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Originally published In Press as doi:10.1074/jbc.M104938200 on July 13, 2001

J. Biol. Chem., Vol. 276, Issue 37, 35201-35208, September 14, 2001
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Homologous Pairing Promoted by the Human Rad52 Protein*

Wataru KagawaDagger §, Hitoshi KurumizakaDagger , Shukuko Ikawa||, Shigeyuki YokoyamaDagger §**, and Takehiko Shibata||Dagger Dagger

From the Dagger  RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan, the  Cellular Signaling Laboratory, RIKEN Harima Institute at Spring-8, 1-1-1 Kohto, Mikazuki-cho, Sayo, Hyogo 679-5143, Japan, the || Cellular and Molecular Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan, and the § Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

The Rad52 protein, which is unique to eukaryotes, plays important roles in the Rad51-dependent and the Rad51-independent pathways of DNA recombination. In the present study, we have biochemically characterized the homologous pairing activity of the HsRad52 protein (Homo sapiens Rad52) and found that the presynaptic complex formation with ssDNA is essential in its catalysis of homologous pairing. We have identified an N-terminal fragment (amino acid residues 1-237, HsRad521-237) that is defective in binding to the human Rad51 protein, which catalyzed homologous pairing as efficiently as the wild type HsRad52. Electron microscopic visualization revealed that HsRad52 and HsRad521-237 both formed nucleoprotein filaments with single-stranded DNA. These lines of evidence suggest the role of HsRad52 in the homologous pairing step of the Rad51-independent recombination pathway. Our results reveal the striking similarity between HsRad52 and the Escherichia coli RecT protein, which functions in a RecA-independent recombination pathway.

* This work was supported by the Bioarchitect Research Program (RIKEN), CREST of Japan Science and Technology, and by a Grant-in-Aid from the Ministry of Education, Sports, Culture, Science, and Technology of Japan.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

** To whom correspondence may be addressed.

Dagger Dagger To whom correspondence may be addressed.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


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