![]()
|
|
||||||||
J. Biol. Chem., Vol. 276, Issue 52, 49449-49458, December 28, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
,
,
,
,
§,
, and
**
From the The Agrobacterium tumefaciens
quorum-sensing transcriptional regulator TraR and its inducing ligand
3-oxo-octanoyl-L-homoserine lactone control conjugal
transfer of the tumor-inducing plasmid, the primary virulence factor
responsible for crown gall disease of plants. This regulatory system
enables A. tumefaciens to express its conjugal transfer
regulon preferentially at high population densities. TraR activity is
antagonized by a second tumor-inducing plasmid-encoded protein
designated TraM. TraM and TraR are thought to form an anti-activation
complex that prevents TraR from recognizing its target DNA-binding
sites. The formation and inhibitory function of the TraM-TraR
anti-activation complex was analyzed using several different assays for
protein-protein interaction, including surface plasmon resonance. The
TraR-TraM complex forms readily in solution and is extremely stable
(KD of 1-4 × 10
Department of Biology, Indiana University,
Bloomington, Indiana 47405 and ¶ Section of Microbiology,
Cornell University, Ithaca, New York 14853
9
M). Directed mutational analysis of TraM identified a
number of amino acids that play important roles in the inhibition of TraR, clustering in two regions of the protein. Interestingly, several
mutants were identified that proficiently bound TraR but were unable to
inhibit its activity. This observation suggests a mechanistic
separation between the initial assembly of the complex and conversion
of TraR to an inactive form.
Current address: Dept. of Microbiology and Molecular Genetics,
Harvard Medical School, Bldg. D1, 200 Longwood Ave., Boston, MA 02115.
**
To whom correspondence should be addressed: Dept. of Biology,
Jordan Hall 142, 1001 E. 3rd St., Indiana University, Bloomington, IN
47405-1847. Tel.: 812-856-6005; Fax: 812-855-6705; E-mail: cfuqua@bio.indiana.edu.
This article has been cited by other articles:
![]() |
S. Su, S. R. Khan, and S. K. Farrand Induction and Loss of Ti Plasmid Conjugative Competence in Response to the Acyl-Homoserine Lactone Quorum-Sensing Signal J. Bacteriol., July 1, 2008; 190(13): 4398 - 4407. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Kawaguchi, Y. P. Chen, R. S. Norman, and A. W. Decho Rapid Screening of Quorum-Sensing Signal N-Acyl Homoserine Lactones by an In Vitro Cell-Free Assay Appl. Envir. Microbiol., June 15, 2008; 74(12): 3667 - 3671. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Chen, P. D. Jeffrey, C. Fuqua, Y. Shi, and L. Chen Structural basis for antiactivation in bacterial quorum sensing PNAS, October 16, 2007; 104(42): 16474 - 16479. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Qin, S. Su, and S. K. Farrand Molecular Basis of Transcriptional Antiactivation: TraM DISRUPTS THE TraR-DNA COMPLEX THROUGH STEPWISE INTERACTIONS J. Biol. Chem., July 6, 2007; 282(27): 19979 - 19991. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. E. Gonzalez and N. D. Keshavan Messing with Bacterial Quorum Sensing Microbiol. Mol. Biol. Rev., December 1, 2006; 70(4): 859 - 875. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Chen, C. Wang, C. Fuqua, L.-H. Zhang, and L. Chen Crystal Structure and Mechanism of TraM2, a Second Quorum-Sensing Antiactivator of Agrobacterium tumefaciens Strain A6 J. Bacteriol., December 1, 2006; 188(23): 8244 - 8251. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Wang, H.-B. Zhang, G. Chen, L. Chen, and L.-H. Zhang Dual Control of Quorum Sensing by Two TraM-Type Antiactivators in Agrobacterium tumefaciens Octopine Strain A6. J. Bacteriol., April 1, 2006; 188(7): 2435 - 2445. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Cho and S. C. Winans VirA and VirG activate the Ti plasmid repABC operon, elevating plasmid copy number in response to wound-released chemical signals PNAS, October 11, 2005; 102(41): 14843 - 14848. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Vannini, C. Volpari, and S. Di Marco Crystal Structure of the Quorum-sensing Protein TraM and Its Interaction with the Transcriptional Regulator TraR J. Biol. Chem., June 4, 2004; 279(23): 24291 - 24296. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. E. Gonzalez and M. M. Marketon Quorum Sensing in Nitrogen-Fixing Rhizobia Microbiol. Mol. Biol. Rev., December 1, 2003; 67(4): 574 - 592. [Abstract] [Full Text] [PDF] |
||||
![]() |
Z.-Q. Luo, S. Su, and S. K. Farrand In Situ Activation of the Quorum-Sensing Transcription Factor TraR by Cognate and Noncognate Acyl-Homoserine Lactone Ligands: Kinetics and Consequences J. Bacteriol., October 1, 2003; 185(19): 5665 - 5672. [Abstract] [Full Text] [PDF] |
||||
![]() |
X. He, W. Chang, D. L. Pierce, L. O. Seib, J. Wagner, and C. Fuqua Quorum Sensing in Rhizobium sp. Strain NGR234 Regulates Conjugal Transfer (tra) Gene Expression and Influences Growth Rate J. Bacteriol., February 1, 2003; 185(3): 809 - 822. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |