J. Casal, S. Ryser, A. M. Capponi, and C. F. Wang-Buholzer
Angiotensin II-Induced Mitogen-Activated Protein Kinase Phosphatase-1 Expression in Bovine Adrenal Glomerulosa Cells: Implications in Mineralocorticoid Biosynthesis
Endocrinology, November 1, 2007; 148(11): 5573 - 5581.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. C. Woods, M. J. Haugen, and A.L. Johnson
Actions of Epidermal Growth Factor Receptor/Mitogen-Activated Protein Kinase and Protein Kinase C Signaling in Granulosa Cells from Gallus gallus Are Dependent upon Stage of Differentiation
Biol Reprod, July 1, 2007; 77(1): 61 - 70.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G.-S. Hwang, S.-W. Wang, W.-M. Tseng, C.-H. Yu, and P. S. Wang
Effect of hypoxia on the release of vascular endothelial growth factor and testosterone in mouse TM3 Leydig cells
Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1763 - E1769.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
P. R Manna, Y. Jo, and D. M Stocco
Regulation of Leydig cell steroidogenesis by extracellular signal-regulated kinase 1/2: role of protein kinase A and protein kinase C signaling
J. Endocrinol., April 1, 2007; 193(1): 53 - 63.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
J. G Ferreira, C. D Cruz, D. Neves, and D. Pignatelli
Increased extracellular signal regulated kinases phosphorylation in the adrenal gland in response to chronic ACTH treatment
J. Endocrinol., March 1, 2007; 192(3): 647 - 658.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
E. Gray, D. Muller, P. E Squires, H. Asare-Anane, G.-C. Huang, S. Amiel, S. J Persaud, and P. M Jones
Activation of the extracellular calcium-sensing receptor initiates insulin secretion from human islets of Langerhans: involvement of protein kinases.
J. Endocrinol., September 1, 2006; 190(3): 703 - 710.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
P. R Manna, S. P Chandrala, Y. Jo, and D. M Stocco
cAMP-independent signaling regulates steroidogenesis in mouse Leydig cells in the absence of StAR phosphorylation.
J. Mol. Endocrinol., August 1, 2006; 37(1): 81 - 95.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
N Renlund, Y Jo, I Svechnikova, M Holst, D M Stocco, O Soder, and K Svechnikov
Induction of steroidogenesis in immature rat Leydig cells by interleukin-1alpha is dependent on extracellular signal-regulated kinases.
J. Mol. Endocrinol., April 1, 2006; 36(2): 327 - 336.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. Ragazzon, A.-M. Lefrancois-Martinez, P. Val, I. Sahut-Barnola, C. Tournaire, C. Chambon, J.-L. Gachancard-Bouya, R.-J. Begue, G. Veyssiere, and A. Martinez
Adrenocorticotropin-Dependent Changes in SF-1/DAX-1 Ratio Influence Steroidogenic Genes Expression in a Novel Model of Glucocorticoid-Producing Adrenocortical Cell Lines Derived from Targeted Tumorigenesis
Endocrinology, April 1, 2006; 147(4): 1805 - 1818.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
P. R. Manna, S. P. Chandrala, S. R. King, Y. Jo, R. Counis, I. T. Huhtaniemi, and D. M. Stocco
Molecular Mechanisms of Insulin-like Growth Factor-I Mediated Regulation of the Steroidogenic Acute Regulatory Protein in Mouse Leydig Cells
Mol. Endocrinol., February 1, 2006; 20(2): 362 - 378.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. N. Winnay and G. D. Hammer
Adrenocorticotropic Hormone-Mediated Signaling Cascades Coordinate a Cyclic Pattern of Steroidogenic Factor 1-Dependent Transcriptional Activation
Mol. Endocrinol., January 1, 2006; 20(1): 147 - 166.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
D. M. Stocco, X. Wang, Y. Jo, and P. R. Manna
Multiple Signaling Pathways Regulating Steroidogenesis and Steroidogenic Acute Regulatory Protein Expression: More Complicated than We Thought
Mol. Endocrinol., November 1, 2005; 19(11): 2647 - 2659.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
F.-Q. Yu, C.-S. Han, W. Yang, X. Jin, Z.-Y. Hu, and Y.-X. Liu
Activation of the p38 MAPK pathway by follicle-stimulating hormone regulates steroidogenesis in granulosa cells differentially
J. Endocrinol., July 1, 2005; 186(1): 85 - 96.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
V. L. Nelson-Degrave, J. K. Wickenheisser, K. L. Hendricks, T. Asano, M. Fujishiro, R. S. Legro, S. R. Kimball, J. F. Strauss III, and J. M. McAllister
Alterations in Mitogen-Activated Protein Kinase Kinase and Extracellular Regulated Kinase Signaling in Theca Cells Contribute to Excessive Androgen Production in Polycystic Ovary Syndrome
Mol. Endocrinol., February 1, 2005; 19(2): 379 - 390.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
E C Chin and D R E Abayasekara
Progesterone secretion by luteinizing human granulosa cells: a possible cAMP-dependent but PKA-independent mechanism involved in its regulation
J. Endocrinol., October 1, 2004; 183(1): 51 - 60.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. Martinelle, M. Holst, O. Soder, and K. Svechnikov
Extracellular Signal-Regulated Kinases Are Involved in the Acute Activation of Steroidogenesis in Immature Rat Leydig Cells by Human Chorionic Gonadotropin
Endocrinology, October 1, 2004; 145(10): 4629 - 4634.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. A. LaVoie, D. Singh, and Y. Y. Hui
Concerted Regulation of the Porcine Steroidogenic Acute Regulatory Protein Gene Promoter Activity by Follicle-Stimulating Hormone and Insulin-Like Growth Factor I in Granulosa Cells Involves GATA-4 and CCAAT/Enhancer Binding Protein {beta}
Endocrinology, July 1, 2004; 145(7): 3122 - 3134.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. P. Houk, E. J. Pearson, N. Martinelle, P. K. Donahoe, and J. Teixeira
Feedback Inhibition of Steroidogenic Acute Regulatory Protein Expression in Vitro and in Vivo by Androgens
Endocrinology, March 1, 2004; 145(3): 1269 - 1275.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. Val, C. Aigueperse, B. Ragazzon, G. Veyssiere, A.-M. Lefrancois-Martinez, and A. Martinez
Adrenocorticotropin/3',5'-Cyclic AMP-Mediated Transcription of the Scavenger akr1-b7 Gene in Adrenocortical Cells Is Dependent on Three Functionally Distinct Steroidogenic Factor-1-Responsive Elements
Endocrinology, February 1, 2004; 145(2): 508 - 518.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Li, R. E. Feltzer, K. L. Dawson, E. A. Hudson, and B. J. Clark
Janus Kinase 2 and Calcium Are Required for Angiotensin II-dependent Activation of Steroidogenic Acute Regulatory Protein Transcription in H295R Human Adrenocortical Cells
J. Biol. Chem., December 26, 2003; 278(52): 52355 - 52362.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
R. C. Fowkes, M. Desclozeaux, M. V. Patel, S. J. B. Aylwin, P. King, H. A. Ingraham, and J. M. Burrin
Steroidogenic Factor-1 and The Gonadotrope-Specific Element Enhance Basal and Pituitary Adenylate Cyclase-Activating Polypeptide-Stimulated Transcription of the Human Glycoprotein Hormone {alpha}-Subunit Gene in Gonadotropes
Mol. Endocrinol., November 1, 2003; 17(11): 2177 - 2188.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. S. Sun, K.-Y. Hsiao, C.-C. Hsu, M.-H. Wu, and S.-J. Tsai
Transactivation of Steroidogenic Acute Regulatory Protein in Human Endometriotic Stromal Cells Is Mediated by the Prostaglandin EP2 Receptor
Endocrinology, September 1, 2003; 144(9): 3934 - 3942.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
K. Tajima, A. Dantes, Z. Yao, K. Sorokina, F. Kotsuji, R. Seger, and A. Amsterdam
Down-Regulation of Steroidogenic Response to Gonadotropins in Human and Rat Preovulatory Granulosa Cells Involves Mitogen-Activated Protein Kinase Activation and Modulation of DAX-1 and Steroidogenic Factor-1
J. Clin. Endocrinol. Metab., May 1, 2003; 88(5): 2288 - 2299.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. J. Casal, J.-S. Silvestre, C. Delcayre, and A. M. Capponi
Expression and Modulation of Steroidogenic Acute Regulatory Protein Messenger Ribonucleic Acid in Rat Cardiocytes and after Myocardial Infarction
Endocrinology, May 1, 2003; 144(5): 1861 - 1868.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. R. O. Carvalho, J. B. C. Carvalheira, M. H. M. Lima, S. F. Zimmerman, L. C. Caperuto, A. Amanso, A. L. Gasparetti, V. Meneghetti, L. F. Zimmerman, L. A. Velloso, et al.
Novel Signal Transduction Pathway for Luteinizing Hormone and Its Interaction with Insulin: Activation of Janus Kinase/Signal Transducer and Activator of Transcription and Phosphoinositol 3-Kinase/Akt Pathways
Endocrinology, February 1, 2003; 144(2): 638 - 647.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P. R. Manna, I. T. Huhtaniemi, X.-J. Wang, D. W. Eubank, and D. M. Stocco
Mechanisms of Epidermal Growth Factor Signaling: Regulation of Steroid Biosynthesis and the Steroidogenic Acute Regulatory Protein in Mouse Leydig Tumor Cells
Biol Reprod, November 1, 2002; 67(5): 1393 - 1404.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Takemori, Y. Katoh, N. Horike, J. Doi, and M. Okamoto
ACTH-induced Nucleocytoplasmic Translocation of Salt-inducible Kinase. IMPLICATION IN THE PROTEIN KINASE A-ACTIVATED GENE TRANSCRIPTION IN MOUSE ADRENOCORTICAL TUMOR CELLS
J. Biol. Chem., October 25, 2002; 277(44): 42334 - 42343.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Osman, C. Murigande, A. Nadakal, and A. M. Capponi
Repression of DAX-1 and Induction of SF-1 Expression. TWO MECHANISMS CONTRIBUTING TO THE ACTIVATION OF ALDOSTERONE BIOSYNTHESIS IN ADRENAL GLOMERULOSA CELLS
J. Biol. Chem., October 18, 2002; 277(43): 41259 - 41267.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. J. Tremblay, F. Hamel, and R. S. Viger
Protein Kinase A-Dependent Cooperation between GATA and CCAAT/Enhancer-Binding Protein Transcription Factors Regulates Steroidogenic Acute Regulatory Protein Promoter Activity
Endocrinology, October 1, 2002; 143(10): 3935 - 3945.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
V. M. Laurich, A. M. Trbovich, F. H. O'Neill, C. P. Houk, P. M. Sluss, A. H. Payne, P. K. Donahoe, and J. Teixeira
Mullerian Inhibiting Substance Blocks the Protein Kinase A-Induced Expression of Cytochrome P450 17{alpha}-Hydroxylase/C17-20 Lyase mRNA in a Mouse Leydig Cell Line Independent of cAMP Responsive Element Binding Protein Phosphorylation
Endocrinology, September 1, 2002; 143(9): 3351 - 3360.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. C. Fowkes, P. King, and J. M. Burrin
Regulation of Human Glycoprotein Hormone {alpha}-Subunit Gene Transcription in L{beta}T2 Gonadotropes by Protein Kinase C and Extracellular Signal-Regulated Kinase 1/2
Biol Reprod, September 1, 2002; 67(3): 725 - 734.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. A. Dewi, D. R. E. Abayasekara, and C. P. D. Wheeler-Jones
Requirement for ERK1/2 Activation in the Regulation of Progesterone Production in Human Granulosa-Lutein Cells Is Stimulus Specific
Endocrinology, March 1, 2002; 143(3): 877 - 888.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement