|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 6, 3947-3951, February 9, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the We have inactivated the nuclear gene coding for a
putative NAD(P)H dehydrogenase from the inner membrane of
Neurospora crassa mitochondria by repeat-induced point
mutations. The respiratory rates of mitochondria from the resulting
mutant (nde-1) were measured, using NADH or NADPH as
substrates under different assay conditions. The results showed that
the mutant lacks an external calcium-dependent NADPH
dehydrogenase. The observation of NADH and NADPH oxidation by intact
mitochondria from the nde-1 mutant suggests the existence of a second external NAD(P)H dehydrogenase. The topology of the NDE1
protein was further studied by protease accessibility, in vitro import experiments, and in silico analysis of
the amino acid sequence. Taken together, it appears that most of the
NDE1 protein extends into the intermembrane space in a tightly folded conformation and that it remains anchored to the inner mitochondrial membrane by an N-terminal transmembrane domain.
The External Calcium-dependent NADPH Dehydrogenase
from Neurospora crassa Mitochondria*
,,
,**,, and**
Instituto de Biologia Molecular e Celular,
Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto,
Portugal, the § Department of Plant Physiology, Lund
University, Box 117, S-22100 Lund, Sweden, the ¶ Institut
für Physiologische Chemie der Universität München,
Goethestrasse 33, D-80336 München, Germany, the
Department of Biology, University of New Mexico, Albuquerque,
New Mexico 87131, and the ** Instituto de Ciências
Biomédicas de Abel Salazar, Universidade do Porto, 4099-003 Porto, Portugal
*
This research was supported by Fundação para a
Ciência e a Tecnologia from Portugal through research grants (to
A. V.) and a fellowship (to A. M. P. M.), by grants from the
Swedish Natural Science Research Council (to I. M. M.), and by
National Science Foundation Grant MCB-9874488 (to M. A. N.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.:
351-226074900; Fax: 351-226099157; E-mail: asvideir@icbas.up.pt.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y.-J. Liu, A. Nunes-Nesi, S. V. Wallstrom, I. Lager, A. M. Michalecka, F. E.B. Norberg, S. Widell, K. M. Fredlund, A. R. Fernie, and A. G. Rasmusson A Redox-Mediated Modulation of Stem Bolting in Transgenic Nicotiana sylvestris Differentially Expressing the External Mitochondrial NADPH Dehydrogenase Plant Physiology, July 1, 2009; 150(3): 1248 - 1259. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Desplats, F. Mus, S. Cuine, E. Billon, L. Cournac, and G. Peltier Characterization of Nda2, a Plastoquinone-reducing Type II NAD(P)H Dehydrogenase in Chlamydomonas Chloroplasts J. Biol. Chem., February 13, 2009; 284(7): 4148 - 4157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Geisler, C. Broselid, L. Hederstedt, and A. G. Rasmusson Ca2+-binding and Ca2+-independent Respiratory NADH and NADPH Dehydrogenases of Arabidopsis thaliana J. Biol. Chem., September 28, 2007; 282(39): 28455 - 28464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Satrustegui, B. Pardo, and A. del Arco Mitochondrial Transporters as Novel Targets for Intracellular Calcium Signaling Physiol Rev, January 1, 2007; 87(1): 29 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Duarte, U. Schulte, A. V. Ushakova, and A. Videira Neurospora Strains Harboring Mitochondrial Disease-Associated Mutations in Iron-Sulfur Subunits of Complex I Genetics, September 1, 2005; 171(1): 91 - 99. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Eschemann, A. Galkin, W. Oettmeier, U. Brandt, and S. Kerscher HDQ (1-Hydroxy-2-dodecyl-4(1H)quinolone), a High Affinity Inhibitor for Mitochondrial Alternative NADH Dehydrogenase: EVIDENCE FOR A PING-PONG MECHANISM J. Biol. Chem., February 4, 2005; 280(5): 3138 - 3142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. P. Melo, T. M. Bandeiras, and M. Teixeira New Insights into Type II NAD(P)H:Quinone Oxidoreductases Microbiol. Mol. Biol. Rev., December 1, 2004; 68(4): 603 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Moore, R. J. Cook-Johnson, C. Rudhe, J. Whelan, D. A. Day, J. T. Wiskich, and K. L. Soole Identification of AtNDI1, an Internal Non-Phosphorylating NAD(P)H Dehydrogenase in Arabidopsis Mitochondria Plant Physiology, December 1, 2003; 133(4): 1968 - 1978. [Abstract] [Full Text]
|
|
|
|
|
|
A. M. Michalecka, A. S. Svensson, F. I. Johansson, S. C. Agius, U. Johanson, A. Brennicke, S. Binder, and A. G. Rasmusson Arabidopsis Genes Encoding Mitochondrial Type II NAD(P)H Dehydrogenases Have Different Evolutionary Origin and Show Distinct Responses to Light Plant Physiology, October 1, 2003; 133(2): 642 - 652. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |