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Originally published In Press as doi:10.1074/jbc.C100365200 on July 31, 2001

J. Biol. Chem., Vol. 276, Issue 38, 35231-35234, September 21, 2001
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ACCELERATED PUBLICATION
Three Molecules of Ubiquinone Bind Specifically to Mitochondrial Cytochrome bc1 Complex*,

Stefan BartoschekDagger , Malin Johansson§, Bernhard H. GeierstangerDagger , Jürgen G. Okun§, C. Roy D. Lancaster, Eberhard Humpfer||, Linda Yu**, Chang-An Yu**Dagger Dagger , Christian GriesingerDagger Dagger Dagger , and Ulrich Brandt§Dagger Dagger

From the Dagger  Johann Wolfgang Goethe-Universität Frankfurt, Institut für Organische Chemie, Marie-Curie-Strasse 11, D-60439 Frankfurt am Main, Germany, the Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany, the § Universitätsklinikum Frankfurt, Institut für Biochemie I, D-60590 Frankfurt am Main, Germany, the  Max-Planck-Institut für Biophysik, Abteilung für Molekulare Membranbiologie, Heinrich-Hoffmann-Strasse 7, D-60528 Frankfurt am Main, Germany, || Bruker Analytik GmbH, Silberstreifen 4, D-76287 Rheinstetten, Germany, and the ** Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078

Bifurcated electron flow to high potential "Rieske" iron-sulfur cluster and low potential heme bL is crucial for respiratory energy conservation by the cytochrome bc1 complex. The chemistry of ubiquinol oxidation has to ensure the thermodynamically unfavorable electron transfer to heme bL. To resolve a central controversy about the number of ubiquinol molecules involved in this reaction, we used high resolution magic-angle-spinning nuclear magnetic resonance experiments to show that two out of three n-decyl-ubiquinones bind at the ubiquinol oxidation center of the complex. This substantiates a proposed mechanism in which a charge transfer between a ubiquinol/ubiquinone pair explains the bifurcation of electron flow.

* This work was supported by Deutsche Forschungsgemeinschaft Grant SFB 472 and Graduiertenkolleg Grant 145/12, National Institutes of Health Grant GM30721, the Max-Planck Society, the Bundesministerium für Bildung und Forschung, and the Fonds der Chemischen Industrie.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (http://www.jbc.org) contains a table.

Dagger Dagger To whom correspondence may be addressed. Tel.: 49-69/63016926, Fax: 49-69/63016970; E-mail: brandt@zbc.klinik.uni-frankfurt.de (for U. B.); Tel.: 49-69/79829130, Fax: 49-69/79829128, E-mail: cigr@org.chemie.uni-frankfurt.de (for C. G.); Tel.: 405-744-6612, Fax: 405-744-7799, E-mail: cayuq@okstate.edu (for C.-A. Y.).

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


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