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Originally published In Press as doi:10.1074/jbc.M109247200 on September 26, 2001

J. Biol. Chem., Vol. 276, Issue 49, 45740-45743, December 7, 2001
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RecA Protein Filaments Disassemble in the 5' to 3' Direction on Single-stranded DNA*

Julie M. BorkDagger , Michael M. Cox§, and Ross B. Inman

From the Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706-1544

RecA protein forms filaments on both single- and double-stranded DNA. Several studies confirm that filament extension occurs in the 5' to 3' direction on single-stranded DNA. These filaments also disassemble in an end-dependent fashion, and several indirect observations suggest that the disassembly occurs on the end opposite to that at which assembly occurs. By labeling the 5' end of single-stranded DNA with a segment of duplex DNA, we demonstrate unambiguously that RecA filaments disassemble uniquely in the 5' to 3' direction.

* This work was supported by Grants GM32335 and GM52725 (to M. M. C.) and GM14711 (to R. B. I.) from the National Institutes of Health.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Present address: Laboratory of Molecular Genetics, NIDCD, National Institutes of Health, 5 Research Ct., Rockville, MD 20859.

§ To whom correspondence should be addressed: Dept. of Biochemistry, University of Wisconsin-Madison, 433 Babcock Dr., Madison, WI 53706-1544. Tel.: 608-262-1181; Fax: 608-265-2603; E-mail: cox@biochem.wisc.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.