HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 5, 3476-3483, February 2, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/5/3476    most recent M009236200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by McCormac, D. J. Articles by Berry, J. O. Search for Related Content PubMed PubMed Citation Articles by McCormac, D. J. Articles by Berry, J. O. Social Bookmarking                      What's this?

Light-associated and Processing-dependent Protein Binding to 5' Regions of rbcL mRNA in the Chloroplasts of a C₄ Plant^*

Dennis J. McCormac, Hanz Litz, Jianxin Wang, Paul D. Gollnick, and James O. Berry^§

From the Department of Biological Sciences, State University of New York, Buffalo, New York 14260

In amaranth, a C₄ dicotyledonous plant, the plastid rbcL gene (encoding the large subunit of ribulose-1,5-bisphosphate carboxylase) is regulated post-transcriptionally during many developmental processes, including light-mediated development. To identify post-transcriptional regulators of rbcL expression, three types of analyses (polysome heel printing, gel retardation, and UV cross-linking) were utilized. These approaches revealed that multiple proteins interact with 5' regions of rbcL mRNA in light-grown, but not etiolated, amaranth plants. Light-associated binding of a 47-kDa protein (p47), observed by UV cross-linking, was highly specific for the rbcL 5' RNA. Binding of p47 occurred only with RNAs corresponding to mature processed rbcL transcripts (5'-untranslated region (UTR) terminating at 66); transcripts with longer 5'-UTRs did not associate with p47 in vitro. Variations in the length of the rbcL 5'-UTR were found to occur in vivo, and these different 5' termini may prevent or enhance light-associated p47 binding, possibly affecting rbcL expression as well. p47 binding correlates with light-dependent rbcL polysome association of the fully processed transcripts in photosynthetic leaves and cotyledons but not with cell-specific rbcL mRNA accumulation in bundle sheath and mesophyll chloroplasts.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. Patel and J. O. Berry Rubisco gene expression in C4 plants J. Exp. Bot., May 1, 2008; 59(7): 1625 - 1634. [Abstract] [Full Text] [PDF]

				C. P. Randle and A. D. Wolfe The evolution and expression of RBCL in holoparasitic sister-genera Harveya and Hyobanche (Orobanchaceae) Am. J. Botany, September 1, 2005; 92(9): 1575 - 1585. [Abstract] [Full Text] [PDF]

				Y. S. Katz and A. Danon The 3'-Untranslated Region of Chloroplast psbA mRNA Stabilizes Binding of Regulatory Proteins to the Leader of the Message J. Biol. Chem., May 17, 2002; 277(21): 18665 - 18669. [Abstract] [Full Text] [PDF]

				Y. Shen, A. Danon, and D. A. Christopher RNA Binding-Proteins Interact Specifically with the Arabidopsis Chloroplast psbA mRNA 5' Untranslated Region in a Redox-Dependent Manner Plant Cell Physiol., October 1, 2001; 42(10): 1071 - 1078. [Abstract] [Full Text] [PDF]