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J. Biol. Chem., Vol. 276, Issue 48, 45462-45469, November 30, 2001
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From the Department of Pharmacology, University of Minnesota
Medical School, Minneapolis, Minnesota 55455
Previously, we identified a minimum core promoter
of the mouse
Transcriptional Regulation of Mouse
-Opioid Receptor Gene
ROLE OF Ets-1 IN THE TRANSCRIPTIONAL ACTIVATION OF MOUSE
-OPIOID RECEPTOR GENE*
and-opioid receptor (DOR) gene. The DOR promoter contains
an E-box that binds upstream stimulatory factor and is crucial
for the DOR promoter activity in NS20Y cells, a mouse neuronal cell line that constitutively expresses DOR. In the present study, we
further analyzed the DOR promoter in NS20Y cells and have demonstrated that transcription factor Ets-1 binds to an Ets-1-binding site overlapping the E-box and trans-activates the DOR promoter by synergizing with upstream stimulatory factor in specific DNA binding. In addition, the Ets-1 DNA-binding domain is sufficient to play the
functional role of Ets-1 in trans-activating the DOR promoter. Furthermore, through in vivo cross-linking assays and
Northern blot analyses, we have demonstrated that Ets-1 binds to the
DOR promoter in the neonatal mouse brain and that overexpressed Ets-1 can significantly enhance the expression of DOR mRNA in primary neonatal mouse neuronal cells. Collectively, our data suggest that
Ets-1 functions as a trans-activator of the DOR promoter in the
neonatal mouse brain and thus may contribute to the development of the
mouse brain DOR system.
*
This work was supported by National Institutes of Health
Grants DA-00546, DA-01583, DA-11806, and KO5-DA-70554 and by the A. & F. Stark Fund of the Minnesota Medical Foundation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Pharmacology,
University of Minnesota Medical School, 6-120 Jackson Hall, 321 Church
St. S.E., Minneapolis, MN 55455. Tel.: 612-626-6539; Fax: 612-625-8408;
E-mail: sunx0078@tc.umn.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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