Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M006917200 on November 21, 2000

J. Biol. Chem., Vol. 276, Issue 7, 5052-5058, February 16, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/7/5052    most recent
M006917200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saitoh, O.
Right arrow Articles by Kubo, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saitoh, O.
Right arrow Articles by Kubo, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Regulator of G Protein Signaling 8 (RGS8) Requires Its NH2 Terminus for Subcellular Localization and Acute Desensitization of G Protein-gated K+ Channels*

Osamu SaitohDagger §, Ikuo MasuhoDagger , Ion Terakawa, Satoshi Nomoto||, Tomiko Asano**, and Yoshihiro KuboDagger Dagger §§¶¶

From the Dagger  Department of Molecular and Cellular Neurobiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu-shi, Tokyo 183-8526, Japan, the  Hamamatsu University School of Medicine, Hamamatsu, Aichi 431-3192, Japan, the || Department of Health Science, Jichi Medical School and CREST, 3311-1 Yakushiji Minamikawachi, Tochigi 329-0498, Japan, the ** Department of Biochemistry, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi 480-03, Japan, the Dagger Dagger  Department of Neurophysiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu-shi, Tokyo 183-8526, Japan, and the §§ Department of Physiology, Tokyo Medical and Dental University, Graduate School and Faculty of Medicine, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan

Functional roles of the NH2-terminal region of RGS (regulators of G protein signaling) 8 in G protein signaling were studied. The deletion of the NH2-terminal region of RGS8 (Delta NRGS8) resulted in a partial loss of the inhibitory function in pheromone response of yeasts, although Galpha binding was not affected. To examine roles in subcellular distribution, we coexpressed two fusion proteins of RGS8-RFP and Delta NRGS8-GFP in DDT1MF2 cells. RGS8-RFP was highly concentrated in nuclei of unstimulated cells. Coexpression of constitutively active Galpha o resulted in translocation of RGS8 protein to the plasma membrane. In contrast, Delta NRGS8-GFP was distributed diffusely through the cytoplasm in the presence or absence of active Galpha o. When coexpressed with G protein-gated inwardly rectifying K+ channels, Delta NRGS8 accelerated both turning on and off similar to RGS8. Acute desensitization of G protein-gated inwardly rectifying K+ current observed in the presence of RGS8, however, was not induced by Delta NRGS8. Thus, we, for the first time, showed that the NH2 terminus of RGS8 contributes to the subcellular localization and to the desensitization of the G protein-coupled response.

* This work is supported in part by research grants from the Ministry of Education, Science, Sports and Culture of Japan (to O. S. and Y. K.) and from the Kato Memorial Bioscience Foundation (to O. S.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed: Dept. of Molecular and Cellular Neurobiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu-shi, Tokyo 183-8526, Japan. Tel.: 81-423-25-3881 (ext. 4058); Fax: 81-423-21-8678; E-mail: osaito@tmin.ac.jp.

¶¶ Supported by the Mitsubishi Foundation and Core Research for Evolutional Science and Technology of the Japan Science and Technology Corp.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Toro-Castillo, A. Thapliyal, H. Gonzalez-Ochoa, B. A. Adams, and U. Meza
Muscarinic modulation of Cav2.3 (R-type) calcium channels is antagonized by RGS3 and RGS3T
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C573 - C580.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
K. Bender, M.-C. Wellner-Kienitz, L. I Bosche, A. Rinne, C. Beckmann, and L. Pott
Acute desensitization of GIRK current in rat atrial myocytes is related to K+ current flow
J. Physiol., December 1, 2004; 561(2): 471 - 483.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
F. Jeanneteau, O. Guillin, J. Diaz, N. Griffon, and P. Sokoloff
GIPC Recruits GAIP (RGS19) To Attenuate Dopamine D2 Receptor Signaling
Mol. Biol. Cell, November 1, 2004; 15(11): 4926 - 4937.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Zhang, A. Dickson, and C. A. Doupnik
G{beta}{gamma}-activated Inwardly Rectifying K+ (GIRK) Channel Activation Kinetics via G{alpha}i and G{alpha}o-coupled Receptors Are Determined by G{alpha}-specific Interdomain Interactions That Affect GDP Release Rates
J. Biol. Chem., July 9, 2004; 279(28): 29787 - 29796.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Liu and R. A. Fisher
RGS6 Interacts with DMAP1 and DNMT1 and Inhibits DMAP1 Transcriptional Repressor Activity
J. Biol. Chem., April 2, 2004; 279(14): 14120 - 14128.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. K. Chatterjee, Z. Liu, and R. A. Fisher
Human RGS6 Gene Structure, Complex Alternative Splicing, and Role of N Terminus and G Protein {gamma}-Subunit-like (GGL) Domain in Subcellular Localization of RGS6 Splice Variants
J. Biol. Chem., August 8, 2003; 278(32): 30261 - 30271.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. K. Chatterjee and R. A. Fisher
Mild Heat and Proteotoxic Stress Promote Unique Subcellular Trafficking and Nucleolar Accumulation of RGS6 and Other RGS Proteins: ROLE OF THE RGS DOMAIN IN STRESS-INDUCED TRAFFICKING OF RGS PROTEINS
J. Biol. Chem., August 8, 2003; 278(32): 30272 - 30282.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Tosetti, N. Pathak, M. H. Jacob, and K. Dunlap
RGS3 mediates a calcium-dependent termination of G protein signaling in sensory neurons
PNAS, June 10, 2003; 100(12): 7337 - 7342.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Tosetti, T. Turner, Q. Lu, and K. Dunlap
Unique Isoform of Galpha -interacting Protein (RGS-GAIP) Selectively Discriminates between Two Go-mediated Pathways That Inhibit Ca2+ Channels
J. Biol. Chem., November 22, 2002; 277(48): 46001 - 46009.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
S. Hollinger and J. R. Hepler
Cellular Regulation of RGS Proteins: Modulators and Integrators of G Protein Signaling
Pharmacol. Rev., September 1, 2002; 54(3): 527 - 559.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Benzing, M. Kottgen, M. Johnson, B. Schermer, H. Zentgraf, G. Walz, and E. Kim
Interaction of 14-3-3 Protein with Regulator of G Protein Signaling 7 Is Dynamically Regulated by Tumor Necrosis Factor-alpha
J. Biol. Chem., August 30, 2002; 277(36): 32954 - 32962.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Hu and T. G. Wensel
R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein, RGS9-1
PNAS, July 23, 2002; 99(15): 9755 - 9760.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
O. Saitoh, Y. Murata, M. Odagiri, M. Itoh, H. Itoh, T. Misaka, and Y. Kubo
Alternative splicing of RGS8 gene determines inhibitory function of receptor type-specific Gq signaling
PNAS, July 23, 2002; 99(15): 10138 - 10143.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. K. Chatterjee and R. A. Fisher
RGS12TS-S Localizes at Nuclear Matrix-Associated Subnuclear Structures and Represses Transcription: Structural Requirements for Subnuclear Targeting and Transcriptional Repression
Mol. Cell. Biol., June 15, 2002; 22(12): 4334 - 4345.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. B. Blumer, L. J. Chandler, and S. M. Lanier
Expression Analysis and Subcellular Distribution of the Two G-protein Regulators AGS3 and LGN Indicate Distinct Functionality. LOCALIZATION OF LGN TO THE MIDBODY DURING CYTOKINESIS
J. Biol. Chem., May 3, 2002; 277(18): 15897 - 15903.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S.-W. Jeong and S. R Ikeda
Differential regulation of G protein-gated inwardly rectifying K+ channel kinetics by distinct domains of RGS8
J. Physiol., September 1, 2001; 535(2): 335 - 347.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement