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J. Biol. Chem., Vol. 276, Issue 7, 5235-5239, February 16, 2001
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From the Directed cell migration occurs in response to
extracellular cues. Following stimulation of a cell with
chemoattractant, a significant rearrangement of the actin cytoskeleton
is mediated by intracellular signaling pathways and results in
polarization of the cell and movement via pseudopod extension. Amoeboid
myosin Is play a critical role in regulating pseudopod formation in
Dictyostelium, and their activity is activated by heavy
chain phosphorylation. The effect of chemotactic stimulation on the
in vivo phosphorylation level of a
Dictyostelium myosin I, myoB, was tested. The myoB heavy
chain is phosphorylated in vivo on serine 322 (the myosin TEDS rule phosphorylation site) in chemotactically competent cells. The
level of myoB phosphorylation increases following stimulation of
starving cells with the chemoattractant cAMP. A 3-fold peak increase in
the level of phosphorylation is observed at 60 s following stimulation, a time at which the Dictyostelium cell
actively extends pseudopodia. These findings suggest that chemotactic
stimulation results in increased myoB activity via heavy chain
phosphorylation and contributes to the global extension of pseudopodia
that occurs prior to polarization and directed motility.
Myosin I Phosphorylation Is Increased by Chemotactic
Stimulation*
§,,, and¶
Department of Cell Biology, Duke University
Medical Center, Durham, North Carolina 27710 and the ¶ Department
of Genetics, Cell Biology, and Development, University of Minnesota,
Minneapolis, Minnesota 55455
*
The work was supported by National Institutes of Health
Grants F32-GM16090 (to N. R. G.) and GM46486 (to M. A. T.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom all correspondence should be addressed: Dept. of
Genetics, Cell Biology and Development, 6-160 Jackson Hall, 321 Church St. S.E., University of Minnesota, Minneapolis, MN 55455. Tel.: 612-625-8498; Fax: 612-624-8118; E-mail:
titus@lenti.med.umn.edu.
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