Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M105572200 on August 27, 2001

J. Biol. Chem., Vol. 276, Issue 44, 40811-40816, November 2, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/44/40811    most recent
M105572200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, H.
Right arrow Articles by Wang, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, H.
Right arrow Articles by Wang, Z.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Different Subtypes of alpha 1-Adrenoceptor Modulate Different K+ Currents via Different Signaling Pathways in Canine Ventricular Myocytes*

Huizhen WangDagger §, Baofeng Yang||, Yiqiang ZhangDagger , Hong HanDagger **, Jingxiong WangDagger , Hong ShiDagger , and Zhiguo WangDagger Dagger Dagger §§

From the Dagger  Research Center, Montreal Heart Institute, Montreal, Quebec H1T 1C8, Canada, the Dagger Dagger  Department of Medicine, University of Montreal, Montreal, Quebec H3C 3J7, Canada, and the || Department of Pharmacology, Harbin Medical University, Harbin, Heilongjiang 150086, China

Multiple subtypes (alpha 1A, alpha 1B, and alpha 1D) of alpha 1-adrenoreceptors (alpha 1ARs) co-exist in the heart and mediate a variety of cellular functions. We studied alpha 1AR modulation of inward rectifier (IK1) and transient outward (Ito) K+ currents in canine ventricular myocytes. Phenylephrine at 10 µM depressed only Ito without affecting IK1 and at 100 µM inhibited both Ito and IK1. The effect of phenylephrine on Ito was abolished by (+)niguldipine (10 nM) to inhibit alpha 1AARs but not by chloroethyclonidine (10 µM) to inactivate alpha 1BARs nor by BMY-7378 to antagonize alpha 1DARs. In contrast, phenylephrine inhibition of IK1 was reversed only by BMY-7378 (1 nM). PDD (100 nM, phorbol ester activator of protein kinase C (PKC)) simulates and bisindolylmaleimide (50 nM, PKC inhibitor) weakens phenylephrine modulation of Ito but not IK1. Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor KN-93 and inhibitor peptides abolished the effects of phenylephrine on IK1. Enhancement of PKC or CaMKII activities was seen in alpha 1aAR- or alpha 1dAR-transfected HEK293 cells and in myocytes pretreated with 10 or 100 µM phenylephrine, respectively. Our data suggest that different subtypes of alpha 1ARs selectively modulate different cardiac K+ currents via different signal transduction mechanisms; alpha 1AARs mediate Ito regulation via PKC, and alpha 1DARs mediate IK1 regulation via CaMKII.

* This work was supported in part by funds from the Canadian Institute of Health Research, the Heart and Stroke Foundation of Quebec, and the Fonds de la Recherche de l'Institut de Cardiologie de Montreal (to Z. W.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Research fellow of the Canadian Institute of Health Research.

These authors contributed equally to this work.

** Research fellow of the Heart and Stroke Foundation of Canada.

§§ Research scholar of the Heart and Stroke Foundation of Canada. To whom correspondence should be addressed: Research Center, Montreal Heart Inst., 5000 Belanger East, Montreal, PQ H1T 1C8, Canada. Tel.: 514-376-3330; Fax: 514-376-1355; E-mail: wangz@icm.umontreal.ca.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Cardiovasc ResHome page
N. Voigt, A. Friedrich, M. Bock, E. Wettwer, T. Christ, M. Knaut, R. H. Strasser, U. Ravens, and D. Dobrev
Differential phosphorylation-dependent regulation of constitutively active and muscarinic receptor-activated IK,ACh channels in patients with chronic atrial fibrillation
Cardiovasc Res, June 1, 2007; 74(3): 426 - 437.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. A.G. van der Heyden, T. J.M. Wijnhoven, and T. Opthof
Molecular aspects of adrenergic modulation of the transient outward current
Cardiovasc Res, August 1, 2006; 71(3): 430 - 442.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Yatani, D.-Z. Xu, K. Irie, K. Sano, A. Jidarian, S. F. Vatner, and E. A. Deitch
Dual effects of mesenteric lymph isolated from rats with burn injury on contractile function in rat ventricular myocytes
Am J Physiol Heart Circ Physiol, February 1, 2006; 290(2): H778 - H785.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. O-Uchi, K. Komukai, Y. Kusakari, T. Obata, K. Hongo, H. Sasaki, and S. Kurihara
{alpha}1-Adrenoceptor stimulation potentiates L-type Ca2+ current through Ca2+/calmodulin-dependent PK II (CaMKII) activation in rat ventricular myocytes
PNAS, June 28, 2005; 102(26): 9400 - 9405.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Gallego, R. Setien, L. Puebla, M. d. C. Boyano-Adanez, E. Arilla, and O. Casis
{alpha}1-Adrenoceptors stimulate a G{alpha}s protein and reduce the transient outward K+ current via a cAMP/PKA-mediated pathway in the rat heart
Am J Physiol Cell Physiol, March 1, 2005; 288(3): C577 - C585.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
S. C. M. Choisy, J. C. Hancox, L. A. Arberry, A. M. Reynolds, M. J. Shattock, and A. F. James
Evidence for a Novel K+ Channel Modulated by {alpha}1A-Adrenoceptors in Cardiac Myocytes
Mol. Pharmacol., September 1, 2004; 66(3): 735 - 748.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
S. V. P. Jones
Role of the Small GTPase Rho in Modulation of the Inwardly Rectifying Potassium Channel Kir2.1.
Mol. Pharmacol., October 1, 2003; 64(4): 987 - 993.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Zhang, H. Han, J. Wang, H. Wang, B. Yang, and Z. Wang
Impairment of Human Ether-a-Go-Go-related Gene (HERG) K+ Channel Function by Hypoglycemia and Hyperglycemia. SIMILAR PHENOTYPES BUT DIFFERENT MECHANISMS
J. Biol. Chem., March 14, 2003; 278(12): 10417 - 10426.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
H. Wang, Y. Zhang, L. Cao, H. Han, J. Wang, B. Yang, S. Nattel, and Z. Wang
HERG K+ Channel, a Regulator of Tumor Cell Apoptosis and Proliferation
Cancer Res., September 1, 2002; 62(17): 4843 - 4848.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement