HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 41, 37815-37820, October 12, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/41/37815    most recent M104735200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Derjuga, A. Articles by Th'ng, J. P. H. Search for Related Content PubMed PubMed Citation Articles by Derjuga, A. Articles by Th'ng, J. P. H. Social Bookmarking                      What's this?

Expression of p21^Waf1/Cip1 and Cyclin D1 Is Increased in Butyrate-resistant HeLa Cells^*

Anna Derjuga, Christina Richard^§, Milena Crosato, Paul S. Wright^¶, Lorraine Chalifour, Joe Valdez, Anna Barraso, Harry A. Crissman, Walter Nishioka^**, E. Morton Bradbury, and John P. H. Th'ng^§

From the  Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, Quebec, H3T 1E2, Canada, ^¶ Aventis Pharmaceutical Inc., Bridgewater, New Jersey 08807,  Los Alamos National Laboratories, New Mexico 87545, ^** Vical Inc., San Diego, California 92121, and ^§ Northwestern Ontario Regional Cancer Center, Thunder Bay, Ontario P7A 7T1, Canada

Sodium butyrate induced cell cycle arrest in mammalian cells through an increase in p21^Waf1/Cip1, although another study showed that this arrest is related to pRB signaling. We isolated variants of HeLa cells adapted to growth in 5 mM butyrate. One of these variants, clone 5.1, constitutively expressed elevated levels of p21^Waf1/Cip1 when incubated in regular growth medium and in the presence of butyrate. Despite this elevated level of p21^Waf1/Cip1, the cells continue to proliferate, albeit at a slower rate than parental HeLa cells. Western blot analyses showed that other cell cycle regulatory proteins were not up-regulated to compensate for the elevated expression of p21^Waf1/Cip1. However, cyclin D1 was down-regulated by butyrate in HeLa cells but not in clone 5.1. We conclude that continued expression of cyclin D1 allowed clone 5.1 to grow in the presence of butyrate and elevated levels of p21^Waf1/Cip1.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				C. Richard, D. Matthews, W. Duivenvoorden, J. Yau, P. S. Wright, and J. P.H. Th'ng Flavopiridol Sensitivity of Cancer Cells Isolated from Ascites and Pleural Fluids Clin. Cancer Res., May 1, 2005; 11(9): 3523 - 3529. [Abstract] [Full Text] [PDF]

				R. L. Piekarz, R. W. Robey, Z. Zhan, G. Kayastha, A. Sayah, A. H. Abdeldaim, S. Torrico, and S. E. Bates T-cell lymphoma as a model for the use of histone deacetylase inhibitors in cancer therapy: impact of depsipeptide on molecular markers, therapeutic targets, and mechanisms of resistance Blood, June 15, 2004; 103(12): 4636 - 4643. [Abstract] [Full Text] [PDF]

				C. Kennedy, K. Byth, C. L. Clarke, and A. deFazio Cell Proliferation in the Normal Mouse Mammary Gland and Inhibition by Phenylbutyrate Mol. Cancer Ther., October 1, 2002; 1(12): 1025 - 1033. [Abstract] [Full Text] [PDF]