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J. Biol. Chem., Vol. 276, Issue 45, 41790-41796, November 9, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/45/41790    most recent M107401200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Carr, A. Articles by Kreikemeyer, B. Search for Related Content PubMed PubMed Citation Articles by Carr, A. Articles by Kreikemeyer, B. Social Bookmarking                      What's this?

Similarities between Complement-mediated and Streptolysin S-mediated Hemolysis^*

Abbey Carr, Darren D. Sledjeski, Andreas Podbielski^§, Michael D. P. Boyle^¶, and Bernd Kreikemeyer^§

From the  Department of Microbiology & Immunology, Medical College of Ohio, Toledo, Ohio 43614-5806 and the ^§ Department of Medical Microbiology & Hygiene, University Hospital Rostock, Schillingallee 70, D-18055 Rostock, Germany

The oxygen-stable hemolysin streptolysin S (SLS) of Streptococcus pyogenes is encoded in part by the pel/sagA gene product. Antibodies to a synthetic peptide from the C terminus of the Pel/SagA open reading frame inhibited hemolysis mediated by both culture supernatants from multiple M serotypes of S. pyogenes isolates or a commercially available SLS preparation. Analysis of the SLS-mediated hemolytic reaction demonstrated that it was temperature- and concentration-dependent. Like complement-mediated hemolysis it conforms to the prediction of a one-hit mechanism of hemolysis. A number of intermediates in the SLS-mediated hemolysis of sheep erythrocytes could be distinguished. SLS could bind to erythrocytes below 17 °C; however, lysis could only occur at temperatures >23 °C. Following binding of SLS and washing, a papain-sensitive intermediate could be distinguished prior to insertion of the SLS complex into the erythrocyte membrane, which resulted in formation of a transmembrane pore and led to irreversible osmotic lysis of the cell. These intermediates were similar to those described previously during complement-mediated hemolysis.

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