|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 47, 43775-43783, November 23, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Departamento de Genética Molecular, Instituto de
Fisiología Celular, Universidad Nacional Autónoma de
México, Apartado Postal 70-242, México D.F. 04510, México
In the yeast Saccharomyces
cerevisiae, two NADP+-dependent glutamate
dehydrogenases (NADP-GDHs) encoded by GDH1 and
GDH3 catalyze the synthesis of glutamate from
ammonium and
NADP-Glutamate Dehydrogenase Isoenzymes of Saccharomyces
cerevisiae
PURIFICATION, KINETIC PROPERTIES, AND PHYSIOLOGICAL ROLES*
,
-ketoglutarate. The GDH2-encoded
NAD+-dependent glutamate dehydrogenase degrades
glutamate producing ammonium and -ketoglutarate. Until very
recently, it was considered that only one biosynthetic NADP-GDH was
present in S. cerevisiae. This fact hindered understanding
the physiological role of each isoenzyme and the mechanisms involved in
-ketoglutarate channeling for glutamate biosynthesis. In this study,
we purified and characterized the GDH1- and
GDH3-encoded NADP-GDHs; they showed different allosteric properties and rates of -ketoglutarate utilization. Analysis of the
relative levels of these proteins revealed that the expression of
GDH1 and GDH3 is differentially regulated and
depends on the nature of the carbon source. Moreover, the physiological
study of mutants lacking or overexpressing GDH1 or
GDH3 suggested that these genes play nonredundant
physiological roles. Our results indicate that the coordinated
regulation of GDH1-, GDH3-, and GDH2-encoded enzymes results in glutamate biosynthesis and
balanced utilization of -ketoglutarate under fermentative and
respiratory conditions. The possible relevance of the duplicated
NADP-GDH pathway in the adaptation to facultative metabolism is discussed.
*
This work was supported in part by Dirección General
de Asuntos del Personal Académico, Universidad Nacional
Autónoma de México (UNAM), Grant IN212898 and by
Consejo Nacional de Ciencia y Tecnología Grant 31774.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Recipient of a fellowship and a grant (PAEP-102315) from the
Dirección General de Estudios de Posgrado, UNAM.
§
To whom correspondence should be addressed. Tel.: 52-56225631; Fax:
52-56225630; E-mail: amanjarr@ifisiol.unam.mx.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F.-M. Lin, B. Qiao, and Y.-J. Yuan Comparative Proteomic Analysis of Tolerance and Adaptation of Ethanologenic Saccharomyces cerevisiae to Furfural, a Lignocellulosic Inhibitory Compound Appl. Envir. Microbiol., June 1, 2009; 75(11): 3765 - 3776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Vamsee-Krishna and P. S. Phale Carbon source-dependent modulation of NADP-glutamate dehydrogenases in isophthalate-degrading Pseudomonas aeruginosa strain PP4, Pseudomonas strain PPD and Acinetobacter lwoffii strain ISP4 Microbiology, November 1, 2008; 154(11): 3329 - 3337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Quezada, C. Aranda, A. DeLuna, H. Hernandez, M. L. Calcagno, A. Marin-Hernandez, and A. Gonzalez Specialization of the paralogue LYS21 determines lysine biosynthesis under respiratory metabolism in Saccharomyces cerevisiae Microbiology, June 1, 2008; 154(6): 1656 - 1667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Presser, M. B. Elowitz, M. Kellis, and R. Kishony The evolutionary dynamics of the Saccharomyces cerevisiae protein interaction network after duplication PNAS, January 22, 2008; 105(3): 950 - 954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Singh, D. Kumar, N. Ramakrishnan, V. Singhal, J. Jervis, J. F. Garst, S. M. Slaughter, A. M. DeSantis, M. Potts, and R. F. Helm Transcriptional Response of Saccharomyces cerevisiae to Desiccation and Rehydration Appl. Envir. Microbiol., December 1, 2005; 71(12): 8752 - 8763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. N. Vemuri and A. A. Aristidou Metabolic Engineering in the -omics Era: Elucidating and Modulating Regulatory Networks Microbiol. Mol. Biol. Rev., June 1, 2005; 69(2): 197 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Noor and N. S. Punekar Allosteric NADP-glutamate dehydrogenase from aspergilli: purification, characterization and implications for metabolic regulation at the carbon-nitrogen interface Microbiology, May 1, 2005; 151(5): 1409 - 1419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Patil and J. Nielsen Uncovering transcriptional regulation of metabolism by using metabolic network topology PNAS, February 22, 2005; 102(8): 2685 - 2689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Contreras-Shannon, A.-P. Lin, M. T. McCammon, and L. McAlister-Henn Kinetic Properties and Metabolic Contributions of Yeast Mitochondrial and Cytosolic NADP+-specific Isocitrate Dehydrogenases J. Biol. Chem., February 11, 2005; 280(6): 4469 - 4475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Das and P. J. Bhat Disruption of MRG19 results in altered nitrogen metabolic status and defective pseudohyphal development in Saccharomyces cerevisiae Microbiology, January 1, 2005; 151(1): 91 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Magasanik Ammonia Assimilation by Saccharomyces cerevisiae Eukaryot. Cell, October 1, 2003; 2(5): 827 - 829. [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kazuoka, S. Takigawa, N. Arakawa, Y. Hizukuri, I. Muraoka, T. Oikawa, and K. Soda Novel Psychrophilic and Thermolabile L-Threonine Dehydrogenase from Psychrophilic Cytophaga sp. Strain KUC-1 J. Bacteriol., August 1, 2003; 185(15): 4483 - 4489. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |