|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 38, 35581-35588, September 21, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Biochemistry, University of Medicine and
Dentistry of New Jersey-Robert Wood Johnson Medical School,
Piscataway, New Jersey 08854
CspA, the major cold shock protein of
Escherichia coli, is dramatically induced immediately after
cold shock. CspA production is transient and reduces to a low basal
level when cells become adapted. Here we show that expression from
multicopy plasmids of mutant cspA mRNAs bearing
nonsense mutations in the coding region caused sustained high levels of
the mutant mRNAs at low temperature, resulting in complete
inhibition of cell growth ultimately leading to cell death. We
demonstrate that the observed growth inhibition was caused by largely
exclusive occupation of cellular ribosomes by the mutant
cspA mRNAs. Such sequestration of ribosomes even occurs
without a single peptide bond formation, implying that the robust
translatability of the cspA mRNA is determined at the
step of initiation. Further analysis demonstrated that the downstream
box of the cspA mRNA was dispensable for the
effect, whereas the upstream box of the mRNA was essential. Our
system may offer a novel means to study sequence or structural elements involved in the translation of the cspA mRNA and may
also be utilized to regulate bacterial growth at low temperature.
Nonsense Mutations in cspA Cause Ribosome Trapping
Leading to Complete Growth Inhibition and Cell Death at Low Temperature
in Escherichia coli*
, and
*
This work was supported by Grant GM19043 from the National
Institutes of Health.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Lab. of Developmental Chronobiology, Pediatric
Service, Massachusetts General Hospital/Harvard Medical School, 32 Fruit St., GRJ 1226, Boston, MA 02114.
§
To whom correspondence should be addressed: Dept. of Biochemistry,
UMDNJ-Robert Wood Johnson Medical School, 675 Hoes Ln., Piscataway, NJ
08854. Tel.: 732-235-4115; Fax: 732-235-4559; E-mail: inouye@umdnj.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Y. Lee, B. H. Sung, B. J. Yu, J. H. Lee, S. H. Lee, M. S. Kim, M. D. Koob, and S. C. Kim Phenotypic engineering by reprogramming gene transcription using novel artificial transcription factors in Escherichia coli Nucleic Acids Res., September 1, 2008; 36(16): e102 - e102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zeeb, K. E.A. Max, U. Weininger, C. Low, H. Sticht, and J. Balbach Recognition of T-rich single-stranded DNA by the cold shock protein Bs-CspB in solution Nucleic Acids Res., September 11, 2006; 34(16): 4561 - 4571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Derzelle, B. Hallet, T. Ferain, J. Delcour, and P. Hols Cold Shock Induction of the cspL Gene in Lactobacillus plantarum Involves Transcriptional Regulation J. Bacteriol., October 1, 2002; 184(19): 5518 - 5523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Xia, H. Ke, W. Jiang, and M. Inouye The Cold Box Stem-loop Proximal to the 5'-End of the Escherichia coli cspA Gene Stabilizes Its mRNA at Low Temperature J. Biol. Chem., February 15, 2002; 277(8): 6005 - 6011. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |