Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M107696200 on September 25, 2001

J. Biol. Chem., Vol. 276, Issue 48, 44688-44694, November 30, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/48/44688    most recent
M107696200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by You, Y.-H.
Right arrow Articles by Pfeifer, G. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by You, Y.-H.
Right arrow Articles by Pfeifer, G. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Cyclobutane Pyrimidine Dimers Are Responsible for the Vast Majority of Mutations Induced by UVB Irradiation in Mammalian Cells*

Young-Hyun YouDagger §, Dong-Hyun LeeDagger , Jung-Hoon YoonDagger , Satoshi Nakajima, Akira Yasui, and Gerd P. PfeiferDagger ||

From the Dagger  Department of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010 and the  Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-77, Japan

The most prevalent DNA lesions induced by UVB are the cyclobutane pyrimidine dimers (CPDs) and the pyrimidine (6-4) pyrimidone photoproducts ((6-4)PPs). It has been a long standing controversy as to which of these photoproduct is responsible for mutations in mammalian cells. Here we have introduced photoproduct-specific DNA photolyases into a mouse cell line carrying the transgenic mutation reporter genes lacI and cII. Exposure of the photolyase-expressing cell lines to photoreactivating light resulted in almost complete repair of either CPDs or (6-4)PPs within less than 3 h. The mutations produced by the remaining, nonrepaired photoproducts were scored. The mutant frequency in the cII gene after photoreactivation by CPD photolyase was reduced from 127 × 10-5 to 34 × 10-5 (background, 8-10 × 10-5). Photoreactivation with (6-4) photolyase did not lower the mutant frequency appreciably. In the lacI gene the mutant frequency after photoreactivation repair of CPDs was reduced from 148 × 10-5 to 28 × 10-5 (background, 6-10 × 10-5). Mutation spectra obtained with and without photoreactivation by CPD photolyase indicated that the remaining mutations were derived from background mutations, unrepaired CPDs, and other DNA photopoducts including perhaps a small contribution from (6-4)PPs. We conclude that CPDs are responsible for at least 80% of the UVB-induced mutations in this mammalian cell model.

* This work was supported by Grant ES06070 from the NIEHS, National Institutes of Health (to G. P. P.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Present address: Lawrence Berkeley National Laboratory, Berkeley, CA 94720.

|| To whom correspondence should be addressed. Tel.: 626-301-8853; Fax: 626-930-5366; E-mail: gpfeifer@coh.org.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
V. Pages, S. R. Santa Maria, L. Prakash, and S. Prakash
Role of DNA damage-induced replication checkpoint in promoting lesion bypass by translesion synthesis in yeast
Genes & Dev., June 15, 2009; 23(12): 1438 - 1449.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
J. I. Lucas-Lledo and M. Lynch
Evolution of Mutation Rates: Phylogenomic Analysis of the Photolyase/Cryptochrome Family
Mol. Biol. Evol., May 1, 2009; 26(5): 1143 - 1153.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. Liapis, K. I.E. McLuckie, P. D. Lewis, P. B. Farmer, and K. Brown
Mutagenicity of tamoxifen DNA adducts in human endometrial cells and in silico prediction of p53 mutation hotspots
Nucleic Acids Res., October 1, 2008; 36(18): 5933 - 5945.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
P.D. Lewis, B. Manshian, M.N. Routledge, G.B. Scott, and P.A. Burns
Comparison of induced and cancer-associated mutational spectra using multivariate data analysis
Carcinogenesis, April 1, 2008; 29(4): 772 - 778.
[Abstract] [Full Text] [PDF]

Home page
 Hum Exp ToxicolHome page
M. G. Armelini, K. M. Lima-Bessa, M. C. N. Marchetto, A. R. Muotri, V. Chigancas, R. A. Leite, H. Carvalho, and C. F. M. Menck
Exploring DNA damage responses in human cells with recombinant adenoviral vectors
Human and Experimental Toxicology, November 1, 2007; 26(11): 899 - 906.
[Abstract] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Unsal-Kacmaz, P. D. Chastain, P.-P. Qu, P. Minoo, M. Cordeiro-Stone, A. Sancar, and W. K. Kaufmann
The Human Tim/Tipin Complex Coordinates an Intra-S Checkpoint Response to UV That Slows Replication Fork Displacement
Mol. Cell. Biol., April 15, 2007; 27(8): 3131 - 3142.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. J. H. Davies, J. F. Malone, Y. Gan, C. J. Cardin, M. P.H. Lee, and S. Neidle
High-resolution crystal structure of the intramolecular d(TpA) thymine-adenine photoadduct and its mechanistic implications
Nucleic Acids Res., February 28, 2007; 35(4): 1048 - 1053.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Ohkumo, Y. Kondo, M. Yokoi, T. Tsukamoto, A. Yamada, T. Sugimoto, R. Kanao, Y. Higashi, H. Kondoh, M. Tatematsu, et al.
UV-B Radiation Induces Epithelial Tumors in Mice Lacking DNA Polymerase {eta} and Mesenchymal Tumors in Mice Deficient for DNA Polymerase {iota}
Mol. Cell. Biol., October 15, 2006; 26(20): 7696 - 7706.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
P. Kramata, Y.-P. Lu, Y.-R. Lou, J. L. Cohen, M. Olcha, S. Liu, and A. H. Conney
Effect of administration of caffeine or green tea on the mutation profile in the p53 gene in early mutant p53-positive patches of epidermal cells induced by chronic UVB-irradiation of hairless SKH-1 mice
Carcinogenesis, November 1, 2005; 26(11): 1965 - 1974.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Besaratinia, T. W. Synold, H.-H. Chen, C. Chang, B. Xi, A. D. Riggs, and G. P. Pfeifer
DNA lesions induced by UV A1 and B radiation in human cells: Comparative analyses in the overall genome and in the p53 tumor suppressor gene
PNAS, July 19, 2005; 102(29): 10058 - 10063.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. A. Goukassian, E. Helms, H. van Steeg, C. van Oostrom, J. Bhawan, and B. A. Gilchrest
Topical DNA oligonucleotide therapy reduces UV-induced mutations and photocarcinogenesis in hairless mice
PNAS, March 16, 2004; 101(11): 3933 - 3938.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Higurashi, T. Ohtsuki, A. Inase, R. Kusumoto, C. Masutani, F. Hanaoka, and S. Iwai
Identification and Characterization of an Intermediate in the Alkali Degradation of (6-4) Photoproduct-containing DNA
J. Biol. Chem., December 19, 2003; 278(51): 51968 - 51973.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Besaratinia and G. P. Pfeifer
Enhancement of the Mutagenicity of Benzo(a)pyrene Diol Epoxide by a Nonmutagenic Dose of Ultraviolet A Radiation
Cancer Res., December 15, 2003; 63(24): 8708 - 8716.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. E. Fitch, S. Nakajima, A. Yasui, and J. M. Ford
In Vivo Recruitment of XPC to UV-induced Cyclobutane Pyrimidine Dimers by the DDB2 Gene Product
J. Biol. Chem., November 21, 2003; 278(47): 46906 - 46910.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
H. Ikehata, H. Kudo, T. Masuda, and T. Ono
UVA induces C->T transitions at methyl-CpG-associated dipyrimidine sites in mouse skin epidermis more frequently than UVB
Mutagenesis, November 1, 2003; 18(6): 511 - 519.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Mathonnet, C. Leger, J. Desnoyers, R. Drouin, J.-P. Therrien, and E. A. Drobetsky
UV wavelength-dependent regulation of transcription-coupled nucleotide excision repair in p53-deficient human cells
PNAS, June 10, 2003; 100(12): 7219 - 7224.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. J. Rochette, J.-P. Therrien, R. Drouin, D. Perdiz, N. Bastien, E. A. Drobetsky, and E. Sage
UVA-induced cyclobutane pyrimidine dimers form predominantly at thymine-thymine dipyrimidines and correlate with the mutation spectrum in rodent cells
Nucleic Acids Res., June 1, 2003; 31(11): 2786 - 2794.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Stary, P. Kannouche, A. R. Lehmann, and A. Sarasin
Role of DNA Polymerase {eta} in the UV Mutation Spectrum in Human Cells
J. Biol. Chem., May 23, 2003; 278(21): 18767 - 18775.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C.-A. Whittle and M. O. Johnston
Male-biased transmission of deleterious mutations to the progeny in Arabidopsisthaliana
PNAS, April 1, 2003; 100(7): 4055 - 4059.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D.-H. Lee and G. P. Pfeifer
Deamination of 5-Methylcytosines within Cyclobutane Pyrimidine Dimers Is an Important Component of UVB Mutagenesis
J. Biol. Chem., March 21, 2003; 278(12): 10314 - 10321.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
J.-H. Yoon, C.-S. Lee, and G. P. Pfeifer
Simulated sunlight and benzo[a]pyrene diol epoxide induced mutagenesis in the human p53 gene evaluated by the yeast functional assay: lack of correspondence to tumor mutation spectra
Carcinogenesis, January 1, 2003; 24(1): 113 - 119.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
C. Leger and E. A. Drobetsky
Modulation of the DNA damage response in UV-exposed human lymphoblastoid cells through genetic-versus functional-inactivation of the p53 tumor suppressor
Carcinogenesis, October 1, 2002; 23(10): 1631 - 1640.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement