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Originally published In Press as doi:10.1074/jbc.M106917200 on September 10, 2001

J. Biol. Chem., Vol. 276, Issue 46, 42857-42862, November 16, 2001
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Fidelity and Damage Bypass Ability of Schizosaccharomyces pombe Eso1 Protein, Comprised of DNA Polymerase eta  and Sister Chromatid Cohesion Protein Ctf7*

Amy C. Madril, Robert E. Johnson, M. Todd Washington, Louise Prakash, and Satya PrakashDagger

From the Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1061

DNA polymerase eta  (Poleta ) functions in error-free bypass of ultraviolet light-induced DNA lesions, and mutational inactivation of Poleta in humans causes the cancer prone syndrome, the variant form of xeroderma pigmentosum (XPV). Both Saccharomyces cerevisiae and human Poleta efficiently insert two adenines opposite the two thymines of a cyclobutane pyrimidine dimer. Interestingly, in the fission yeast Schizosaccharomyces pombe, the eso1+ encoded protein is comprised of two domains, wherein the NH2 terminus is highly homologous to Poleta , and the COOH terminus is highly homologous to the S. cerevisiae Ctf7 protein which is essential for the establishment of sister chromatid cohesion during S phase. Here we characterize the DNA polymerase activity of S. pombe GST-Eso1 fusion protein and a truncated version containing only the Poleta domain. Both proteins exhibit a similar DNA polymerase activity with a low processivity, and steady-state kinetic analyses show that on undamaged DNA, both proteins misincorporate nucleotides with frequencies of ~10-2 to 10-3. We also examine the two proteins for their ability to replicate a cyclobutane pyrimidine dimer-containing DNA template and find that both proteins replicate through the lesion equally well. Thus, fusion with Ctf7 has no significant effect on the DNA replication or damage bypass properties of Poleta . The possible role of Ctf7 fusion with Poleta in the replication of Cohesin-bound DNA sequences is discussed.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Blocker Medical Research Bldg., 11th and Mechanic Sts., Galveston, TX 77555-1061. Tel.: 409-747-8602; Fax: 409-747-8608; E-mail: sprakash@scms.utmb.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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