Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M105472200 on July 13, 2001

J. Biol. Chem., Vol. 276, Issue 38, 36051-36057, September 21, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/38/36051    most recent
M105472200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Randerath, K.
Right arrow Articles by Brooks, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Randerath, K.
Right arrow Articles by Brooks, P. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

A 32P-Postlabeling Assay for the Oxidative DNA Lesion 8,5'-Cyclo-2'-deoxyadenosine in Mammalian Tissues
EVIDENCE THAT FOUR TYPE II I-COMPOUNDS ARE DINUCLEOTIDES CONTAINING THE LESION IN THE 3' NUCLEOTIDE*

Kurt RanderathDagger , Guo-Dong ZhouDagger , Robert L. Somers§, Jay H. Robbins, and Philip J. Brooks||**

From the Dagger  Division of Toxicology, Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, the § Glen Research Corporation, Sterling, Virginia 20164, and the  Dermatology Branch, NCI and || Laboratory of Neurogenetics, NIAAA, National Institutes of Health, Bethesda, Maryland 20892

8,5'-Cyclopurine-2'-deoxynucleotides, which are strong blocks to mammalian DNA and RNA polymerases, represent a novel class of oxidative DNA lesion in that they are specifically repaired by nucleotide excision repair but not by base excision repair or direct enzymatic reversion. Previous studies using thin layer chromatography of 32P-postlabeled DNA digests have detected several bulky oxidative lesions of unknown structure, called I-compounds, in DNA from normal mammalian organs. We investigated whether any of these type II I-compounds contained 8,5'-cyclo-2'-deoxyadenosine (cA). Two previously detected type II I-compounds were found to be dinucleotides of the sequence pAp-cAp and pCp-cAp. Furthermore, a modification of the technique resulted in detection of two additional I-compounds, pTp-cAp and pGp-cAp. Each I-compound isolated from neonatal rat liver DNA matched authentic 32P-labeled cA-containing chromatographic standards under nine different chromatographic conditions. Their levels increased significantly after normal birth. The 32P-postlabeling technique used here is capable of detecting 1-5 lesions/diploid mammalian cell. Thus, it should now be possible to detect changes of cA levels resulting from low level ionizing radiation and other conditions associated with oxidative stress, and to assess cA levels in tissues from patients with the genetic disease xeroderma pigmentosum who are unable to carry out nucleotide excision repair.

* This work was supported by Grants CA32157, ES04917, and AG07550 from the National Institutes of Health and by Grant GRX 325 from Shell Research, Amsterdam.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

This paper is dedicated to the memory of Erika Randerath.

** To whom correspondence should be addressed. Tel.: 301-496-7920; Fax: 301-443-8579; E-mail: pjbrooks@dicbr.niaaa.nih.gov.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
H. Cao and Y. Wang
Quantification of oxidative single-base and intrastrand cross-link lesions in unmethylated and CpG-methylated DNA induced by Fenton-type reagents
Nucleic Acids Res., July 10, 2007; (2007) gkm497v1.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Besaratinia, T. W. Synold, H.-H. Chen, C. Chang, B. Xi, A. D. Riggs, and G. P. Pfeifer
DNA lesions induced by UV A1 and B radiation in human cells: Comparative analyses in the overall genome and in the p53 tumor suppressor gene
PNAS, July 19, 2005; 102(29): 10058 - 10063.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
G.-D. Zhou, N. Popovic, J. R. Lupton, N. D. Turner, R. S. Chapkin, and K. C. Donnelly
Tissue-Specific Attenuation of Endogenous DNA I-Compounds in Rats by Carcinogen Azoxymethane: Possible Role of Dietary Fish Oil in Colon Cancer Prevention
Cancer Epidemiol. Biomarkers Prev., May 1, 2005; 14(5): 1230 - 1235.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Jaruga, J. Theruvathu, M. Dizdaroglu, and P. J. Brooks
Complete release of (5'S)-8,5'-cyclo-2'-deoxyadenosine from dinucleotides, oligodeoxynucleotides and DNA, and direct comparison of its levels in cellular DNA with other oxidatively induced DNA lesions
Nucleic Acids Res., June 23, 2004; 32(11): e87 - e87.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
B. A. Nexo, U. Vogel, A. Olsen, T. Ketelsen, Z. Bukowy, B. L. Thomsen, H. Wallin, K. Overvad, and A. Tjonneland
A specific haplotype of single nucleotide polymorphisms on chromosome 19q13.2-3 encompassing the gene RAI is indicative of post-menopausal breast cancer before age 55
Carcinogenesis, May 1, 2003; 24(5): 899 - 904.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. R. Laposa and J. E. Cleaver
DNA repair on the brain
PNAS, November 6, 2001; 98(23): 12860 - 12862.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Kuraoka, P. Robins, C. Masutani, F. Hanaoka, D. Gasparutto, J. Cadet, R. D. Wood, and T. Lindahl
Oxygen Free Radical Damage to DNA. TRANSLESION SYNTHESIS BY HUMAN DNA POLYMERASE eta AND RESISTANCE TO EXONUCLEASE ACTION AT CYCLOPURINE DEOXYNUCLEOSIDE RESIDUES
J. Biol. Chem., December 21, 2001; 276(52): 49283 - 49288.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement