|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 49, 46196-46203, December 7, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Acidocalcisomes are acidic calcium storage
compartments described initially in trypanosomatid and apicomplexan
parasites. In this work, we describe organelles with properties similar
to acidocalcisomes in the green alga Chlamydomonas
reinhardtii. Nigericin and NH4Cl released
45Ca2+ from preloaded permeabilized cells,
suggesting the incorporation of a significant amount of this cation
into an acidic compartment. X-ray microanalysis of the electron-dense
vacuoles or polyphosphate bodies of C. reinhardtii showed
large amounts of phosphorus, magnesium, calcium, and zinc.
Immunofluorescence microscopy, using antisera raised against a peptide
sequence of the vacuolar type proton pyrophosphatase
(H+-PPase) of Arabidopsis thaliana which is
conserved in the C. reinhardtii enzyme, indicated
localization in the plasma membrane, in intracellular vacuoles, and the
contractile vacuole where it colocalized with the vacuolar proton
ATPase (V-H+-ATPase). Purification of the electron-dense
vacuoles using iodixanol density gradients indicated a preferential
localization of the H+-PPase and the
V-H+-ATPase activities in addition to high concentrations
of PPi and short and long chain polyphosphate, but lack of
markers for mitochondria and chloroplasts. In isolated electron-dense
vacuoles, PPi-driven proton translocation was stimulated by
potassium ions and inhibited by the PPi analog
aminomethylenediphosphonate. Potassium fluoride, imidodiphosphate,
N,N'-dicyclohexylcarbodiimide, and
N-ethylmaleimide also inhibited PPi hydrolysis
in the isolated organelles in a dose-dependent manner.
These results indicate that the electron-dense vacuoles of C. reinhardtii are very similar to acidocalcisomes with regard to
their chemical composition and the presence of proton pumps.
Polyphosphate was also localized to the contractile vacuole by
4',6-diamidino-2-phenylindole staining, suggesting, with the
immunochemical data, a link between these organelles and the acidocalcisomes.
The Polyphosphate Bodies of Chlamydomonas
reinhardtii Possess a Proton-pumping Pyrophosphatase and Are
Similar to Acidocalcisomes*
,,§,¶
Laboratory of Molecular Parasitology,
Department of Pathobiology, and the § Department of Plant
Biology, University of Illinois at Urbana-Champaign,
Urbana, Illinois 61802
*
This work was supported in part by National Institutes of
Health Grant AI-23259 (to R. D.) and National Science Foundation Photosynthesis Training Grant DBI 96-02240 (to G.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. K. Pittman, C. Edmond, P. A. Sunderland, and C. M. Bray A Cation-regulated and Proton Gradient-dependent Cation Transporter from Chlamydomonas reinhardtii Has a Role in Calcium and Sodium Homeostasis J. Biol. Chem., January 2, 2009; 284(1): 525 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Paz, E. Shimoni, M. Weiss, and U. Pick Effects of Iron Deficiency on Iron Binding and Internalization into Acidic Vacuoles in Dunaliella salina Plant Physiology, July 1, 2007; 144(3): 1407 - 1415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Au, R. D. Barabote, K. Y. Hu, and M. H. Saier Jr Evolutionary appearance of H+-translocating pyrophosphatases. Microbiology, May 1, 2006; 152(Pt 5): 1243 - 1247. [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Moseley, C.-W. Chang, and A. R. Grossman Genome-Based Approaches to Understanding Phosphorus Deprivation Responses and PSR1 Control in Chlamydomonas reinhardtii Eukaryot. Cell, January 1, 2006; 5(1): 26 - 44. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bolling and O. Fiehn Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation Plant Physiology, December 1, 2005; 139(4): 1995 - 2005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Saito, R. Ohtomo, Y. Kuga-Uetake, T. Aono, and M. Saito Direct Labeling of Polyphosphate at the Ultrastructural Level in Saccharomyces cerevisiae by Using the Affinity of the Polyphosphate Binding Domain of Escherichia coli Exopolyphosphatase Appl. Envir. Microbiol., October 1, 2005; 71(10): 5692 - 5701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhang, M. R. Gomez-Garcia, M. R. W. Brown, and A. Kornberg Inorganic polyphosphate in Dictyostelium discoideum: Influence on development, sporulation, and predation PNAS, February 22, 2005; 102(8): 2731 - 2735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Rohloff, A. Montalvetti, and R. Docampo Acidocalcisomes and the Contractile Vacuole Complex Are Involved in Osmoregulation in Trypanosoma cruzi J. Biol. Chem., December 10, 2004; 279(50): 52270 - 52281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Seufferheld, C. R. Lea, M. Vieira, E. Oldfield, and R. Docampo The H+-pyrophosphatase of Rhodospirillum rubrum Is Predominantly Located in Polyphosphate-rich Acidocalcisomes J. Biol. Chem., December 3, 2004; 279(49): 51193 - 51202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Gomez-Garcia and A. Kornberg Formation of an actin-like filament concurrent with the enzymatic synthesis of inorganic polyphosphate PNAS, November 9, 2004; 101(45): 15876 - 15880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Ruiz, C. R. Lea, E. Oldfield, and R. Docampo Human Platelet Dense Granules Contain Polyphosphate and Are Similar to Acidocalcisomes of Bacteria and Unicellular Eukaryotes J. Biol. Chem., October 22, 2004; 279(43): 44250 - 44257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Montalvetti, P. Rohloff, and R. Docampo A Functional Aquaporin Co-Localizes with the Vacuolar Proton Pyrophosphatase to Acidocalcisomes and the Contractile Vacuole Complex of Trypanosoma cruzi J. Biol. Chem., September 10, 2004; 279(37): 38673 - 38682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Mimura, Y. Nakanishi, M. Hirono, and M. Maeshima Membrane Topology of the H+-pyrophosphatase of Streptomyces coelicolor Determined by Cysteine-scanning Mutagenesis J. Biol. Chem., August 13, 2004; 279(33): 35106 - 35112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Nakanishi, I. Yabe, and M. Maeshima Patch Clamp Analysis of a H+ Pump Heterologously Expressed in Giant Yeast Vacuoles J. Biochem., October 1, 2003; 134(4): 615 - 623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Seufferheld, M. C. F. Vieira, F. A. Ruiz, C. O. Rodrigues, S. N. J. Moreno, and R. Docampo Identification of Organelles in Bacteria Similar to Acidocalcisomes of Unicellular Eukaryotes J. Biol. Chem., August 8, 2003; 278(32): 29971 - 29978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Drozdowicz, M. Shaw, M. Nishi, B. Striepen, H. A. Liwinski, D. S. Roos, and P. A. Rea Isolation and Characterization of TgVP1, a Type I Vacuolar H+-translocating Pyrophosphatase from Toxoplasma gondii. THE DYNAMICS OF ITS SUBCELLULAR LOCALIZATION AND THE CELLULAR EFFECTS OF A DIPHOSPHONATE INHIBITOR J. Biol. Chem., January 3, 2003; 278(2): 1075 - 1085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. O. Rodrigues, F. A. Ruiz, M. Vieira, J. E. Hill, and R. Docampo An Acidocalcisomal Exopolyphosphatase from Leishmania major with High Affinity for Short Chain Polyphosphate J. Biol. Chem., December 20, 2002; 277(52): 50899 - 50906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. O. Rodrigues, F. A. Ruiz, P. Rohloff, D. A. Scott, and S. N. J. Moreno Characterization of Isolated Acidocalcisomes from Toxoplasma gondii Tachyzoites Reveals a Novel Pool of Hydrolyzable Polyphosphate J. Biol. Chem., December 6, 2002; 277(50): 48650 - 48656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Lemercier, S. Dutoya, S. Luo, F. A. Ruiz, C. O. Rodrigues, T. Baltz, R. Docampo, and N. Bakalara A Vacuolar-type H+-Pyrophosphatase Governs Maintenance of Functional Acidocalcisomes and Growth of the Insect and Mammalian Forms of Trypanosoma brucei J. Biol. Chem., September 27, 2002; 277(40): 37369 - 37376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Belogurov, M. V. Turkina, A. Penttinen, S. Huopalahti, A. A. Baykov, and R. Lahti H+-Pyrophosphatase of Rhodospirillum rubrum. HIGH YIELD EXPRESSION IN ESCHERICHIA COLI AND IDENTIFICATION OF THE CYS RESIDUES RESPONSIBLE FOR INACTIVATION BY MERSALYL J. Biol. Chem., June 14, 2002; 277(25): 22209 - 22214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. McConville, K. A. Mullin, S. C. Ilgoutz, and R. D. Teasdale Secretory Pathway of Trypanosomatid Parasites Microbiol. Mol. Biol. Rev., March 1, 2002; 66(1): 122 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Marchesini, F. A. Ruiz, M. Vieira, and R. Docampo Acidocalcisomes Are Functionally Linked to the Contractile Vacuole of Dictyostelium discoideum J. Biol. Chem., March 1, 2002; 277(10): 8146 - 8153. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |