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Originally published In Press as doi:10.1074/jbc.C100304200 on July 25, 2001

J. Biol. Chem., Vol. 276, Issue 38, 35227-35230, September 21, 2001
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ACCELERATED PUBLICATION
Strong Growth Polarity of Yeast Prion Fiber Revealed by Single Fiber Imaging*

Yuji InoueDagger , Aiko KishimotoDagger , Jun Hirao, Masasuke Yoshida§, and Hideki Taguchi

From the Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226-8503, Japan

Using the yeast prion as a model, we have developed a novel system to observe the growth of individual prion fibers directly. NM fragments, the prion-determining region of the yeast protein Sup35p, were labeled by either red or green fluorescent dyes, and the fiber growth was observed under a fluorescence microscope. When green-Sup35NM was added to the preformed fibers made of red-Sup35NM, 70-97% of green fibers grew unidirectionally, from only one end of individual red fibers, whereas the remainder grew from both ends. Similarly, the majority of red fibers grew from only one end of green fibers when the order of addition was reversed. Sonication of preformed fibers to expose fresh ends did not change the results, excluding a possibility of occasional deformation of one end as the reason of the apparent unidirectional growth. These results indicate the polarity of Sup35 prion fibers and impose constraints on the models of fiber growth.

* This work was supported by a grant-in-aid for Scientific Research on Priority Areas (A) from the Ministry of Education, Science, Sports and Culture of Japan.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Contributed equally to this work.

§ To whom correspondence should be addressed: Chemical Resources Laboratory, R-1, Tokyo Inst. of Technology, 4259 Nagatsuta, Yokohama 226-8503, Japan. Tel.: 81-45-924-5233; Fax: 81-45-924-5277; E-mail: myoshida@res.titech.ac.jp.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


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