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J. Biol. Chem., Vol. 276, Issue 42, 38899-38910, October 19, 2001
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From the Institut für Zellbiologie,
Ludwig-Maximilians-Universität,
80336 München, Germany
The CD36/LIMPII family is ubiquitously expressed
in higher eukaryotes and consists of integral membrane proteins that
have in part been characterized as cell adhesion receptors, scavenger receptors, or fatty acid transporters. However, no physiological role
has been defined so far for the members of this family that localize
specifically to vesicular compartments rather than to the cell surface,
namely lysosomal integral membrane protein type II (LIMPII) from
mammals and LmpA from the amoeba Dictyostelium discoideum.
LmpA, the first described CD36/LIMPII homologue from lower eukaryotes,
has initially been identified as a suppressor of the profilin-minus
phenotype. We report the discovery and initial characterization of two
new CD36/LIMPII-related proteins, both of which share several features
with LmpA: (i) their size is considerably larger than that of the
CD36/LIMPII proteins from higher eukaryotes; (ii) they show the
characteristic "hairpin" topology of this protein family; (iii)
they are heavily N-glycosylated; and (iv) they localize to
vesicular structures of putative endolysosomal origin. However, they
show intriguing differences in their developmental regulation and
exhibit different sorting signals of the di-leucine or tyrosine-type in
their carboxyl-terminal tail domains. These features make them promising candidates as a paradigm for the study of the function and
evolution of the as yet poorly understood CD36/LIMPII proteins.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF238324 and AF238325.
Characterization of CD36/LIMPII Homologues in
Dictyostelium discoideum*
,
*
This work was supported by grants from the Deutsche
Forschungsgemeinschaft and the Fonds der Chemischen Industrie.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address and to whom correspondence should be addressed:
Cellular Morphogenesis and Signalisation, UMR 144 CNRS-Institut Curie,
25 Rue d'Ulm, 75248 Paris, Cedex 05, France. Tel.: 33 1 42 34 63 61;
Fax: 33 1 42 34 63 77; E-mail: klaus-peter.janssen@curie.fr.
§
Present address: Institut für Biochemie I, Med. Einrichtungen
der Universität zu Köln, Joseph-Stelzmann-Strasse 52, 50931 Köln, Germany.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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