JBC Transcription and Nuclear Factor Monoclonals

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M006662200 on October 31, 2000

J. Biol. Chem., Vol. 276, Issue 5, 3564-3573, February 2, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/5/3564    most recent
M006662200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Salvini, R.
Right arrow Articles by Trinchera, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salvini, R.
Right arrow Articles by Trinchera, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

beta 1,3-Galactosyltransferase beta 3Gal-T5 Acts on the GlcNAcbeta 1right-arrow 3Galbeta 1right-arrow 4GlcNAcbeta 1right-arrow R Sugar Chains of Carcinoembryonic Antigen and Other N-Linked Glycoproteins and Is Down-regulated in Colon Adenocarcinomas*

Roberta Salvini, Anna Bardoni, Maurizia Valli, and Marco TrincheraDagger

From the Department of Biochemistry, University of Pavia, via Taramelli 3B, 27100 Pavia, Italy

We attempted to determine whether beta 1,3-galactosyltransferase beta 3Gal-T5 is involved in the biosynthesis of a specific subset of type 1 chain carbohydrates and expressed in a cancer-associated manner. We transfected Chinese hamster ovary (CHO) cells expressing Fuc-TIII with beta 3Gal-T cDNAs and studied the relevant glycoconjugates formed. beta 3Gal-T5 directs synthesis of Lewis type 1 antigens in CHO cells more efficiently than beta 3Gal-T1, whereas beta 3Gal-T2, -T3, and -T4 are almost unable to direct synthesis. In the clone expressing Fuc-TIII and beta 3Gal-T5 (CHO-FT-T5), sialyl-Lewis a synthesis is strongly inhibited by swainsonine but not by benzyl-alpha -GalNAc, and sialyl-Lewis x is absent, although it is detected in the clones expressing Fuc-TIII and beta 3Gal-T1 (CHO-FT-T1) or Fuc-TIII and beta 3Gal-T2 (CHO-FT-T2). Endo-beta -galactosidase treatment of N- glycans prepared from clone CHO-FT-T5 releases (±NeuAcalpha 2right-arrow3)Galbeta 1right-arrow3[Fucalpha 1right-arrow4]GlcNAcbeta 1right-arrow3Gal but not GlcNAcbeta 1right-arrow3Gal or type 2 chain oligosaccharides, which are found in CHO-FT-T1 cells. This result indicates that beta 3Gal-T5 expression prevents poly-N-acetyllactosamine and sialyl-Lewis x synthesis on N-glycans. Kinetic studies confirm that beta 3Gal-T5 prefers acceptors having the GlcNAcbeta 1right-arrow3Gal end, including lactotriosylceramide. Competitive reverse transcriptase mediated-polymerase chain reaction shows that the beta 3Gal-T5 transcript is expressed in normal colon mucosa but not or poorly in adenocarcinomas. Moreover, recombinant carcinoembryonic antigen purified from a CHO clone expressing Fuc-TIII and beta 3Gal-T5 reacts with anti-sialyl-Lewis a and carries type 1 chains on oligosaccharides released by endo-beta -galactosidase. We conclude that beta 3Gal-T5 down-regulation plays a relevant role in determining the cancer-associated glycosylation pattern of N-glycans.

* This work was supported in part by a grant from the Associazione Italiana Ricerca sul Cancro (to M. T.) and by a grant from the University of Insubria (FAR 1999).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger A researcher at the University of Insubria Medical School (formerly University of Pavia Medical School II). To whom correspondence should be addressed. Tel.: 39-0382-507-233; Fax: 39-0382-423-108; E-mail: dbioc@unipv.it.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
L. Mare and M. Trinchera
Comparative Analysis of Retroviral and Native Promoters Driving Expression of beta1,3-Galactosyltransferase beta3Gal-T5 in Human and Mouse Tissues
J. Biol. Chem., January 5, 2007; 282(1): 49 - 57.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
J. Holgersson and J. Lofling
Glycosyltransferases involved in type 1 chain and Lewis antigen biosynthesis exhibit glycan and core chain specificity
Glycobiology, July 1, 2006; 16(7): 584 - 593.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Miyazaki, K. Ohmori, M. Izawa, T. Koike, K. Kumamoto, K. Furukawa, T. Ando, M. Kiso, T. Yamaji, Y. Hashimoto, et al.
Loss of Disialyl Lewisa, the Ligand for Lymphocyte Inhibitory Receptor Sialic Acid-Binding Immunoglobulin-Like Lectin-7 (Siglec-7) Associated with Increased Sialyl Lewisa Expression on Human Colon Cancers
Cancer Res., July 1, 2004; 64(13): 4498 - 4505.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. A. Dunn, P. Medstrand, and D. L. Mager
An endogenous retroviral long terminal repeat is the dominant promoter for human {beta}1,3-galactosyltransferase 5 in the colon
PNAS, October 28, 2003; 100(22): 12841 - 12846.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Bai, D. Zhou, J. R. Brown, B. E. Crawford, T. Hennet, and J. D. Esko
Biosynthesis of the Linkage Region of Glycosaminoglycans. CLONING AND ACTIVITY OF GALACTOSYLTRANSFERASE II, THE SIXTH MEMBER OF THE beta 1,3-GALACTOSYLTRANSFERASE FAMILY (beta 3GalT6)
J. Biol. Chem., December 14, 2001; 276(51): 48189 - 48195.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Ikeda, H. Eguchi, S. Nishihara, H. Narimatsu, R. Kannagi, T. Irimura, M. Ohta, H. Matsuda, N. Taniguchi, and K. Honke
A Remodeling System of the 3'-Sulfo-Lewis a and 3'-Sulfo-Lewis x Epitopes
J. Biol. Chem., October 12, 2001; 276(42): 38588 - 38594.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.