|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 6, 4055-4062, February 9, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Nutrition, Case Western Reserve University
School of Medicine, Cleveland, Ohio 44106
To understand how glucose regulates the
expression of the glucose-6-phosphatase gene, the effect of glucose was
studied in primary cultures of rat hepatocytes. Glucose-6-phosphatase
mRNA levels increased about 10-fold when hepatocytes were incubated with 20 mM glucose. The rate of transcription of the
glucose-6-phosphatase gene increased about 3-fold in hepatocytes
incubated with glucose. The half-life of glucose-6-phosphatase mRNA
was estimated to be 90 min in the absence of glucose and 3 h in
its presence. Inhibition of the oxidative and the nonoxidative branches
of the pentose phosphate pathway blocked the stimulation of
glucose-6-phosphatase expression by glucose but not by xylitol or
carbohydrates that enter the glycolytic/gluconeogenic pathways at the
level of the triose phosphates. These results indicate that (i) the
glucose induction of the mRNA for the catalytic unit of
glucose-6-phosphatase occurs by transcriptional and
post-transcriptional mechanisms and that (ii) xylitol and glucose
increase the expression of this gene through different signaling pathways.
Regulation of the Glucose-6-phosphatase Gene by Glucose Occurs by
Transcriptional and Post-transcriptional Mechanisms
DIFFERENTIAL EFFECT OF GLUCOSE AND XYLITOL*
*
This work was supported by a Case Western Reserve
University Faculty Fellowship from the Mount Sinai Health Care
Foundation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Recipient of a Case Western Reserve University Faculty Fellowship
from the Mount Sinai Health Care Foundation. To whom correspondence and
reprint requests should be addressed: Dept. of Nutrition, Case Western
Reserve University School of Medicine, 10900 Euclid Ave., Cleveland OH
44106. Tel.: 216-368-2135; Fax: 216-368-6644; E-mail:
dxm71@po.cwru.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Azzout-Marniche, C. Gaudichon, C. Blouet, C. Bos, V. Mathe, J.-F. Huneau, and D. Tome Liver glyconeogenesis: a pathway to cope with postprandial amino acid excess in high-protein fed rats? Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2007; 292(4): R1400 - R1407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. B. Pedersen, P. Zhang, C. Doumen, M. Charbonnet, D. Lu, C. B. Newgard, J. W. Haycock, A. J. Lange, and D. K. Scott The promoter for the gene encoding the catalytic subunit of rat glucose-6-phosphatase contains two distinct glucose-responsive regions Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E788 - E801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gautier-Stein, C. Zitoun, E. Lalli, G. Mithieux, and F. Rajas Transcriptional Regulation of the Glucose-6-phosphatase Gene by cAMP/Vasoactive Intestinal Peptide in the Intestine: ROLE OF HNF4{alpha}, CREM, HNF1{alpha}, and C/EBP{alpha} J. Biol. Chem., October 20, 2006; 281(42): 31268 - 31278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Fujimoto, T. P. Torres, E. P. Donahue, and M. Shiota Glucose Toxicity Is Responsible for the Development of Impaired Regulation of Endogenous Glucose Production and Hepatic Glucokinase in Zucker Diabetic Fatty Rats Diabetes, September 1, 2006; 55(9): 2479 - 2490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Wang, Y. Wei, D. Schmoll, K. N. Maclean, and M. J. Pagliassotti Endoplasmic Reticulum Stress Increases Glucose-6-Phosphatase and Glucose Cycling in Liver Cells Endocrinology, January 1, 2006; 147(1): 350 - 358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Carriere, M. Le Gall, F. Gouyon-Saumande, D. Schmoll, E. Brot-Laroche, V. Chauffeton, J. Chambaz, and M. Rousset Intestinal Glucose-dependent Expression of Glucose-6-phosphatase: INVOLVEMENT OF THE ARYL RECEPTOR NUCLEAR TRANSLOCATOR TRANSCRIPTION FACTOR J. Biol. Chem., May 20, 2005; 280(20): 20094 - 20101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M C Salgado, I Meton, M Egea, and I V Baanante Transcriptional regulation of glucose-6-phosphatase catalytic subunit promoter by insulin and glucose in the carnivorous fish, Sparus aurata J. Mol. Endocrinol., December 1, 2004; 33(3): 783 - 795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-J. Shieh, C.-J. Pan, B. C. Mansfield, and J. Y. Chou A Potential New Role for Muscle in Blood Glucose Homeostasis J. Biol. Chem., June 18, 2004; 279(25): 26215 - 26219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Hornbuckle, C. A. Everett, C. C. Martin, S. S. Gustavson, C. A. Svitek, J. K. Oeser, D. W. Neal, A. D. Cherrington, and R. M. O'Brien Selective stimulation of G-6-Pase catalytic subunit but not G-6-P transporter gene expression by glucagon in vivo and cAMP in situ Am J Physiol Endocrinol Metab, May 1, 2004; 286(5): E795 - E808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wei, M. E. Bizeau, and M. J. Pagliassotti An Acute Increase in Fructose Concentration Increases Hepatic Glucose-6-Phosphatase mRNA via Mechanisms That Are Independent of Glycogen Synthase Kinase-3 in Rats J. Nutr., March 1, 2004; 134(3): 545 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Massillon, I. J. Arinze, C. Xu, and F. Bone Regulation of Glucose-6-phosphatase Gene Expression in Cultured Hepatocytes and H4IIE Cells by Short-chain Fatty Acids: ROLE OF HEPATIC NUCLEAR FACTOR-4{alpha} J. Biol. Chem., October 17, 2003; 278(42): 40694 - 40701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Collier, T.-T. T. Doan, M. C. Daniels, J. R. Schurr, J. K. Kolls, and D. K. Scott c-Myc Is Required for the Glucose-mediated Induction of Metabolic Enzyme Genes J. Biol. Chem., February 14, 2003; 278(8): 6588 - 6595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Pagliassotti, Y. Wei, and M. E. Bizeau Glucose-6-Phosphatase Activity Is Not Suppressed but the mRNA Level Is Increased by a Sucrose-Enriched Meal in Rats J. Nutr., January 1, 2003; 133(1): 32 - 37. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Marzban, R. Rahimian, R. W. Brownsey, and J. H. McNeill Mechanisms by which Bis(Maltolato)Oxovanadium(IV) Normalizes Phosphoenolpyruvate Carboxykinase and Glucose-6-Phosphatase Expression in Streptozotocin-Diabetic Rats in Vivo Endocrinology, December 1, 2002; 143(12): 4636 - 4645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Commerford, J. B. Ferniza, M. E. Bizeau, J. S. Thresher, W. T. Willis, and M. J. Pagliassotti Diets enriched in sucrose or fat increase gluconeogenesis and G-6-Pase but not basal glucose production in rats Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E545 - E555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Hornbuckle, D. S. Edgerton, J. E. Ayala, C. A. Svitek, J. K. Oeser, D. W. Neal, S. Cardin, A. D. Cherrington, and R. M. O'Brien Selective tonic inhibition of G-6-Pase catalytic subunit, but not G-6-P transporter, gene expression by insulin in vivo Am J Physiol Endocrinol Metab, October 1, 2001; 281(4): E713 - E725. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |