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Originally published In Press as doi:10.1074/jbc.M107032200 on July 30, 2001

J. Biol. Chem., Vol. 276, Issue 41, 37834-37838, October 12, 2001
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The Gene ygdP, Associated with the Invasiveness of Escherichia coli K1, Designates a Nudix Hydrolase, Orf176, Active on Adenosine (5')-Pentaphospho-(5')-adenosine (Ap5A)*

Maurice J. BessmanDagger , Joseph D. Walsh, Christopher A. Dunn, Jyothishmathi Swaminathan, John E. Weldon, and Jianying Shen

From the Department of Biology and the McCollum-Pratt Institute, The Johns Hopkins University, Baltimore, Maryland 21218

ygdP, a gene associated with the invasion of brain microvascular endothelial cells by Escherichia coli K1 (Badger, J. L., Wass, C. A., and Kim, K. S. (2000) Mol. Microbiol. 36, 174-182), the primary Gram-negative bacterium causing meningitis in newborns, has been cloned and expressed in E. coli. The protein, YgdP, was purified to near homogeneity and identified as a member of the Nudix hydrolase subfamily of dinucleoside oligophosphate pyrophosphatases. It catalyzes the hydrolysis of diadenosine tetra-, penta-, and hexa-phosphates with a preference for diadenosine penta-phosphate, from which it forms ATP and ADP. The enzyme has a requirement for a divalent metal cation that can be met with Mg2+, Zn2+, or Mn2+ and, like most of the Nudix hydrolases, has an alkaline pH optimum between 8.5 and 9. This is the second identification of a gene associated with the invasiveness of a human pathogen as a member of the Nudix hydrolase subfamily of dinucleoside oligophosphate pyrophosphatases, and an examination of homologous proteins in other invasive bacteria suggests that this may be a common feature of cellular invasion.

* This work was supported by Grant GM 18649 from the National Institutes of Health and is Publication 1524 from the McCollum-Pratt Institute.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 410-516-7316; Fax: 410-516-5213; E-mail: zoot@jhu.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


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