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Originally published In Press as doi:10.1074/jbc.C100567200 on October 17, 2001

J. Biol. Chem., Vol. 276, Issue 50, 46693-46696, December 14, 2001
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ACCELERATED PUBLICATION
Identification of a Conserved Archaeal RNA Polymerase Subunit Contacted by the Basal Transcription Factor TFB*

Christine P. MagillDagger , Stephen P. JacksonDagger , and Stephen D. Bell§

From the Dagger  Wellcome Trust and Cancer Research Campaign Institute of Cancer and Developmental Biology, Tennis Court Road, Cambridge CB2 1QR, United Kingdom, the § Medical Research Council (MRC) Cancer Cell Unit, Hutchison/MRC Research Center, Hills Road, Cambridge CB2 2QH, United Kingdom, and the Department of Zoology, University of Cambridge, Downing Street, Cambridge CB3 3EJ, United Kingdom

Archaea possess two general transcription factors that are required to recruit RNA polymerase (RNAP) to promoters in vitro. These are TBP, the TATA-box-binding protein and TFB, the archaeal homologue of TFIIB. Thus, the archaeal and eucaryal transcription machineries are fundamentally related. In both RNAP II and archaeal transcription systems, direct contacts between TFB/TFIIB and the RNAP have been demonstrated to mediate recruitment of the polymerase to the promoter. However the subunit(s) directly contacted by these factors has not been identified. Using systematic yeast two-hybrid and biochemical analyses we have identified an interaction between the N-terminal domain of TFB and an evolutionarily conserved subunit of the RNA polymerase, RpoK. Intriguingly, homologues of RpoK are found in all three nuclear RNA polymerases (Rpb6) and also in the bacterial RNA polymerase (omega -subunit).

* This work was supported by the Medical Research Council and the Cancer Research Campaign.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: MRC Cancer Cell Unit, Hutchison/MRC Center, Hills Rd., Cambridge, CB2 2QH, UK. Tel.: 1223-763311; Fax: 1223-763241; E-mail: sdb@mole.bio.cam.ac.uk.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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