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Originally published In Press as doi:10.1074/jbc.M106610200 on August 16, 2001
J. Biol. Chem., Vol. 276, Issue 42, 38877-38884, October 19, 2001
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From the Department of Pharmacology, University of Medicine and
Dentistry of New Jersey-Robert Wood Johnson Medical School,
Piscataway, New Jersey 08854
The NUDF protein of Aspergillus
nidulans, which is required for nuclear migration through the
fungal mycelium, closely resembles the LIS1 protein required for
migration of neurons to the cerebral cortex in humans. Genetic
experiments suggested that NUDF influences nuclear migration by
affecting cytoplasmic dynein. NUDF interacts with another protein,
NUDE, which also affects nuclear migration in A. nidulans.
Interactions among LIS1, NUDE, dynein, and
The LIS1-related Protein NUDF of Aspergillus
nidulans and Its Interaction Partner NUDE Bind Directly to
Specific Subunits of Dynein and Dynactin and to
- and
-Tubulin*
-tubulin have been
demonstrated in animal cells. In this paper we examine the interactions
of the A. nidulans NUDF and NUDE proteins with components
of dynein, dynactin, and with 
- and -tubulin. We show that NUDF
binds directly to - and -tubulin and to the first P-loop of
the cytoplasmic dynein heavy chain, whereas NUDE binds directly to -
and -tubulin, to NUDK (actin-related protein 1), and to the NUDG
dynein LC8 light chain. The data suggest a direct role for NUDF in
regulation of the dynein heavy chain and an effect on other
dynein/dynactin subunits via NUDE. The interactions between NUDE, NUDF,
and -tubulin suggest that this protein may also be involved in the
regulation of dynein function. Additive interactions between NUDE and
dynein and dynactin subunits suggest that NUDE acts as a scaffolding
factor between components.
*
This work was supported by National Institutes of Health
Grant 5R01GM52309 and by the Deutsche Forschungsgemeinschaft.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of
Pharmacology, University of Medicine and Dentistry of New
Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway,
NJ 08854. Tel.: 732-235-4081; Fax: 732-235-4073; E-mail:
morrisnr@umdnj.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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