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Originally published In Press as doi:10.1074/jbc.M008166200 on November 14, 2000

J. Biol. Chem., Vol. 276, Issue 6, 3937-3940, February 9, 2001
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Chloroplast RNA Editing Required for Functional Acetyl-CoA Carboxylase in Plants*

Yukiko SasakiDagger , Akiko Kozaki, Akira Ohmori, Hiro Iguchi, and Yukio Nagano

From the Laboratory of Plant Molecular Biology, Graduate School of Agricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

RNA editing is an important post-transcriptional process in chloroplasts and is thought to be functionally significant. Here we show a requirement of RNA editing for a functional enzyme. In peas, acetyl-CoA carboxylase (ACCase), a key enzyme of fatty acid synthesis, is composed of biotin carboxylase with the biotin carboxyl carrier protein and carboxyltransferase (CT). CT is composed of the nuclear-encoded alpha  polypeptide and the chloroplast-encoded beta  polypeptide in peas. One nucleotide of the beta  polypeptide mRNA, which is edited in pea chloroplasts, converts the serine codon to the leucine codon. We show that this RNA editing is required for functional CT by comparing the unedited and edited recombinant enzymes. In plants not having a leucine codon at the same position, editing was shown to take place so as to create the leucine codon, indicating that editing is needed for in vivo CT activity and therefore for ACCase. To our knowledge, ACCase is an essential enzyme, suggesting that the chloroplast RNA editing is necessary for these plants.

* This work was supported by Grand-in-aid 12025211 for Scientific Research in Priority Areas, by Japanese Ministry of Education, Science, Sports, and Culture Grant 10460147, and by Japan Society for the Promotion of Science, Research for Future Program Grants JSPS-RIFT 96L006012 and JSPS-RIFT 97R16001.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Laboratory of Plant Molecular Biology, Graduate School of Agricultural Sciences, Nagoya University, Nagoya 464-8601, Japan. Tel.: 81-52-789-4165; Fax: 81-52-789-4296; E-mail: sasaki@agr.nagoya-u.ac.jp.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


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