|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 49, 46284-46289, December 7, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Programmed cell death (PCD) in many systems is
controlled by relative amounts of the apoptosis-regulating proteins Bax
and Bcl-2 through homo- or heterodimerization. Here we show that
Bax-induced PCD of yeast was suppressed by transformation with a
vesicle-associated membrane protein from Arabidopsis
(AtVAMP), which was isolated by screening a cDNA expression library
against sugar-induced cell death in yeast. AtVAMP expression blocked
Bax-induced PCD downstream of oxidative burst. AtVAMP also prevented
H2O2-induced apoptosis in yeast and in
Arabidopsis cells. Reduced oxidation of lipids and plasma
membrane proteins was detected in the AtVAMP-transformed yeast,
suggesting improved membrane repair. Inhibition of intracellular vesicle trafficking by brefeldin A induced apoptosis from a sublethal concentration of H2O2. No protection occurred
by overexpression of the yeast homolog SCN2. However, efficient
suppression of yeast PCD occurred by expression of a chimeric
gene, composed of the conserved domains from yeast, fused to the
variable N-terminal domain from Arabidopsis, resulting in
exchange of the proline-rich N-terminal domain of SCN2 with a
proline-poor Arabidopsis sequence. Our results suggest that
intracellular vesicle traffic can regulate execution of apoptosis by
affecting the rate of membrane recycling and that the proline-rich
N-terminal domain of VAMP inhibited this process.
Vesicle-associated Membrane Protein of
Arabidopsis Suppresses Bax-induced Apoptosis in Yeast
Downstream of Oxidative Burst*
§¶,§,
, and
Department of Plant Sciences, The Hebrew
University of Jerusalem, Givat-Ram, Jerusalem 91904, Israel and the
Institute of Field and Garden Crops, Agricultural Research
Organization, The Volcani Center, Bet-Dagan 50250, Israel
*
This work was supported by grants from The Israel Science
Foundation and Ministry of Science (to A. L. and D. G.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Yang, S. Yang, Y. Li, and J. Hua The Arabidopsis BAP1 and BAP2 Genes Are General Inhibitors of Programmed Cell Death Plant Physiology, September 1, 2007; 145(1): 135 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Leshem, N. Melamed-Book, O. Cagnac, G. Ronen, Y. Nishri, M. Solomon, G. Cohen, and A. Levine Suppression of Arabidopsis vesicle-SNARE expression inhibited fusion of H2O2-containing vesicles with tonoplast and increased salt tolerance PNAS, November 21, 2006; 103(47): 18008 - 18013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kawai-Yamada, Y. Saito, L. Jin, T. Ogawa, K.-M. Kim, L.-H. Yu, Y. Tone, A. Hirata, M. Umeda, and H. Uchimiya A Novel Arabidopsis Gene Causes Bax-like Lethality in Saccharomyces cerevisiae J. Biol. Chem., November 25, 2005; 280(47): 39468 - 39473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mazel, Y. Leshem, B. S. Tiwari, and A. Levine Induction of Salt and Osmotic Stress Tolerance by Overexpression of an Intracellular Vesicle Trafficking Protein AtRab7 (AtRabG3e) Plant Physiology, January 1, 2004; 134(1): 118 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Vandenabeele, K. Van Der Kelen, J. Dat, I. Gadjev, T. Boonefaes, S. Morsa, P. Rottiers, L. Slooten, M. Van Montagu, M. Zabeau, et al. A comprehensive analysis of hydrogen peroxide-induced gene expression in tobacco PNAS, December 23, 2003; 100(26): 16113 - 16118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Wick, X. Gansel, C. Oulevey, V. Page, I. Studer, M. Durst, and L. Sticher The Expression of the t-SNARE AtSNAP33 Is Induced by Pathogens and Mechanical Stimulation Plant Physiology, May 1, 2003; 132(1): 343 - 351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mazzoni, P. Mancini, L. Verdone, F. Madeo, A. Serafini, E. Herker, and C. Falcone A Truncated Form of KlLsm4p and the Absence of Factors Involved in mRNA Decapping Trigger Apoptosis in Yeast Mol. Biol. Cell, February 1, 2003; 14(2): 721 - 729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Ludovico, F. Rodrigues, A. Almeida, M. T. Silva, A. Barrientos, and M. Corte-Real Cytochrome c Release and Mitochondria Involvement in Programmed Cell Death Induced by Acetic Acid in Saccharomyces cerevisiae Mol. Biol. Cell, August 1, 2002; 13(8): 2598 - 2606. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |