HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 45, 41742-41747, November 9, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/45/41742    most recent M104034200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Azpiazu, I. Articles by Gautam, N. Search for Related Content PubMed PubMed Citation Articles by Azpiazu, I. Articles by Gautam, N. Social Bookmarking                      What's this?

G Protein  Subunit Interaction with a Receptor Regulates Receptor-stimulated Nucleotide Exchange^*

Inaki Azpiazu and N. Gautam^§¶

From the Departments of  Anesthesiology and ^§ Genetics, Washington University School of Medicine, St. Louis, Missouri 63110

The surfaces of heterotrimeric G proteins () in contact with receptors and the molecular events at these sites, which lead to G protein activation, are largely unknown. We show here that a peptide from the C terminus of a G protein  subunit blocks muscarinic receptor-stimulated G protein activation in a sequence-dependent fashion. A G protein mutated at the same site on the  subunit shows enhanced receptor stimulated nucleotide exchange without affecting G protein heterotrimerization. Ineffective contact between the  subunit and receptor increases the rate of receptor-stimulated nucleotide exchange. Specific interaction of the G protein  subunit with the receptor thus helps the complex to act at a distance and control guanine nucleotide exchange in the  subunit.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				D. K. Saini, V. Kalyanaraman, M. Chisari, and N. Gautam A Family of G Protein beta{gamma} Subunits Translocate Reversibly from the Plasma Membrane to Endomembranes on Receptor Activation J. Biol. Chem., August 17, 2007; 282(33): 24099 - 24108. [Abstract] [Full Text] [PDF]

				M. Akgoz, V. Kalyanaraman, and N. Gautam Receptor-mediated Reversible Translocation of the G Protein {beta}{gamma} Complex from the Plasma Membrane to the Golgi Complex J. Biol. Chem., December 3, 2004; 279(49): 51541 - 51544. [Abstract] [Full Text] [PDF]

				Q. Zhang, A. Dickson, and C. A. Doupnik G{beta}{gamma}-activated Inwardly Rectifying K+ (GIRK) Channel Activation Kinetics via G{alpha}i and G{alpha}o-coupled Receptors Are Determined by G{alpha}-specific Interdomain Interactions That Affect GDP Release Rates J. Biol. Chem., July 9, 2004; 279(28): 29787 - 29796. [Abstract] [Full Text] [PDF]

				I. Azpiazu and N. Gautam A Fluorescence Resonance Energy Transfer-based Sensor Indicates that Receptor Access to a G Protein Is Unrestricted in a Living Mammalian Cell J. Biol. Chem., June 25, 2004; 279(26): 27709 - 27718. [Abstract] [Full Text] [PDF]

				S. L. Chinault and K. J. Blumer The C-terminal Tail Preceding the CAAX Box of a Yeast G Protein {gamma} Subunit Is Dispensable for Receptor-mediated G Protein Activation in Vivo J. Biol. Chem., May 30, 2003; 278(23): 20638 - 20644. [Abstract] [Full Text] [PDF]

				S. Liu, J. J. Carrillo, J. D. Pediani, and G. Milligan Effective Information Transfer from the alpha 1b-Adrenoceptor to Galpha 11 Requires Both beta /gamma Interactions and an Aromatic Group Four Amino Acids from the C Terminus of the G Protein J. Biol. Chem., July 5, 2002; 277(28): 25707 - 25714. [Abstract] [Full Text] [PDF]

				M. Akgoz, I. Azpiazu, V. Kalyanaraman, and N. Gautam Role of the G Protein gamma Subunit in beta gamma Complex Modulation of Phospholipase Cbeta Function J. Biol. Chem., May 24, 2002; 277(22): 19573 - 19578. [Abstract] [Full Text] [PDF]