|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 48, 45330-45340, November 30, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Institute of Anatomy of the Bayerische
Julius-Maximilians-Universität,
97070 Würzburg, Germany
Previously we cloned RS1, a 67-kDa polypeptide
that is associated with the intracellular side of the plasma membrane.
Upon co-expression in Xenopus laevis oocytes, human RS1
decreased the concentration of the
Na+-D-glucose co-transporter hSGLT1 in the
plasma membrane (Valentin, M., Kühlkamp, T., Wagner, K., Krohne,
G., Arndt, P., Baumgarten, K., Weber, W.-M., Segal, A., Veyhl, M., and
Koepsell, H. (2000) Biochim. Biophys. Acta 1468, 367-380).
Here, the porcine renal epithelial cell line LLC-PK1 was used to
investigate whether porcine RS1 (pRS1) plays a role in transcriptional
up-regulation of SGLT1 after confluence and in down-regulation of SGLT1
by high extracellular D-glucose concentrations. Western
blots indicated a dramatic decrease of endogenous pRS1 protein at the
plasma membrane after confluence but no significant effect of
D-glucose. In confluent LLC-PK1 cells overexpressing pRS1,
SGLT1 mRNA, protein, and methyl-
The Plasma Membrane-associated Protein RS1 Decreases
Transcription of the Transporter SGLT1 in Confluent
LLC-PK1 Cells*
,,
-D-glucopyranoside uptakes were drastically decreased; however, the reduction of methyl--D-glucopyranoside uptake after cultivation with
25 mM D-glucose remained. In confluent pRS1
antisense cells, the expression of SGLT1 mRNA and protein was
strongly increased, whereas the reduction of SGLT1 expression during
cultivation with high D-glucose was not influenced. Nuclear
run-on assays showed that the transcription of SGLT1 was 10-fold
increased in the pRS1 antisense cells. The data suggest that RS1
participates in transcriptional up-regulation of SGLT1 after confluence
but not in down-regulation by D-glucose.
*
This work was supported by the Deutsche
Forschungsgemeinschaft Grant SFB 487/C1.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Both authors contributed equally to this work.
§
To whom correspondence should be addressed: Anatomisches Institut,
Koellikerstr. 6, 97070 Würzburg, Germany. Fax: 0931 312087; E-mail: Hermann@Koepsell.de.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. J. Lee, J. S. Heo, H. N. Suh, M. Y. Lee, and H. J. Han Interleukin-6 stimulates {alpha}-MG uptake in renal proximal tubule cells: involvement of STAT3, PI3K/Akt, MAPKs, and NF-{kappa}B Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1036 - F1046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Vernaleken, M. Veyhl, V. Gorboulev, G. Kottra, D. Palm, B.-C. Burckhardt, G. Burckhardt, R. Pipkorn, N. Beier, C. van Amsterdam, et al. Tripeptides of RS1 (RSC1A1) Inhibit a Monosaccharide-dependent Exocytotic Pathway of Na+-D-Glucose Cotransporter SGLT1 with High Affinity J. Biol. Chem., September 28, 2007; 282(39): 28501 - 28513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kroiss, M. Leyerer, V. Gorboulev, T. Kuhlkamp, H. Kipp, and H. Koepsell Transporter regulator RS1 (RSC1A1) coats the trans-Golgi network and migrates into the nucleus Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1201 - F1212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Veyhl, T. Keller, V. Gorboulev, A. Vernaleken, and H. Koepsell RS1 (RSC1A1) regulates the exocytotic pathway of Na+-D-glucose cotransporter SGLT1 Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1213 - F1223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Jiang, O. Prokopenko, L. Wong, M. Inouye, and O. Mirochnitchenko IRIP, a New Ischemia/Reperfusion-Inducible Protein That Participates in the Regulation of Transporter Activity Mol. Cell. Biol., August 1, 2005; 25(15): 6496 - 6508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Osswald, K. Baumgarten, F. Stumpel, V. Gorboulev, M. Akimjanova, K.-P. Knobeloch, I. Horak, R. Kluge, H.-G. Joost, and H. Koepsell Mice without the Regulator Gene Rsc1A1 Exhibit Increased Na+-D-Glucose Cotransport in Small Intestine and Develop Obesity Mol. Cell. Biol., January 1, 2005; 25(1): 78 - 87. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Bormans, G. Van Oosterwyck, T. J. de Groot, M. Veyhl, L. Mortelmans, A. M. Verbruggen, and H. Koepsell Synthesis and Biologic Evaluation of 11C-Methyl-D-Glucoside, a Tracer of the Sodium-Dependent Glucose Transporters J. Nucl. Med., July 1, 2003; 44(7): 1075 - 1081. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |