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J. Biol. Chem., Vol. 276, Issue 41, 38320-38328, October 12, 2001
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From the Instituto de Bioquímica Vegetal y
Fotosíntesis, Universidad de Sevilla-Consejo Superior de
Investigaciones Científicas, Centro de Investigaciones
Científicas Isla de la Cartuja, Avenida Américo
Vespucio s/n, Isla de la Cartuja, E-41092 Sevilla, Spain
The regulatory circuits that control nitrogen
metabolism are relatively well known in several bacterial model groups.
However, much less is understood about how the nitrogen status of the
cell is perceived in vivo. In cyanobacteria, the
transcription factor NtcA is required for regulation (activation or
repression) of an extensive number of genes involved in nitrogen
metabolism. In contrast, how NtcA activity is regulated is largely
unknown. Assimilation of ammonium by most microorganisms occurs through the sequential action of two enzymes: glutamine synthetase (GS) and
glutamate synthase. Interestingly, regulation of the expression of NtcA-dependent genes in the cyanobacterium
Synechocystis sp. PCC 6803 is altered in mutants with
modified levels of GS activity. Two types of mutants were analyzed:
glnA null mutants that lack GS type I and gif
mutants unable to inactivate GS in the presence of ammonium. Changes in
the intracellular pools of 19 different amino acids and the keto acid
2-oxoglutarate were recorded in wild-type and mutant strains under
different nitrogen conditions. Our data strongly indicate that the
nitrogen status in cyanobacteria is perceived as changes in the
intracellular 2-oxoglutarate pool.
Cyanobacteria Perceive Nitrogen Status by Sensing Intracellular
2-Oxoglutarate Levels*
*
This work was supported by Grant PB97-0732 from Direccion
General de Enseñanza Superior e Investigacion Científica and
by Junta de Andalucía (Group CV1-0112).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 34-5-4489518;
Fax: 34-5-4620154; E-mail: floren@cica.es.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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