|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 44, 40680-40686, November 2, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Vaccinia virus gene expression is temporally
regulated, and three gene classes have been identified: early,
intermediate, and late. Several virus-encoded proteins and an activity
designated VLTF-X are required for maximum transcription in
vitro of a template containing a late promoter. VLTF-X is present
in both cytoplasmic and nuclear extracts prepared from uninfected
mammalian cells and co-purifies with a late promoter DNA-binding
activity. Here, extensive purification of VLTF-X has revealed that
heterogeneous nuclear ribonucleoproteins A2/B1 and RBM3 co-purified
with in vitro late transcription stimulation.
Overexpression and purification of these proteins from
Escherichia coli demonstrated that they both complemented
for VLTF-X activity in in vitro transcription reactions.
These studies identify two host cell factors potentially contributing
to poxvirus replication in vivo.
Vaccinia Virus Late Transcription Is Activated in
Vitro by Cellular Heterogeneous Nuclear Ribonucleoproteins*
§,, and Department of Pathology and Laboratory
Medicine, Medical University of South Carolina, and the ¶ Biology
Department, College of Charleston,
Charleston, South Carolina 29424
*
This work was supported in part by National Institutes of
Health Grant AI43329 and by the Medical University of South Carolina Institutional Research Funds of 1998-1999 and 1999-2000.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. A. Knutson, J. Oh, and S. S. Broyles Downregulation of vaccinia virus intermediate and late promoters by host transcription factor YY1 J. Gen. Virol., July 1, 2009; 90(7): 1592 - 1599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kessler, G. Sezonov, J. I. Guijarro, N. Desnoues, T. Rose, M. Delepierre, S. D. Bell, and D. Prangishvili A novel archaeal regulatory protein, Sta1, activates transcription from viral promoters Nucleic Acids Res., October 18, 2006; 34(17): 4837 - 4845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. M. Cristea, J.-W. N. Carroll, M. P. Rout, C. M. Rice, B. T. Chait, and M. R. MacDonald Tracking and Elucidating Alphavirus-Host Protein Interactions J. Biol. Chem., October 6, 2006; 281(40): 30269 - 30278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Knutson, X. Liu, J. Oh, and S. S. Broyles Vaccinia Virus Intermediate and Late Promoter Elements Are Targeted by the TATA-Binding Protein J. Virol., July 15, 2006; 80(14): 6784 - 6793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Oh and S. S. Broyles Host Cell Nuclear Proteins Are Recruited to Cytoplasmic Vaccinia Virus Replication Complexes J. Virol., October 15, 2005; 79(20): 12852 - 12860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Dresios, A. Aschrafi, G. C. Owens, P. W. Vanderklish, G. M. Edelman, and V. P. Mauro Cold stress-induced protein Rbm3 binds 60S ribosomal subunits, alters microRNA levels, and enhances global protein synthesis PNAS, February 8, 2005; 102(6): 1865 - 1870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Katsafanas and B. Moss Vaccinia Virus Intermediate Stage Transcription Is Complemented by Ras-GTPase-activating Protein SH3 Domain-binding Protein (G3BP) and Cytoplasmic Activation/Proliferation-associated Protein (p137) Individually or as a Heterodimer J. Biol. Chem., December 10, 2004; 279(50): 52210 - 52217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Broyles, M. Kremer, and B. A. Knutson Antiviral Activity of Distamycin A against Vaccinia Virus Is the Result of Inhibition of Postreplicative mRNA Synthesis J. Virol., February 15, 2004; 78(4): 2137 - 2141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Broyles Vaccinia virus transcription J. Gen. Virol., September 1, 2003; 84(9): 2293 - 2303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. V. Castro, T. M. U. Carvalho, N. Moussatche, and C. R. A. Damaso Redistribution of Cyclophilin A to Viral Factories during Vaccinia Virus Infection and Its Incorporation into Mature Particles J. Virol., August 15, 2003; 77(16): 9052 - 9068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Mohamed and E. G. Niles UUUUUNU Oligonucleotide Stimulation of Vaccinia Virus Early Gene Transcription Termination, in Trans J. Biol. Chem., March 28, 2003; 278(14): 11794 - 11801. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |