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Originally published In Press as doi:10.1074/jbc.M106227200 on October 15, 2001

J. Biol. Chem., Vol. 276, Issue 51, 48398-48403, December 21, 2001
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Exchange of the Actin-bound Nucleotide in Intact Arterial Smooth Muscle*

Michael BárányDagger §, John T. Barron, Liping Gu, and Kate Bárány||

From the Dagger  Department of Biochemistry and Molecular Biology, and || Department of Physiology and Biophysics, University of Illinois College of Medicine, Chicago, Illinois 60612, the  Section of Cardiology, Rush Medical College, Department of Medicine, Chicago, Illinois 60612

The actin-bound ADP was separated from cytoplasmic nucleotides by treatment of intact arterial smooth muscle with 50% ethanol. In 32P-labeled smooth muscle the actin-bound ADP and phosphate readily exchanged with the cytoplasmic [gamma ,beta -32P]ATP; the specific radioactivity of actin-bound ADP was equal to that of the beta -phosphate of cytoplasmic ATP and the specific radioactivity of actin-bound phosphate was equal to that of the gamma -phosphate of cytoplasmic ATP. In contrast, the exchange of the actin-bound ADP in skeletal muscle was very slow. The presence of cytoplasmic ATP was required for the exchange of the actin-bound ADP and phosphate; if ATP synthesis was inhibited the exchange was also inhibited. The extent of exchange was reduced in muscles contracted by histamine or K+, as compared with resting muscles. The exchange was also shown in other mammalian smooth muscles, uterus, urinary bladder, and stomach. The data indicate a dynamic state of actin in smooth muscle. The data also suggest that polymerization-depolymerization of actin is part of the contraction-relaxation cycle of smooth muscle.

* This work was supported by the Edgar Folk Jr. Foundation for Senior Physiologists.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed. Tel.: 312-996-7268; Fax: 312-413-0364; E-mail: mbarany@uic.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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