JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M106244200 on September 10, 2001

J. Biol. Chem., Vol. 276, Issue 46, 43400-43406, November 16, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/46/43400    most recent
M106244200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hayashi, M.
Right arrow Articles by Moriyama, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hayashi, M.
Right arrow Articles by Moriyama, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Differentiation-associated Na+-dependent Inorganic Phosphate Cotransporter (DNPI) Is a Vesicular Glutamate Transporter in Endocrine Glutamatergic Systems*

Mitsuko HayashiDagger §, Masato OtsukaDagger , Riyo Morimoto, Sumiko Hirota, Shouki Yatsushiro, Jun Takeda, Akitsugu Yamamoto||, and Yoshinori Moriyama**

From the Department of Biochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Okayama 700-8530, the  Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, and the || Department of Physiology, Kansai Medical University, Moriguchi, Osaka 570-8506, Japan

Vesicular glutamate transporter is present in neuronal synaptic vesicles and endocrine synaptic-like microvesicles and is responsible for vesicular storage of L-glutamate. A brain-specific Na+-dependent inorganic phosphate transporter (BNPI) functions as a vesicular glutamate transporter in synaptic vesicles, and the expression of this BNPI defines the glutamatergic phenotype in the central nervous system (Bellocchio, E. E., Reimer, R. J., Fremeau, R. T., Jr., and Edwards, R. H. (2000) Science 289, 957-960; Takamori, S., Rhee, J. S., Rosenmund, C., and Jahn, R. (2000) Nature 407, 189-194). However, since not all glutamatergic neurons contain BNPI, an additional transporter(s) responsible for vesicular glutamate uptake has been postulated. Here we report that differentiation-associated Na+-dependent inorganic phosphate cotransporter (DNPI), an isoform of BNPI (Aihara, Y., Mashima, H., Onda, H., Hisano, S., Kasuya, H., Hori, T., Yamada, S., Tomura, H., Yamada, Y., Inoue, I., Kojima, I., and Takeda, J. (2000) J. Neurochem. 74, 2622-2625), also transports L-glutamate at the expense of an electrochemical gradient of protons established by the vacuolar proton pump when expressed in COS7 cells. Molecular, biological, and immunohistochemical studies have indicated that besides its presence in neuronal cells DNPI is preferentially expressed in mammalian pinealocytes, alpha TC6 cells, clonal pancreatic alpha  cells, and alpha  cells of Langerhans islets, these cells being proven to secrete L-glutamate through Ca2+-dependent regulated exocytosis followed by its vesicular storage. Pancreatic polypeptide-secreting F cells of Langerhans islets also expressed DNPI. These results constitute evidence that DNPI functions as another vesicular transporter in glutamatergic endocrine cells as well as in neurons.

* This study was supported in part by grants-in-aid for scientific research from the Ministry of Education, Science, Sports and Culture of Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation; the Salt Science Foundation; and the Mishima Kaiun Memorial Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Both authors contributed equally to this work.

§ Supported by a research fellowship from the Japan Society for Promotion of Science for Young Scientists.

** To whom correspondence should be addressed. Tel. and Fax: 81-86-251-7933; E-mail: moriyama@pheasant.pharm.okayama-u.ac.jp.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. Juge, Y. Yoshida, S. Yatsushiro, H. Omote, and Y. Moriyama
Vesicular Glutamate Transporter Contains Two Independent Transport Machineries
J. Biol. Chem., December 22, 2006; 281(51): 39499 - 39506.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
E. Hrabovszky, I. Kallo, G. F. Turi, K. May, G. Wittmann, C. Fekete, and Z. Liposits
Expression of Vesicular Glutamate Transporter-2 in Gonadotrope and Thyrotrope Cells of the Rat Pituitary. Regulation by Estrogen and Thyroid Hormone Status
Endocrinology, August 1, 2006; 147(8): 3818 - 3825.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
H. Jin, S.-K. Hwang, K. Yu, H. K. Anderson, Y.-S. Lee, K. H. Lee, A.-C. Prats, D. Morello, G. R. Beck Jr., and M.-H. Cho
A High Inorganic Phosphate Diet Perturbs Brain Growth, Alters Akt-ERK Signaling, and Results in Changes in Cap-Dependent Translation
Toxicol. Sci., March 1, 2006; 90(1): 221 - 229.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Winter, I. Brunk, D. J. Walther, M. Holtje, M. Jiang, J.-U. Peter, S. Takamori, R. Jahn, L. Birnbaumer, and G. Ahnert-Hilger
G{alpha}o2 Regulates Vesicular Glutamate Transporter Activity by Changing Its Chloride Dependence
J. Neurosci., May 4, 2005; 25(18): 4672 - 4680.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
E. Hrabovszky, G. Wittmann, G. F. Turi, Z. Liposits, and C. Fekete
Hypophysiotropic Thyrotropin-Releasing Hormone and Corticotropin-Releasing Hormone Neurons of the Rat Contain Vesicular Glutamate Transporter-2
Endocrinology, January 1, 2005; 146(1): 341 - 347.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
S. Uehara, A. Muroyama, N. Echigo, R. Morimoto, M. Otsuka, S. Yatsushiro, and Y. Moriyama
Metabotropic Glutamate Receptor Type 4 Is Involved in Autoinhibitory Cascade for Glucagon Secretion by {alpha}-Cells of Islet of Langerhans
Diabetes, April 1, 2004; 53(4): 998 - 1006.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
Y. Moriyama and A. Yamamoto
Glutamatergic Chemical Transmission: Look! Here, There, and Anywhere
J. Biochem., February 1, 2004; 135(2): 155 - 163.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. Hayashi, R. Morimoto, A. Yamamoto, and Y. Moriyama
Expression and Localization of Vesicular Glutamate Transporters in Pancreatic Islets, Upper Gastrointestinal Tract, and Testis
J. Histochem. Cytochem., October 1, 2003; 51(10): 1375 - 1390.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. Hayashi, M. Otsuka, R. Morimoto, A. Muroyama, S. Uehara, A. Yamamoto, and Y. Moriyama
Vesicular Inhibitory Amino Acid Transporter Is Present in Glucagon-Containing Secretory Granules in {alpha}TC6 Cells, Mouse Clonal {alpha}-Cells, and {alpha}-Cells of Islets of Langerhans
Diabetes, August 1, 2003; 52(8): 2066 - 2074.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Bai, X. Zhang, and F. K. Ghishan
Characterization of vesicular glutamate transporter in pancreatic alpha - and beta -cells and its regulation by glucose
Am J Physiol Gastrointest Liver Physiol, May 1, 2003; 284(5): G808 - G814.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Hayashi, H. Yamada, S. Uehara, R. Morimoto, A. Muroyama, S. Yatsushiro, J. Takeda, A. Yamamoto, and Y. Moriyama
Secretory Granule-mediated Co-secretion of L-Glutamate and Glucagon Triggers Glutamatergic Signal Transmission in Islets of Langerhans
J. Biol. Chem., January 10, 2003; 278(3): 1966 - 1974.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Gras, E. Herzog, G. C. Bellenchi, V. Bernard, P. Ravassard, M. Pohl, B. Gasnier, B. Giros, and S. El Mestikawy
A Third Vesicular Glutamate Transporter Expressed by Cholinergic and Serotoninergic Neurons
J. Neurosci., July 1, 2002; 22(13): 5442 - 5451.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
Q. Tong, R. Ouedraogo, and A. L. Kirchgessner
Localization and function of group III metabotropic glutamate receptors in rat pancreatic islets
Am J Physiol Endocrinol Metab, June 1, 2002; 282(6): E1324 - E1333.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.