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J. Biol. Chem., Vol. 276, Issue 4, 2831-2840, January 26, 2001
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From the We have characterized NORPEG, a novel
gene from human retinal pigment epithelial cells (ARPE-19), in which
its expression is induced by all-trans-retinoic acid. Two
transcripts (~3 and ~5 kilobases in size) have been detected
for this gene, which is localized to chromosome band 5p13.2-13.3.
Placenta and testis showed the highest level of expression among
various human tissues tested. Six ankyrin repeats and a long
coiled-coil domain are present in the predicted sequence of the NORPEG
protein, which contains 980 amino acid residues. This ~110-kDa
protein was transiently expressed in COS-7 cells as a FLAG fusion
protein and immunolocalized to the cytoplasm. Confocal microscopic
analysis of the NORPEG protein in ARPE-19 cells showed threadlike
projections in the cytoplasm reminiscent of the cytoskeleton.
Consistent with this localization, the expressed NORPEG protein showed
resistance to solubilization by Triton X-100 and KCl. An ortholog of
NORPEG characterized from mouse encoded a protein
that showed 91% sequence similarity to the human NORPEG protein. The
expression of Norpeg mRNA was detected in mouse embryo
at embryonic day 9.5 by in situ hybridization, and the
expression appears to be developmentally regulated. In adult mouse, the
highest level of expression was detected in the seminiferous tubules of testis.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF155135 and AF274866.
Molecular Characterization and Developmental Expression of
NORPEG, a Novel Gene Induced by Retinoic Acid*
§,,,,
,
Biochemistry Section, Laboratory of Retinal
Cell and Molecular Biology, and the Immunology and Virology
Section, Laboratory of Immunology, National Eye Institute, National
Institutes of Health, Bethesda, Maryland 20892-2740 and the
¶ Departments of Cellular Biology and Anatomy and of
Ophthalmology, Medical College of Georgia,
Augusta, Georgia 30912-2000
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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