JBC Invitrogen Ultrasensitive Cytokine Assays

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M104488200 on August 1, 2001

J. Biol. Chem., Vol. 276, Issue 43, 40313-40318, October 26, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/43/40313    most recent
M104488200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liang, X.-h.
Right arrow Articles by Michaeli, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liang, X.-h.
Right arrow Articles by Michaeli, S.

Identification of the First Trypanosome H/ACA RNA That Guides Pseudouridine Formation on rRNA*

Xue-hai Liang, Li Liu, and Shulamit MichaeliDagger

From the Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel

In trypanosomes small nucleolar RNA (snoRNA) genes are clustered, and the clusters encode for either single or multiple RNAs. We previously reported on a genomic locus in Leptomonas collosoma that encodes for multiple C/D snoRNAs whose expression is regulated at the processing level (Xu, Y., Liu, L., Lopez-Estraño, C., and Michaeli, S. (2001) J. Biol. Chem. 276, 14289-14298). In this study we have characterized, in the same genomic locus, the first trypanosome H/ACA RNA, which we termed h1. Having a length of 69 nucleotides, h1 has the potential to guide pseudouridylation on 28 S rRNA. The h1 is processed from a long polycistronic transcript that carries both the C/D and h1 snoRNAs. The h1/rRNA duplex obeys the rules for guiding pseudouridylation. Mapping of the pseudouridine site indicated that the predicted U is indeed modified. However, in contrast to all H/ACA RNAs, h1 consists of a single hairpin structure and is the shortest H/ACA RNA described so far.

* The research was supported in part by an International Research Scholars grant (to S. M.) from the Howard Hughes Medical Institute.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AY046598.

Dagger To whom correspondence should be addressed. Tel.: 972-3-5318068; Fax: 972-3-5351824; E-mail: michaes@mail.biu.ac.il.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
S. Barth, B. Shalem, A. Hury, I. D. Tkacz, X.-h. Liang, S. Uliel, I. Myslyuk, T. Doniger, M. Salmon-Divon, R. Unger, et al.
Elucidating the Role of C/D snoRNA in rRNA Processing and Modification in Trypanosoma brucei
Eukaryot. Cell, January 1, 2008; 7(1): 86 - 101.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
X. Chen, T. S. Rozhdestvensky, L. J. Collins, J. Schmitz, and D. Penny
Combined experimental and computational approach to identify non-protein-coding RNAs in the deep-branching eukaryote Giardia intestinalis
Nucleic Acids Res., July 9, 2007; 35(14): 4619 - 4628.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
X.-h. Liang, A. Hury, E. Hoze, S. Uliel, I. Myslyuk, A. Apatoff, R. Unger, and S. Michaeli
Genome-Wide Analysis of C/D and H/ACA-Like Small Nucleolar RNAs in Leishmania major Indicates Conservation among Trypanosomatids in the Repertoire and in Their rRNA Targets
Eukaryot. Cell, March 1, 2007; 6(3): 361 - 377.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
M. KHANNA, H. WU, C. JOHANSSON, M. CAIZERGUES-FERRER, and J. FEIGON
Structural study of the H/ACA snoRNP components Nop10p and the 3' hairpin of U65 snoRNA.
RNA, January 1, 2006; 12(1): 40 - 52.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
X.-H. LIANG, S. ULIEL, A. HURY, S. BARTH, T. DONIGER, R. UNGER, and S. MICHAELI
A genome-wide analysis of C/D and H/ACA-like small nucleolar RNAs in Trypanosoma brucei reveals a trypanosome-specific pattern of rRNA modification
RNA, May 1, 2005; 11(5): 619 - 645.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
A. K. HENRAS, R. CAPEYROU, Y. HENRY, and M. CAIZERGUES-FERRER
Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs
RNA, November 18, 2004; 10(11): 1704 - 1712.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
A. G. RUSSELL, M. N. SCHNARE, and M. W. GRAY
Pseudouridine-guide RNAs and other Cbf5p-associated RNAs in Euglena gracilis
RNA, July 1, 2004; 10(7): 1034 - 1046.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X.-h. Liang, A. Ochaion, Y.-x. Xu, Q. Liu, and S. Michaeli
Small Nucleolar RNA Clusters in Trypanosomatid Leptomonas collosoma: GENOME ORGANIZATION, EXPRESSION STUDIES, AND THE POTENTIAL ROLE OF SEQUENCES PRESENT UPSTREAM FROM THE FIRST REPEATED CLUSTER
J. Biol. Chem., February 13, 2004; 279(7): 5100 - 5109.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
X.-h. Liang, A. Haritan, S. Uliel, and S. Michaeli
trans and cis Splicing in Trypanosomatids: Mechanism, Factors, and Regulation
Eukaryot. Cell, October 1, 2003; 2(5): 830 - 840.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X.-h. Liang, Q. Liu, and S. Michaeli
Small nucleolar RNA interference induced by antisense or double-stranded RNA in trypanosomatids
PNAS, June 24, 2003; 100(13): 7521 - 7526.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. S. Rozhdestvensky, T. H. Tang, I. V. Tchirkova, J. Brosius, J.-P. Bachellerie, and A. Huttenhofer
Binding of L7Ae protein to the K-turn of archaeal snoRNAs: a shared RNA binding motif for C/D and H/ACA box snoRNAs in Archaea
Nucleic Acids Res., February 1, 2003; 31(3): 869 - 877.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T.-H. Tang, J.-P. Bachellerie, T. Rozhdestvensky, M.-L. Bortolin, H. Huber, M. Drungowski, T. Elge, J. Brosius, and A. Huttenhofer
Identification of 86 candidates for small non-messenger RNAs from the archaeon Archaeoglobusfulgidus
PNAS, May 28, 2002; 99(11): 7536 - 7541.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.