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J. Biol. Chem., Vol. 276, Issue 6, 4350-4356, February 9, 2001
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From the § Department of Biology, University of York,
P.O. Box 373, York YO10 5DD, United Kingdom and the ¶ Department
of Forest Genetics and Plant Physiology, the Swedish University of
Agricultural Sciences, S-901 83 Umeå, Sweden
Biochemical characterization of recombinant gene
products following a phylogenetic analysis of the
UDP-glucosyltransferase (UGT) multigene family of
Arabidopsis has identified one enzyme (UGT84B1) with high
activity toward the plant hormone indole-3-acetic acid (IAA) and three
related enzymes (UGT84B2, UGT75B1, and UGT75B2) with trace activities.
The identity of the IAA conjugate has been confirmed to be
1-O-indole acetyl glucose ester. A sequence annotated as a
UDP-glucose:IAA glucosyltransferase (IAA-UGT) in the
Arabidopsis genome and expressed sequence tag data
bases given its similarity to the maize iaglu gene sequence
showed no activity toward IAA. This study describes the first
biochemical analysis of a recombinant IAA-UGT and provides the
foundation for future genetic approaches to understand the role of
1-O-indole acetyl glucose ester in
Arabidopsis.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AC005106 (UGT75B1), AC005106 (UGT75B2), Z97335 (UGT75C1), AL161541 (UGT75D1), Z97339 (UGT84A1), AB019232 (UGT84A2), Z97339
(UGT84A3), Z97339 (UGT84A4), AC002391 (UGT84B1), and AC002391 (UGT84B2).
Identification and Biochemical Characterization of an
Arabidopsis Indole-3-acetic Acid Glucosyltransferase*
§,
*
This work was supported by Biotechnology and Biological
Sciences Research Council (BBSRC) grants (to D. J. B.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Recipient of a BBSRC-quota studentship.
To whom correspondence should be addressed. Tel.:
44-1904-434334; Fax: 44-1904-434336; E-mail: djb32@york.ac.uk.
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