|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 48, 44944-44952, November 30, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Laboratory of Signal Transduction, Molecular Endocrinology
Group, NIEHS, National Institutes of Health,
Research Triangle Park, North Carolina 27709
Activation of protein kinase C (PKC) triggers
cellular signals that inhibit Fas/CD95-induced cell death in Jurkat
T-cells by poorly defined mechanisms. Previously, we have shown that
one effect of PKC on Fas/CD95-dependent cell death occurs
through inhibition of cell shrinkage and K+ efflux
(Gómez-Angelats, M., Bortner, C. D., and Cidlowski, J. A. (2000) J. Biol. Chem. 275, 19609-19619). Here we
report that PKC alters Fas/CD95 signaling from the plasma membrane to
the activation of caspases by exerting a profound action on
survival/cell death decisions. Specific activation of PKC with
12-O-tetradecanoylphorbol-13-acetate or bryostatin-1
induced translocation of PKC from the cytosol to the membrane and
effectively inhibited cell shrinkage and cell death triggered by
anti-Fas antibody in Jurkat cells. In contrast, inhibition of classical
PKC isotypes with Gö6976 exacerbated the effect of Fas activation
on both apoptotic volume decrease and cell death. PKC
activation/inhibition did not affect anti-Fas antibody binding to the
cell surface, intracellular levels of FADD
(Fas-associated protein with death
domain), or c-FLIP (cellular FLICE-like
inhibitory protein) expression. However,
processing/activation of both caspase-8 and caspase-3 and BID cleavage
were markedly blocked upon PKC activation and, conversely, were
augmented during PKC inhibition, suggesting a role for PKC upstream of
caspase-8 processing and activation. Analysis of death-inducing
signaling complex (DISC) formation was carried out to examine the
influence of PKC on recruitment of both FADD and procaspase-8 to the
Fas receptor. PKC activation blocked FADD recruitment and caspase-8 activation and thus DISC formation in both type I and II cells. In
contrast, inhibition of classical PKCs promoted the opposite effect on
the Fas pathway by rapidly increasing FADD recruitment, caspase-8
activation, and DISC formation. Together, these data show that PKC
finely modulates Fas/CD95 signaling by altering the efficiency of DISC formation.
Protein Kinase C Regulates FADD Recruitment and
Death-inducing Signaling Complex Formation in Fas/CD95-induced
Apoptosis*
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Laboratory of Signal
Transduction, Molecular Endocrinology Group, NIEHS, NIH, 111 Alexander
Dr., Research Triangle Park, NC 27709. Tel.: 919-541-1564; Fax:
919-541-1367; E-mail: cidlowski@niehs.nih.gov.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Engedal, P. Auberger, and H. K. Blomhoff Retinoic acid regulates Fas-induced apoptosis in Jurkat T cells: reversal of mitogen-mediated repression of Fas DISC assembly J. Leukoc. Biol., March 1, 2009; 85(3): 469 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-W. Park, H. P. Kim, S.-J. Lee, X. Wang, Y. Wang, E. Ifedigbo, S. C. Watkins, M. Ohba, S. W. Ryter, Y. M. Vyas, et al. Protein Kinase C{alpha} and {zeta} Differentially Regulate Death-Inducing Signaling Complex Formation in Cigarette Smoke Extract-Induced Apoptosis J. Immunol., April 1, 2008; 180(7): 4668 - 4678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Sharif-Askari, D. Gaucher, R. Halwani, J. Ma, K. Jao, A. Abdallah, E. K. Haddad, and R.-P. Sekaly p56Lck Tyrosine Kinase Enhances the Assembly of Death-inducing Signaling Complex during Fas-mediated Apoptosis J. Biol. Chem., December 7, 2007; 282(49): 36048 - 36056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-C. Su, Y.-P. Lin, Y.-J. Cheng, J.-Y. Huang, W.-J. Chuang, Y.-S. Shan, and B.-C. Yang Phosphatidylinositol 3-Kinase/Akt Activation by Integrin-Tumor Matrix Interaction Suppresses Fas-Mediated Apoptosis in T Cells J. Immunol., October 1, 2007; 179(7): 4589 - 4597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. S. Byun, K. A. Park, M. Won, K.-J. Yang, S. Shin, L. Piao, J. Y. Kwak, Z.-W. Lee, J. Park, J. H. Seok, et al. Phorbol 12-Myristate 13-Acetate Protects against Tumor Necrosis Factor (TNF)-Induced Necrotic Cell Death by Modulating the Recruitment of TNF Receptor 1-Associated Death Domain and Receptor-Interacting Protein into the TNF Receptor 1 Signaling Complex: Implication for the Regulatory Role of Protein Kinase C Mol. Pharmacol., September 1, 2006; 70(3): 1099 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cudrici, F. Niculescu, T. Jensen, E. Zafranskaia, M. Fosbrink, V. Rus, M. L. Shin, and H. Rus C5b-9 Terminal Complex Protects Oligodendrocytes from Apoptotic Cell Death by Inhibiting Caspase-8 Processing and Up-Regulating FLIP. J. Immunol., March 1, 2006; 176(5): 3173 - 3180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Wang, Y. Wang, J. Zhang, H. P. Kim, S. W. Ryter, and A. M. K. Choi FLIP Protects against Hypoxia/Reoxygenation-Induced Endothelial Cell Apoptosis by Inhibiting Bax Activation Mol. Cell. Biol., June 1, 2005; 25(11): 4742 - 4751. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gillespie, X. D. Zhang, and P. Hersey Variable expression of protein kinase C{varepsilon} in human melanoma cells regulates sensitivity to TRAIL-induced apoptosis Mol. Cancer Ther., April 1, 2005; 4(4): 668 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. P. Cousens, F. A. Goulette, and J. W. Darnowski JAK-Mediated Signaling Inhibits Fas Ligand-Induced Apoptosis Independent of De Novo Protein Synthesis J. Immunol., January 1, 2005; 174(1): 320 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kotone-Miyahara, K. Yamashita, K.-K. Lee, S. Yonehara, T. Uchiyama, M. Sasada, and A. Takahashi Short-term delay of Fas-stimulated apoptosis by GM-CSF as a result of temporary suppression of FADD recruitment in neutrophils: evidence implicating phosphatidylinositol 3-kinase and MEK1-ERK1/2 pathways downstream of classical protein kinase C J. Leukoc. Biol., November 1, 2004; 76(5): 1047 - 1056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hermoso, P. Olivero, R. Torres, A. Riveros, A. F. G. Quest, and A. Stutzin Cell Volume Regulation in Response to Hypotonicity Is Impaired in HeLa Cells Expressing a Protein Kinase C {alpha} Mutant Lacking Kinase Activity J. Biol. Chem., April 23, 2004; 279(17): 17681 - 17689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takagi, J. Du, X.-Y. Ma, I. Nakashima, and F. Nagase Phorbol 12-Myristate 13-Acetate Protects Jurkat Cells from Methylglyoxal-Induced Apoptosis by Preventing c-Jun N-Terminal Kinase-Mediated Leakage of Cytochrome c in an Extracellular Signal-Regulated Kinase-Dependent Manner Mol. Pharmacol., March 1, 2004; 65(3): 778 - 787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yamauchi, M. Hayashi, S. Abe-Dohmae, and S. Yokoyama Apolipoprotein A-I Activates Protein Kinase C{alpha} Signaling to Phosphorylate and Stabilize ATP Binding Cassette Transporter A1 for the High Density Lipoprotein Assembly J. Biol. Chem., November 28, 2003; 278(48): 47890 - 47897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Harper, M. A. Hughes, S. N. Farrow, G. M. Cohen, and M. MacFarlane Protein Kinase C Modulates Tumor Necrosis Factor-related Apoptosis-inducing Ligand-induced Apoptosis by Targeting the Apical Events of Death Receptor Signaling J. Biol. Chem., November 7, 2003; 278(45): 44338 - 44347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Gnesutta and A. Minden Death Receptor-Induced Activation of Initiator Caspase 8 Is Antagonized by Serine/Threonine Kinase PAK4 Mol. Cell. Biol., November 1, 2003; 23(21): 7838 - 7848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Storey, M. Gomez-Angelats, C. D. Bortner, D. L. Armstrong, and J. A. Cidlowski Stimulation of Kv1.3 Potassium Channels by Death Receptors during Apoptosis in Jurkat T Lymphocytes J. Biol. Chem., August 29, 2003; 278(35): 33319 - 33326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Tillman, K. Izeradjene, K. S. Szucs, L. Douglas, and J. A. Houghton Rottlerin Sensitizes Colon Carcinoma Cells to Tumor Necrosis Factor-related Apoptosis-inducing Ligand-induced Apoptosis via Uncoupling of the Mitochondria Independent of Protein Kinase C Cancer Res., August 15, 2003; 63(16): 5118 - 5125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Engedal and H. K. Blomhoff Combined Action of ERK and NFkappa B Mediates the Protective Effect of Phorbol Ester on Fas-induced Apoptosis in Jurkat Cells J. Biol. Chem., March 21, 2003; 278(13): 10934 - 10941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Miyake, H. Kakeya, T. Kataoka, and H. Osada Epoxycyclohexenone Inhibits Fas-mediated Apoptosis by Blocking Activation of Pro-caspase-8 in the Death-inducing Signaling Complex J. Biol. Chem., March 21, 2003; 278(13): 11213 - 11220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Stupack and D. A. Cheresh Get a ligand, get a life: integrins, signaling and cell survival J. Cell Sci., January 10, 2002; 115(19): 3729 - 3738. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |