Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M104185200 on July 25, 2001

J. Biol. Chem., Vol. 276, Issue 40, 37011-37019, October 5, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/40/37011    most recent
M104185200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Terraz, C.
Right arrow Articles by Rossert, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Terraz, C.
Right arrow Articles by Rossert, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

delta EF1 Binds to a Far Upstream Sequence of the Mouse Pro-alpha 1(I) Collagen Gene and Represses Its Expression in Osteoblasts*

Catherine TerrazDagger §, Dave Toman, Madeleine DelaucheDagger , Pierre RoncoDagger , and Jerome RossertDagger ||

From the Dagger  INSERM U489 and Université Paris VI, Paris, France and  Cohesion Technologies, Palo Alto, California 94303

The transcription of type I collagen genes is tightly regulated, but few cis-acting elements have been identified that can modulate the levels of expression of these genes. Generation of transgenic mice harboring various segments of the mouse pro-alpha 1(I) collagen promoter led us to suspect that a repressor element was located between -10.5 and -17 kilobase pairs. Stable and transient transfection experiments in ROS17/2.8 osteoblastic cells confirmed the existence of such a repressor element at about -14 kilobase pairs and showed that it consisted in an almost perfect three-time repeat of a 41-base pair sequence. This element, which we named COIN-1, contains three E2-boxes, and a point mutation in at least two of them completely abolished its repressor effect. In gel shift assays, COIN-1 bound a DNA-binding protein named delta EF1/ZEB-1, and mutations that abolished the repressor effect of COIN-1 also suppressed the binding of delta EF1. We also showed that the repressor effect of COIN-1 was not mediated by chromatin compaction. Furthermore, overexpression of delta EF1 in ROS17/2.8 osteoblastic cells enhanced the inhibitory effect of COIN-1 in a dose-dependent manner and repressed the expression of the pro-alpha 1(I) collagen gene. Thus, delta EF1 appears to repress the expression of the mouse pro-alpha 1(I) collagen gene, through its binding to COIN-1.

* This work was supported by grants from the Association pour la Recherche sur le Cancer (to J. R.) and from the University of Paris (to J. R.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Recipient of a fellowship from the Ministère de l'Education, de la recherche, et de la Technologie.

|| To whom correspondence should be addressed: INSERM U489, Hôpital TENON, 4 rue de la Chine, 75020 Paris, France. Tel.: 33-1-56-01-69-93; Fax: 33-1-56-01-69-99; E-mail: jerome.rossert@tnn.ap-hop-paris.fr.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 CJASNHome page
M. Kato, L. Arce, and R. Natarajan
MicroRNAs and Their Role in Progressive Kidney Diseases
Clin. J. Am. Soc. Nephrol., July 1, 2009; 4(7): 1255 - 1266.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. S. Mehta, E. N. Vithana, D. T. H. Tan, V. H. K. Yong, G. H. F. Yam, R. W. K. Law, W. G. W. Chong, C. P. Pang, and T. Aung
Analysis of the Posterior Polymorphous Corneal Dystrophy 3 Gene, TCF8, in Late-Onset Fuchs Endothelial Corneal Dystrophy
Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 184 - 188.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Lejard, G. Brideau, F. Blais, R. Salingcarnboriboon, G. Wagner, M. H. A. Roehrl, M. Noda, D. Duprez, P. Houillier, and J. Rossert
Scleraxis and NFATc Regulate the Expression of the Pro-{alpha}1(I) Collagen Gene in Tendon Fibroblasts
J. Biol. Chem., June 15, 2007; 282(24): 17665 - 17675.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. S. Spoelstra, N. G. Manning, Y. Higashi, D. Darling, M. Singh, K. R. Shroyer, R. R. Broaddus, K. B. Horwitz, and J. K. Richer
The Transcription Factor ZEB1 Is Aberrantly Expressed in Aggressive Uterine Cancers.
Cancer Res., April 1, 2006; 66(7): 3893 - 3902.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Matsuo, S. Tanaka, M. K. Gordon, M. Koch, H. Yoshioka, and F. Ramirez
CREB-AP1 Protein Complexes Regulate Transcription of the Collagen XXIV Gene (Col24a1) in Osteoblasts
J. Biol. Chem., March 3, 2006; 281(9): 5445 - 5452.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. T. Antoniv, S. Tanaka, B. Sudan, S. De Val, K. Liu, L. Wang, D. J. Wells, G. Bou-Gharios, and F. Ramirez
Identification of a Repressor in the First Intron of the Human {alpha}2(I) Collagen Gene (COL1A2)
J. Biol. Chem., October 21, 2005; 280(42): 35417 - 35423.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Ponticos, T. Partridge, C. M. Black, D. J. Abraham, and G. Bou-Gharios
Regulation of Collagen Type I in Vascular Smooth Muscle Cells by Competition between Nkx2.5 and {delta}EF1/ZEB1
Mol. Cell. Biol., July 15, 2004; 24(14): 6151 - 6161.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. N. Maclean, E. Kraus, and J. P. Kraus
The Dominant Role of Sp1 in Regulating the Cystathionine {beta}-Synthase -1a and -1b Promoters Facilitates Potential Tissue-specific Regulation by Kruppel-like Factors
J. Biol. Chem., March 5, 2004; 279(10): 8558 - 8566.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
O. Rahkonen, M. Su, H. Hakovirta, I. Koskivirta, S. G. Hormuzdi, E. Vuorio, P. Bornstein, and R. Penttinen
Mice With a Deletion in the First Intron of the Col1a1 Gene Develop Age-Dependent Aortic Dissection and Rupture
Circ. Res., January 9, 2004; 94(1): 83 - 90.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Christmas, N. Carlesso, H. Shang, S.-M. Cheng, B. M. Weber, F. I. Preffer, D. T. Scadden, and R. J. Soberman
Myeloid Expression of Cytochrome P450 4F3 Is Determined by a Lineage-specific Alternative Promoter
J. Biol. Chem., June 27, 2003; 278(27): 25133 - 25142.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. Sooy and M. B. Demay
Transcriptional Repression of the Rat Osteocalcin Gene by {delta}EF1
Endocrinology, September 1, 2002; 143(9): 3370 - 3375.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement