HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 4, 2905-2913, January 26, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/4/2905    most recent M005627200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yao, X.-D. Articles by Elias, P. Search for Related Content PubMed PubMed Citation Articles by Yao, X.-D. Articles by Elias, P.

Recombination during Early Herpes Simplex Virus Type 1 Infection Is Mediated by Cellular Proteins^*

Xiao-Dan Yao and Per Elias^§¶

From the  Department of Molecular Biology and Genetics, College of Biological Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada and the ^§ Department of Medical Biochemistry, Göteborg University, Box 440, Göteborg SE 405 30, Sweden

Homologous recombination was examined in cells infected with herpes simplex virus type I. Circular and linear DNA with directly repeated sequences was introduced as recombination substrates into cells. Recombination was measured either by origin-dependent amplification of recombination products or by recombination-dependent expression of luciferase from a disrupted gene. Homologous recombination in baby hamster kidney cells converted linear DNA to circular templates for DNA replication and luciferase expression in the complete absence of virus. The products of homologous recombination were efficiently amplified by the viral replication apparatus. The efficiency of recombination was dependent on the structure of the substrate as well as the cell type. Linear DNA with the direct repeats at internal positions failed to recombine in Balb/c 3T3 cells and induced p53-dependent apoptosis. In contrast, linear DNA with directly repeated sequences precisely at the ends recombined and replicated in 3T3 cells. Homologous recombination in baby hamster kidney cells did not depend on the position of the repeated sequences. We conclude that homologous recombination is independent of viral gene functions and that it is likely to be carried out by cellular proteins. We suggest that homologous recombination between directly repeated sequences in the linear herpes simplex virus type 1 chromosome may help to avoid p53-dependent apoptosis and to promote viral DNA replication.

This article has been cited by other articles:

				I. Muylaert and P. Elias Knockdown of DNA Ligase IV/XRCC4 by RNA Interference Inhibits Herpes Simplex Virus Type I DNA Replication J. Biol. Chem., April 13, 2007; 282(15): 10865 - 10872. [Abstract] [Full Text] [PDF]

				B. L. Strang and N. D. Stow Blocks to herpes simplex virus type 1 replication in a cell line, tsBN2, encoding a temperature-sensitive RCC1 protein J. Gen. Virol., February 1, 2007; 88(2): 376 - 383. [Abstract] [Full Text] [PDF]

				B. Macao, M. Olsson, and P. Elias Functional Properties of the Herpes Simplex Virus Type I Origin-binding Protein Are Controlled by Precise Interactions with the Activated Form of the Origin of DNA Replication J. Biol. Chem., July 9, 2004; 279(28): 29211 - 29217. [Abstract] [Full Text] [PDF]

				D. E. Wilkinson and S. K. Weller Recruitment of Cellular Recombination and Repair Proteins to Sites of Herpes Simplex Virus Type 1 DNA Replication Is Dependent on the Composition of Viral Proteins within Prereplicative Sites and Correlates with the Induction of the DNA Damage Response J. Virol., May 1, 2004; 78(9): 4783 - 4796. [Abstract] [Full Text] [PDF]

				A. V. Nimonkar and P. E. Boehmer On the mechanism of strand assimilation by the herpes simplex virus type-1 single-strand DNA-binding protein (ICP8) Nucleic Acids Res., September 15, 2003; 31(18): 5275 - 5281. [Abstract] [Full Text] [PDF]

				N. B. Reuven, A. E. Staire, R. S. Myers, and S. K. Weller The Herpes Simplex Virus Type 1 Alkaline Nuclease and Single-Stranded DNA Binding Protein Mediate Strand Exchange In Vitro J. Virol., July 1, 2003; 77(13): 7425 - 7433. [Abstract] [Full Text] [PDF]

				X. Fu, H. Wang, and X. Zhang High-Frequency Intermolecular Homologous Recombination during Herpes Simplex Virus-Mediated Plasmid DNA Replication J. Virol., May 13, 2002; 76(12): 5866 - 5874. [Abstract] [Full Text] [PDF]

				A. V. Nimonkar and P. E. Boehmer In Vitro Strand Exchange Promoted by the Herpes Simplex Virus Type-1 Single Strand DNA-binding Protein (ICP8) and DNA Helicase-Primase J. Biol. Chem., April 19, 2002; 277(17): 15182 - 15189. [Abstract] [Full Text] [PDF]