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Originally published In Press as doi:10.1074/jbc.M102007200 on August 23, 2001

J. Biol. Chem., Vol. 276, Issue 44, 41473-41478, November 2, 2001
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Deregulation of Electron Flow within Photosystem II in the Absence of the PsbJ Protein*

Ralf E. RegelDagger §, Natalia B. Ivleva§||**, Hagit ZerDagger Dagger , Jörg MeurerDagger , Sergey V. Shestakov||, Reinhold G. HerrmannDagger , Himadri B. Pakrasi, and Itzhak OhadDagger Dagger §§

From the Dagger  Botanisches Institute, Ludwig-Maximilians-University, Menzinger Str. 67, D-80638 München, Germany, the  Department of Biology, Washington University, St. Louis, Missouri 63130, the || Department of Genetics, Moscow State University, Moscow 119899, Russia, and the Dagger Dagger  Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

The photosystem II (PSII) complex of photosynthetic oxygen evolving membranes comprises a number of small proteins whose functions remain unknown. Here we report that the low molecular weight protein encoded by the psbJ gene is an intrinsic component of the PSII complex. Fluorescence kinetics, oxygen flash yield, and thermoluminescence measurements indicate that inactivation of the psbJ gene in Synechocystis 6803 cells and tobacco chloroplasts lowers PSII-mediated oxygen evolution activity and increases the lifetime of the reduced primary acceptor Q<UP><SUB><RM><IT>A</IT></RM></SUB><SUP><RM><IT>−</IT></RM></SUP></UP> (more than a 100-fold in the tobacco Delta psbJ mutant). The decay of the oxidized S2,3 states of the oxygen-evolving complex is considerably accelerated, and the oscillations of the Q<UP><SUB><RM><IT>B</IT></RM></SUB><SUP><RM><IT>−</IT></RM></SUP></UP>/S2,3 recombination with the number of exciting flashes are damped. Thus, PSII can be assembled in the absence of PsbJ. However, the forward electron flow from Q<UP><SUB><RM><IT>A</IT></RM></SUB><SUP><RM><IT>−</IT></RM></SUP></UP> to plastoquinone and back electron flow to the oxidized Mn cluster of the donor side are deregulated in the absence of PsbJ, thereby affecting the efficiency of PSII electron flow following the charge separation process.

* This work was supported in part by Deutsche Forschungsgemeinschaft Grants SFB 184 (to R. G. H. and I.O.) and the Fonds der Chemischen Industrie (to R. G. H.), the National Institutes of Health Grant GM 45797 and the U. S. Department of Energy (to H. B. P.), the German-Israeli foundation (GIF), and the Israeli Science Foundation administered by the Israel Academy of Sciences (to I. O.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Both authors contributed equally to this work.

** Supported in part by a training grant from the Monsanto Co. to the Plant Biology Program at Washington Univ.

§§ To whom correspondence should be addressed. Tel.: 972-2-658-5423; Fax: 972-2-658-6448; Email: ohad@vms.huji.ac.il.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.