JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M106838200 on August 24, 2001

J. Biol. Chem., Vol. 276, Issue 44, 41213-41223, November 2, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/44/41213    most recent
M106838200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, J.-Z.
Right arrow Articles by Awasthi, Y. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, J.-Z.
Right arrow Articles by Awasthi, Y. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Accelerated Metabolism and Exclusion of 4-Hydroxynonenal through Induction of RLIP76 and hGST5.8 Is an Early Adaptive Response of Cells to Heat and Oxidative Stress*

Ji-Zhong ChengDagger , Rajendra SharmaDagger , Yusong YangDagger , Sharad S. Singhal§, Abha SharmaDagger , Manjit K. SainiDagger , Shivendra V. Singh, Piotr Zimniak||, Sanjay Awasthi§, and Yogesh C. AwasthiDagger **

From the Dagger  Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555-1067, § Department of Chemistry and Biochemistry, University of Texas, Arlington, Texas 76019-0065,  Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, and || Department of Internal Medicine and Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences and Central Arkansas Veterans Healthcare System, Little Rock, Arkansas 72205

To explore the role of lipid peroxidation (LPO) products in the initial phase of stress mediated signaling, we studied the effect of mild, transient oxidative or heat stress on parameters that regulate the cellular concentration of 4-hydroxynonenal (4-HNE). When K562 cells were exposed to mild heat shock (42 °C, 30 min) or oxidative stress (50 µM H2O2, 20 min) and allowed to recover for 2 h, there was a severalfold induction of hGST5.8, which catalyzes the formation of glutathione-4-HNE conjugate (GS-HNE), and RLIP76, which mediates the transport of GS-HNE from cells (Awasthi, S., Cheng, J., Singhal, S. S., Saini, M. K., Pandya, U., Pikula, S., Bandorowicz-Pikula, J., Singh, S. V., Zimniak, P., and Awasthi, Y. C. (2000) Biochemistry 39, 9327-9334). Enhanced LPO was observed in stressed cells, but the major antioxidant enzymes and HSP70 remained unaffected. The stressed cells showed higher GS-HNE-conjugating activity and increased efflux of GS-HNE. Stress-pre-conditioned cells with induced hGST5.8 and RLIP76 acquired resistance to 4-HNE and H2O2-mediated apoptosis by suppressing a sustained activation of c-Jun N-terminal kinase and caspase 3. The protective effect of stress pre-conditioning against apoptosis was abrogated by coating the cells with anti-RLIP76 IgG, which inhibited the efflux of GS-HNE from cells, indicating that the cells acquired resistance to apoptosis by metabolizing and excluding 4-HNE at a higher rate. Induction of hGST5.8 and RLIP76 by mild, transient stress and the resulting resistance of stress-pre-conditioned cells to apoptosis appears to be a general phenomenon since it was not limited to K562 cells but was also evident in lung cancer cells, H-69, H-226, human leukemia cells, HL-60, and human retinal pigmented epithelial cells. These results strongly suggest a role of LPO products, particularly 4-HNE, in the initial phase of stress mediated signaling.

* This work was supported in part by National Institutes of Health Grants EY 04396, CA 27967 (to Y. C. A.), CA 77495 (to S. A.), CA 76348 (to S. V. S.), and ES 07804 (to P. Z.) and by a Veterans Affairs Merit Review grant (to P. Z.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

** To whom correspondence should be addressed: Dept. of Human Biological Biochemistry and Genetics, 7.138 Medical Research Bldg., UTMB, Galveston, TX 77555-1067. Tel.: 409-772-2735; Fax: 409-772-6603; E-mail: ycawasth@utmb.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. S. Singhal, S. Yadav, K. Drake, J. Singhal, and S. Awasthi
Hsf-1 and POB1 Induce Drug Sensitivity and Apoptosis by Inhibiting Ralbp1
J. Biol. Chem., July 11, 2008; 283(28): 19714 - 19729.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. Margutti, P. Matarrese, F. Conti, T. Colasanti, F. Delunardo, A. Capozzi, T. Garofalo, E. Profumo, R. Rigano, A. Siracusano, et al.
Autoantibodies to the C-terminal subunit of RLIP76 induce oxidative stress and endothelial cell apoptosis in immune-mediated vascular diseases and atherosclerosis
Blood, May 1, 2008; 111(9): 4559 - 4570.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
C. M. Ethen, C. Reilly, X. Feng, T. W. Olsen, and D. A. Ferrington
Age-Related Macular Degeneration and Retinal Protein Modification by 4-Hydroxy-2-nonenal
Invest. Ophthalmol. Vis. Sci., August 1, 2007; 48(8): 3469 - 3479.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
P. MARGUTTI, F. DELUNARDO, T. COLASANTI, E. PIRO, E. PROFUMO, R. RIGANỎ, A. SIRACUSANO, B. BUTTARI, F. CONTI, C. ALESSANDRI, et al.
Screening of Endothelial Expression Libraries for the Identification of Novel Autoantigens Involved in Distinct Autoimmune Diseases Characterized by Endothelial Dysfunction
Ann. N.Y. Acad. Sci., August 1, 2007; 1109(1): 178 - 184.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
T. Yoshida and R. M. Tuder
Pathobiology of Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease
Physiol Rev, July 1, 2007; 87(3): 1047 - 1082.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. S. Singhal, J. Singhal, S. Yadav, S. Dwivedi, P. J. Boor, Y. C. Awasthi, and S. Awasthi
Regression of Lung and Colon Cancer Xenografts by Depleting or Inhibiting RLIP76 (Ral-Binding Protein 1)
Cancer Res., May 1, 2007; 67(9): 4382 - 4389.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. V. Ramana, A. Bhatnagar, S. Srivastava, U. C. Yadav, S. Awasthi, Y. C. Awasthi, and S. K. Srivastava
Mitogenic Responses of Vascular Smooth Muscle Cells to Lipid Peroxidation-derived Aldehyde 4-Hydroxy-trans-2-nonenal (HNE): ROLE OF ALDOSE REDUCTASE-CATALYZED REDUCTION OF THE HNE-GLUTATHIONE CONJUGATES IN REGULATING CELL GROWTH
J. Biol. Chem., June 30, 2006; 281(26): 17652 - 17660.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. S. Singhal, Y. C. Awasthi, and S. Awasthi
Regression of Melanoma in a Murine Model by RLIP76 Depletion
Cancer Res., February 15, 2006; 66(4): 2354 - 2360.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
Q. Shi, J. F Vandeberg, C. Jett, K. Rice, M. M Leland, L. Talley, R. S Kushwaha, D. L Rainwater, J. L Vandeberg, and X. L. Wang
Arterial endothelial dysfunction in baboons fed a high-cholesterol, high-fat diet
Am. J. Clinical Nutrition, October 1, 2005; 82(4): 751 - 759.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Awasthi, S. S. Singhal, S. Yadav, J. Singhal, K. Drake, A. Nadkar, E. Zajac, D. Wickramarachchi, N. Rowe, A. Yacoub, et al.
RLIP76 Is a Major Determinant of Radiation Sensitivity
Cancer Res., July 15, 2005; 65(14): 6022 - 6028.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. P. Singh, T. Chen, L. Chen, N. Mei, E. McLain, V. Samokyszyn, J. J. Thaden, M. M. Moore, and P. Zimniak
Mutagenic Effects of 4-Hydroxynonenal Triacetate, a Chemically Protected Form of the Lipid Peroxidation Product 4-Hydroxynonenal, as Assayed in L5178Y/Tk+/- Mouse Lymphoma Cells
J. Pharmacol. Exp. Ther., May 1, 2005; 313(2): 855 - 861.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. Stuckler, J. Singhal, S. S. Singhal, S. Yadav, Y. C. Awasthi, and S. Awasthi
RLIP76 Transports Vinorelbine and Mediates Drug Resistance in Non-Small Cell Lung Cancer
Cancer Res., February 1, 2005; 65(3): 991 - 998.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Yang, A. Sharma, R. Sharma, B. Patrick, S. S. Singhal, P. Zimniak, S. Awasthi, and Y. C. Awasthi
Cells Preconditioned with Mild, Transient UVA Irradiation Acquire Resistance to Oxidative Stress and UVA-induced Apoptosis: ROLE OF 4-HYDROXYNONENAL IN UVA-MEDIATED SIGNALING FOR APOPTOSIS
J. Biol. Chem., October 17, 2003; 278(42): 41380 - 41388.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
R. Sharma, Y. Yang, A. Sharma, S. Dwivedi, V. L. Popov, P. J. Boor, S. S. Singhal, S. Awasthi, and Y. C. Awasthi
Mechanisms and Physiological Significance of the Transport of the Glutathione Conjugate of 4-Hydroxynonenal in Human Lens Epithelial Cells
Invest. Ophthalmol. Vis. Sci., August 1, 2003; 44(8): 3438 - 3449.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
S. Awasthi, R. Sharma, S. S. Singhal, P. Zimniak, and Y. C. Awasthi
RLIP76, a Novel Transporter Catalyzing ATP-Dependent Efflux of Xenobiotics
Drug Metab. Dispos., December 1, 2002; 30(12): 1300 - 1310.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
D. A. DICKINSON and H. J. FORMAN
Glutathione in Defense and Signaling: Lessons from a Small Thiol
Ann. N.Y. Acad. Sci., November 1, 2002; 973(1): 488 - 504.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Y. Yang, R. Sharma, J.-Z. Cheng, M. K. Saini, N. H. Ansari, U. P. Andley, S. Awasthi, and Y. C. Awasthi
Protection of HLE B-3 Cells against Hydrogen Peroxide- and Naphthalene-Induced Lipid Peroxidation and Apoptosis by Transfection with hGSTA1 and hGSTA2
Invest. Ophthalmol. Vis. Sci., February 1, 2002; 43(2): 434 - 445.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. P. Singh, A. J. Janecki, S. K. Srivastava, S. Awasthi, Y. C. Awasthi, S. J. Xia, and P. Zimniak
Membrane Association of Glutathione S-Transferase mGSTA4-4, an Enzyme That Metabolizes Lipid Peroxidation Products
J. Biol. Chem., February 1, 2002; 277(6): 4232 - 4239.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.