|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 44, 41175-41181, November 2, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Activation of caspases results in the disruption
of structural and signaling networks in apoptotic cells. Recent
biochemical and cell biological studies have shown that components of
the cadherin-catenin adhesion complex in epithelial adherens junctions are targeted by caspases during apoptosis. In epithelial cells, desmosomes represent a second type of anchoring junctions mediating strong cell-cell contacts. Using antibodies directed against a set of
desmosomal proteins, we show that desmosomes are proteolytically targeted during apoptosis. Desmogleins and desmocollins,
representing desmosome-specific members of the cadherin superfamily of
cell adhesion molecules, are specifically cleaved after onset of
apoptosis. Similar to E-cadherin, the desmoglein-3 cytoplasmic tail is
cleaved by caspases. In addition the extracellular domains of
desmoglein-3 and desmocollin-3 are released from the cell surface by a
metalloproteinase activity. In the presence of caspase and/or
metalloproteinase inhibitors, both cleavage reactions are almost
completely inhibited. As reported previously, the desmosomal plaque
protein plakoglobin is cleaved by caspase-3 during apoptosis. Our
studies now show that plakophilin-1 and two other major plaque
proteins, desmoplakin-1 and -2, are also cleaved by caspases.
Immunofluorescence analysis confirmed that this cleavage results in the
disruption of the desmosome structure and thus contributes to cell
rounding and disintegration of the intermediate filament system.
The Fate of Desmosomal Proteins in Apoptotic
Cells*
,
,, and**
Institute of Clinical Chemistry and
Pathobiochemistry, University Hospital Benjamin Franklin,
Hindenburgdamm 30, 12200 Berlin, the § Institute of
Pharmacology, Thielallee 69-73, 14195 Berlin, the ¶ Institute of
Biochemistry, Fabeckstrasse 36A, 14195 Berlin, and the Molecular
Biology Group, Medical Faculty of the University of Halle,
06097 Halle, Germany
*
This work was supported by grants from the Volkswagen
Foundation and the Sonnenfeld Foundation and by Grant HU881/1-1 from the Deutsche Forschungsgemeinschaft.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. E. Lee, P. Berkowitz, P. S. Jolly, L. A. Diaz, M. P. Chua, and D. S. Rubenstein Biphasic Activation of p38MAPK Suggests That Apoptosis Is a Downstream Event in Pemphigus Acantholysis J. Biol. Chem., May 1, 2009; 284(18): 12524 - 12532. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Li, M. Zhao, J. Wang, Z. Liu, and L. A. Diaz Involvement of the Apoptotic Mechanism in Pemphigus Foliaceus Autoimmune Injury of the Skin J. Immunol., January 1, 2009; 182(1): 711 - 717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zahn, A. Jaschke, J. Weiske, A. Hommel, D. Hesse, R. Augustin, L. Lu, W. Hong, S. Florian, A. Scheepers, et al. ADP-ribosylation Factor-like GTPase ARFRP1 Is Required for Trans-Golgi to Plasma Membrane Trafficking of E-cadherin J. Biol. Chem., October 3, 2008; 283(40): 27179 - 27188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Weiske, K. F. Albring, and O. Huber The tumor suppressor Fhit acts as a repressor of -catenin transcriptional activity PNAS, December 18, 2007; 104(51): 20344 - 20349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Nava, M. G. Laukoetter, A. M. Hopkins, O. Laur, K. Gerner-Smidt, K. J. Green, C. A. Parkos, and A. Nusrat Desmoglein-2: A Novel Regulator of Apoptosis in the Intestinal Epithelium Mol. Biol. Cell, November 1, 2007; 18(11): 4565 - 4578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. W. Okunade, M. L. Miller, M. Azhar, A. Andringa, L. P. Sanford, T. Doetschman, V. Prasad, and G. E. Shull Loss of the Atp2c1 Secretory Pathway Ca2+-ATPase (SPCA1) in Mice Causes Golgi Stress, Apoptosis, and Midgestational Death in Homozygous Embryos and Squamous Cell Tumors in Adult Heterozygotes J. Biol. Chem., September 7, 2007; 282(36): 26517 - 26527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Werner, F. Chen, J. V. Moyano, F. Yehiely, J. C. R. Jones, and V. L. Cryns Caspase Proteolysis of the Integrin beta4 Subunit Disrupts Hemidesmosome Assembly, Promotes Apoptosis, and Inhibits Cell Migration J. Biol. Chem., February 23, 2007; 282(8): 5560 - 5569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bijl and C. G. Kallenberg Ultraviolet light and cutaneous lupus Lupus, November 1, 2006; 15(11): 724 - 727. [Abstract] [PDF]
|
|
|
|
|
|
J. Weiske and O. Huber The Histidine Triad Protein Hint1 Triggers Apoptosis Independent of Its Enzymatic Activity J. Biol. Chem., September 15, 2006; 281(37): 27356 - 27366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mahmoodzadeh, S. Eder, J. Nordmeyer, E. Ehler, O. Huber, P. Martus, J. Weiske, R. Pregla, R. Hetzer, and V. Regitz-Zagrosek Estrogen receptor alpha up-regulation and redistribution in human heart failure FASEB J, May 1, 2006; 20(7): 926 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Dusek, S. Getsios, F. Chen, J. K. Park, E. V. Amargo, V. L. Cryns, and K. J. Green The Differentiation-dependent Desmosomal Cadherin Desmoglein 1 Is a Novel Caspase-3 Target That Regulates Apoptosis in Keratinocytes J. Biol. Chem., February 10, 2006; 281(6): 3614 - 3624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Arredondo, A. I. Chernyavsky, A. Karaouni, and S. A. Grando Novel Mechanisms of Target Cell Death and Survival and of Therapeutic Action of IVIg in Pemphigus Am. J. Pathol., December 1, 2005; 167(6): 1531 - 1544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Weiske and O. Huber The histidine triad protein Hint1 interacts with Pontin and Reptin and inhibits TCF-{beta}-catenin-mediated transcription J. Cell Sci., July 15, 2005; 118(14): 3117 - 3129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Lorch, J. Klessner, J. K. Park, S. Getsios, Y. L. Wu, M. S. Stack, and K. J. Green Epidermal Growth Factor Receptor Inhibition Promotes Desmosome Assembly and Strengthens Intercellular Adhesion in Squamous Cell Carcinoma Cells J. Biol. Chem., August 27, 2004; 279(35): 37191 - 37200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bojarski, J. Weiske, T. Schoneberg, W. Schroder, J. Mankertz, J.-D. Schulzke, P. Florian, M. Fromm, R. Tauber, and O. Huber The specific fates of tight junction proteins in apoptotic epithelial cells J. Cell Sci., April 15, 2004; 117(10): 2097 - 2107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Amasheh, N. Meiri, A. H. Gitter, T. Schoneberg, J. Mankertz, J. D. Schulzke, and M. Fromm Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells J. Cell Sci., March 14, 2003; 115(24): 4969 - 4976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. H. Van Aken, O. De Wever, L. Van Hoorde, E. Bruyneel, J.-J. De Laey, and M. M. Mareel Invasion of Retinal Pigment Epithelial Cells: N-cadherin, Hepatocyte Growth Factor, and Focal Adhesion Kinase Invest. Ophthalmol. Vis. Sci., February 1, 2003; 44(2): 463 - 472. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |