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Originally published In Press as doi:10.1074/jbc.M106660200 on September 4, 2001

J. Biol. Chem., Vol. 276, Issue 45, 42477-42484, November 9, 2001
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Structures of Yeast ARF2 and ARL1
DISTINCT ROLES FOR THE N TERMINUS IN THE STRUCTURE AND FUNCTION OF ARF FAMILY GTPases*

J. Carlos Amor, John R. Horton, Xinjun Zhu, Yi WangDagger , Cameron Sullards, Dagmar RingeDagger , Xiaodong Cheng, and Richard A. Kahn§

From the Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322-3050 and the Dagger  Department of Biochemistry and Chemistry, Rosenstiel Basic Medical Research Center, Brandeis University, Waltham, Massachusetts 02454

Structures were determined by x-ray crystallography for two members of the ADP-ribosylation factor (ARF) family of regulatory GTPases, yeast ARF1 and ARL1, and were compared with previously determined structures of human ARF1 and ARF6. These analyses revealed an overall conserved fold but differences in primary sequence and length, particularly in an N-terminal loop, lead to differences in nucleotide and divalent metal binding. Packing of hydrophobic residues is central to the interplay between the N-terminal alpha -helix, switch I, and the interswitch region, which along with differences in surface electrostatics provide explanations for the different biophysical and biochemical properties of ARF and ARF-like proteins.

* This work was supported by National Institutes of Health Grant AI43996 (to J. C. A., X. Z., J. H., and R. A. K.) and the Georgia Research Alliance.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom all correspondence should be addressed: Dept. of Biochemistry, Emory University School of Medicine, 1510 Clifton Rd., Atlanta, GA 30322-3050. Tel.: 404-727-3561; Fax: 404-727-3746; E-mail: rkahn@emory.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.